MiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression

Abstract Distraction osteogenesis (DO) is a unique technique for promoting bone formation in clinical practice. However the underlying mechanism remains elusive. As epigenetic mediators, microRNAs have been reported to play important roles in regulating osteogenesis. In this study, after successfull...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuxin Sun, Jia Xu, Liangliang Xu, Jinfang Zhang, Kaiming Chan, Xiaohua Pan, Gang Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/043522b641d740558735693d5fad70a1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:043522b641d740558735693d5fad70a1
record_format	dspace
spelling	oai:doaj.org-article:043522b641d740558735693d5fad70a12021-12-02T16:07:59ZMiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression10.1038/s41598-017-00466-42045-2322https://doaj.org/article/043522b641d740558735693d5fad70a12017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00466-4https://doaj.org/toc/2045-2322Abstract Distraction osteogenesis (DO) is a unique technique for promoting bone formation in clinical practice. However the underlying mechanism remains elusive. As epigenetic mediators, microRNAs have been reported to play important roles in regulating osteogenesis. In this study, after successfully established the DO model of rats, a microRNA microarray was performed to find molecular targets for DO. Total 100 microRNAs were identified as differently expressed, with miR-503 being one of the most significantly up-regulated miRNAs in DO. The further investigation also showed that miR-503 was upregulated during osteogenesis in mesenchymal stem cells of rats, and overexpression of miR-503 significantly promoted osteogenesis in vitro and accelerated mineralization in DO process in vivo. By using bioinformatic investigations and luciferase activities, we successfully demonstrated that Smurf1, a negative regulator of osteogenesis, was a real target of miR-503. Furthermore, Smurf1 knockdown promoted osteogenesis and antagomir-503 abolished the promotive effect, suggesting that miR-503 mediated osteogenic differentiation via suppressing Smurf1 expression. To sum up, these findings indicated that miR-503 promoted osteogenesis and accelerated bone formation, which may shed light on the development for a potential therapeutic target for bone repair.Yuxin SunJia XuLiangliang XuJinfang ZhangKaiming ChanXiaohua PanGang LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuxin Sun Jia Xu Liangliang Xu Jinfang Zhang Kaiming Chan Xiaohua Pan Gang Li MiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression
description	Abstract Distraction osteogenesis (DO) is a unique technique for promoting bone formation in clinical practice. However the underlying mechanism remains elusive. As epigenetic mediators, microRNAs have been reported to play important roles in regulating osteogenesis. In this study, after successfully established the DO model of rats, a microRNA microarray was performed to find molecular targets for DO. Total 100 microRNAs were identified as differently expressed, with miR-503 being one of the most significantly up-regulated miRNAs in DO. The further investigation also showed that miR-503 was upregulated during osteogenesis in mesenchymal stem cells of rats, and overexpression of miR-503 significantly promoted osteogenesis in vitro and accelerated mineralization in DO process in vivo. By using bioinformatic investigations and luciferase activities, we successfully demonstrated that Smurf1, a negative regulator of osteogenesis, was a real target of miR-503. Furthermore, Smurf1 knockdown promoted osteogenesis and antagomir-503 abolished the promotive effect, suggesting that miR-503 mediated osteogenic differentiation via suppressing Smurf1 expression. To sum up, these findings indicated that miR-503 promoted osteogenesis and accelerated bone formation, which may shed light on the development for a potential therapeutic target for bone repair.
format	article
author	Yuxin Sun Jia Xu Liangliang Xu Jinfang Zhang Kaiming Chan Xiaohua Pan Gang Li
author_facet	Yuxin Sun Jia Xu Liangliang Xu Jinfang Zhang Kaiming Chan Xiaohua Pan Gang Li
author_sort	Yuxin Sun
title	MiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression
title_short	MiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression
title_full	MiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression
title_fullStr	MiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression
title_full_unstemmed	MiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression
title_sort	mir-503 promotes bone formation in distraction osteogenesis through suppressing smurf1 expression
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/043522b641d740558735693d5fad70a1
work_keys_str_mv	AT yuxinsun mir503promotesboneformationindistractionosteogenesisthroughsuppressingsmurf1expression AT jiaxu mir503promotesboneformationindistractionosteogenesisthroughsuppressingsmurf1expression AT liangliangxu mir503promotesboneformationindistractionosteogenesisthroughsuppressingsmurf1expression AT jinfangzhang mir503promotesboneformationindistractionosteogenesisthroughsuppressingsmurf1expression AT kaimingchan mir503promotesboneformationindistractionosteogenesisthroughsuppressingsmurf1expression AT xiaohuapan mir503promotesboneformationindistractionosteogenesisthroughsuppressingsmurf1expression AT gangli mir503promotesboneformationindistractionosteogenesisthroughsuppressingsmurf1expression
_version_	1718384636207300608

MiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression

Ejemplares similares