Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus

Abstract To establish an efficient plant regeneration system from cell suspension cultures of Euonymus alatus, embryogenic callus formation from immature embryos was investigated. The highest frequency of embryogenic callus formation reached 50% when the immature zygotic embryos were incubated on Mu...

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Autores principales:	Hyun-A Woo, Seong Sub Ku, Eun Yee Jie, HyeRan Kim, Hyun-Soon Kim, Hye Sun Cho, Won-Joong Jeong, Sang Un Park, Sung Ran Min, Suk Weon Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0431f00fb2e44e1196202f15786274b2
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spelling	oai:doaj.org-article:0431f00fb2e44e1196202f15786274b22021-12-02T16:17:21ZEfficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus10.1038/s41598-021-94597-42045-2322https://doaj.org/article/0431f00fb2e44e1196202f15786274b22021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94597-4https://doaj.org/toc/2045-2322Abstract To establish an efficient plant regeneration system from cell suspension cultures of Euonymus alatus, embryogenic callus formation from immature embryos was investigated. The highest frequency of embryogenic callus formation reached 50% when the immature zygotic embryos were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). At higher concentrations of 2,4-D (over 2 mg/L), the frequency of embryogenic callus formation declined significantly. The total number of somatic embryos development was highest with the 3% (w/v) sucrose treatment, which was found to be the optimal concentration for somatic embryo formation. Activated charcoal (AC) and 6-benzyladenine (BA) significantly increased the frequency of plantlet conversion from somatic embryos, but gibberellic acid (GA3) had a negative effect on plantlet conversion and subsequent development from somatic embryos. Even though the cell suspension cultures were maintained for more than 1 year, cell aggregates from embryogenic cell suspension cultures were successfully converted into normal somatic embryos with two cotyledons. To our knowledge, this is the first successful report of a plant regeneration system of E. alatus via somatic embryogenesis. Thus, the embryogenic cell line and plant regeneration system established in this study can be applied to mass proliferation and production of pharmaceutical metabolite in E. alatus.Hyun-A WooSeong Sub KuEun Yee JieHyeRan KimHyun-Soon KimHye Sun ChoWon-Joong JeongSang Un ParkSung Ran MinSuk Weon KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyun-A Woo Seong Sub Ku Eun Yee Jie HyeRan Kim Hyun-Soon Kim Hye Sun Cho Won-Joong Jeong Sang Un Park Sung Ran Min Suk Weon Kim Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus
description	Abstract To establish an efficient plant regeneration system from cell suspension cultures of Euonymus alatus, embryogenic callus formation from immature embryos was investigated. The highest frequency of embryogenic callus formation reached 50% when the immature zygotic embryos were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). At higher concentrations of 2,4-D (over 2 mg/L), the frequency of embryogenic callus formation declined significantly. The total number of somatic embryos development was highest with the 3% (w/v) sucrose treatment, which was found to be the optimal concentration for somatic embryo formation. Activated charcoal (AC) and 6-benzyladenine (BA) significantly increased the frequency of plantlet conversion from somatic embryos, but gibberellic acid (GA3) had a negative effect on plantlet conversion and subsequent development from somatic embryos. Even though the cell suspension cultures were maintained for more than 1 year, cell aggregates from embryogenic cell suspension cultures were successfully converted into normal somatic embryos with two cotyledons. To our knowledge, this is the first successful report of a plant regeneration system of E. alatus via somatic embryogenesis. Thus, the embryogenic cell line and plant regeneration system established in this study can be applied to mass proliferation and production of pharmaceutical metabolite in E. alatus.
format	article
author	Hyun-A Woo Seong Sub Ku Eun Yee Jie HyeRan Kim Hyun-Soon Kim Hye Sun Cho Won-Joong Jeong Sang Un Park Sung Ran Min Suk Weon Kim
author_facet	Hyun-A Woo Seong Sub Ku Eun Yee Jie HyeRan Kim Hyun-Soon Kim Hye Sun Cho Won-Joong Jeong Sang Un Park Sung Ran Min Suk Weon Kim
author_sort	Hyun-A Woo
title	Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus
title_short	Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus
title_full	Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus
title_fullStr	Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus
title_full_unstemmed	Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus
title_sort	efficient plant regeneration from embryogenic cell suspension cultures of euonymus alatus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0431f00fb2e44e1196202f15786274b2
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Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus

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