Musarin, a novel protein with tyrosine kinase inhibitory activity from Trametes versicolor, inhibits colorectal cancer stem cell growth

Colorectal cancer is the second deadly cancer in the world. Trametes versicolor is a traditional Chinese medicinal mushroom with a long history of being used to regulate immunity and prevent cancer. Trametes versicolor mushroom extract demonstrates strongly cell growth inhibitory activity on human c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	YingYing He, Shubai Liu, David S. Newburg
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Trametes versicolor Cancer stem cell Tyrosine kinase inhibitor EGFR Colorectal cancer Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/043709064d944a509d20c13ae83b14aa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:043709064d944a509d20c13ae83b14aa
record_format	dspace
spelling	oai:doaj.org-article:043709064d944a509d20c13ae83b14aa2021-11-14T04:30:00ZMusarin, a novel protein with tyrosine kinase inhibitory activity from Trametes versicolor, inhibits colorectal cancer stem cell growth0753-332210.1016/j.biopha.2021.112339https://doaj.org/article/043709064d944a509d20c13ae83b14aa2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221011239https://doaj.org/toc/0753-3322Colorectal cancer is the second deadly cancer in the world. Trametes versicolor is a traditional Chinese medicinal mushroom with a long history of being used to regulate immunity and prevent cancer. Trametes versicolor mushroom extract demonstrates strongly cell growth inhibitory activity on human colorectal tumor cells. In this study, we characterized a novel 12-kDa protein that named musarin, which was purified from Trametes versicolor mushroom extract and showed significant growth inhibition on multiple human colorectal cancer cell lines in vitro. The protein sequence of musarin was determined through enzyme digestion and MS/MS analysis. Furthermore, Musarin, in particular, strongly inhibits aggressive human colorectal cancer stem cell-like CD24+CD44+ HT29 proliferation in vitro and in a NOD/SCID murine xenograft model. Through whole transcription profile and gene enrichment analysis of musarin-treated CSCs-like cells, major signaling pathways and network modulated by musarin have been enriched, including the bioprocess of the Epithelial-Mesenchymal Transition, the EGFR-Ras signaling pathway and enzyme inhibitor activity. Musarin demonstrated tyrosine kinase inhibitory activity in vitro. Musarin strongly attenuated EGFR expression and down-regulated phosphorylation level, thereby slowing cancer cells proliferation. In addition, oral ingestion of musarin significantly inhibited CD24+CD44+ HT29 generated tumor development in SCID/NOD mice with less side effects in microgram doses. Targeting self-renewal aggressive stem-cell like cancer cell proliferation, with higher water solubility and lower cytotoxicity, musarin has shown strong potence to be developed as a promising novel therapeutic drug candidate against colorectal cancers, especially those that acquire chemo-resistance.YingYing HeShubai LiuDavid S. NewburgElsevierarticleTrametes versicolorCancer stem cellTyrosine kinase inhibitorEGFRColorectal cancerTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112339- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Trametes versicolor Cancer stem cell Tyrosine kinase inhibitor EGFR Colorectal cancer Therapeutics. Pharmacology RM1-950
spellingShingle	Trametes versicolor Cancer stem cell Tyrosine kinase inhibitor EGFR Colorectal cancer Therapeutics. Pharmacology RM1-950 YingYing He Shubai Liu David S. Newburg Musarin, a novel protein with tyrosine kinase inhibitory activity from Trametes versicolor, inhibits colorectal cancer stem cell growth
description	Colorectal cancer is the second deadly cancer in the world. Trametes versicolor is a traditional Chinese medicinal mushroom with a long history of being used to regulate immunity and prevent cancer. Trametes versicolor mushroom extract demonstrates strongly cell growth inhibitory activity on human colorectal tumor cells. In this study, we characterized a novel 12-kDa protein that named musarin, which was purified from Trametes versicolor mushroom extract and showed significant growth inhibition on multiple human colorectal cancer cell lines in vitro. The protein sequence of musarin was determined through enzyme digestion and MS/MS analysis. Furthermore, Musarin, in particular, strongly inhibits aggressive human colorectal cancer stem cell-like CD24+CD44+ HT29 proliferation in vitro and in a NOD/SCID murine xenograft model. Through whole transcription profile and gene enrichment analysis of musarin-treated CSCs-like cells, major signaling pathways and network modulated by musarin have been enriched, including the bioprocess of the Epithelial-Mesenchymal Transition, the EGFR-Ras signaling pathway and enzyme inhibitor activity. Musarin demonstrated tyrosine kinase inhibitory activity in vitro. Musarin strongly attenuated EGFR expression and down-regulated phosphorylation level, thereby slowing cancer cells proliferation. In addition, oral ingestion of musarin significantly inhibited CD24+CD44+ HT29 generated tumor development in SCID/NOD mice with less side effects in microgram doses. Targeting self-renewal aggressive stem-cell like cancer cell proliferation, with higher water solubility and lower cytotoxicity, musarin has shown strong potence to be developed as a promising novel therapeutic drug candidate against colorectal cancers, especially those that acquire chemo-resistance.
format	article
author	YingYing He Shubai Liu David S. Newburg
author_facet	YingYing He Shubai Liu David S. Newburg
author_sort	YingYing He
title	Musarin, a novel protein with tyrosine kinase inhibitory activity from Trametes versicolor, inhibits colorectal cancer stem cell growth
title_short	Musarin, a novel protein with tyrosine kinase inhibitory activity from Trametes versicolor, inhibits colorectal cancer stem cell growth
title_full	Musarin, a novel protein with tyrosine kinase inhibitory activity from Trametes versicolor, inhibits colorectal cancer stem cell growth
title_fullStr	Musarin, a novel protein with tyrosine kinase inhibitory activity from Trametes versicolor, inhibits colorectal cancer stem cell growth
title_full_unstemmed	Musarin, a novel protein with tyrosine kinase inhibitory activity from Trametes versicolor, inhibits colorectal cancer stem cell growth
title_sort	musarin, a novel protein with tyrosine kinase inhibitory activity from trametes versicolor, inhibits colorectal cancer stem cell growth
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/043709064d944a509d20c13ae83b14aa
work_keys_str_mv	AT yingyinghe musarinanovelproteinwithtyrosinekinaseinhibitoryactivityfromtrametesversicolorinhibitscolorectalcancerstemcellgrowth AT shubailiu musarinanovelproteinwithtyrosinekinaseinhibitoryactivityfromtrametesversicolorinhibitscolorectalcancerstemcellgrowth AT davidsnewburg musarinanovelproteinwithtyrosinekinaseinhibitoryactivityfromtrametesversicolorinhibitscolorectalcancerstemcellgrowth
_version_	1718429997360742400

Musarin, a novel protein with tyrosine kinase inhibitory activity from Trametes versicolor, inhibits colorectal cancer stem cell growth

Ejemplares similares