Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals

Abstract To identify circulating proteins predictive of acute cardiovascular disease events in the general population, we performed a proteomic screen in plasma from asymptomatic individuals. A “Discovery cohort” of 25 individuals who subsequently incurred a cardiovascular event within 3 years (medi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Moritz Lassé, Anna P. Pilbrow, Torsten Kleffmann, Elin Andersson Överström, Anne von Zychlinski, Christopher M. A. Frampton, Katrina K. Poppe, Richard W. Troughton, Lynley K. Lewis, Timothy C. R. Prickett, Christopher J. Pemberton, Arthur M. Richards, Vicky A. Cameron
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/043710be91bb407197c17d86d6c4b4e8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:043710be91bb407197c17d86d6c4b4e8
record_format	dspace
spelling	oai:doaj.org-article:043710be91bb407197c17d86d6c4b4e82021-12-02T13:20:22ZFibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals10.1038/s41598-021-84046-72045-2322https://doaj.org/article/043710be91bb407197c17d86d6c4b4e82021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84046-7https://doaj.org/toc/2045-2322Abstract To identify circulating proteins predictive of acute cardiovascular disease events in the general population, we performed a proteomic screen in plasma from asymptomatic individuals. A “Discovery cohort” of 25 individuals who subsequently incurred a cardiovascular event within 3 years (median age = 70 years, 80% male) was matched to 25 controls remaining event-free for > 5 years (median age = 72 years, 80% male). Plasma proteins were assessed by data independent acquisition mass spectrometry (DIA-MS). Associations with cardiovascular events were tested using Cox regression, adjusted for the New Zealand Cardiovascular Risk Score. Concentrations of leading protein candidates were subsequently measured with ELISAs in a larger (n = 151) independent subset. In the Discovery cohort, 76 plasma proteins were robustly quantified by DIA-MS, with 8 independently associated with cardiovascular events. These included (HR = hazard ratio [95% confidence interval] above vs below median): fibrinogen alpha chain (HR = 1.84 [1.19–2.84]); alpha-2-HS-glycoprotein (also called fetuin A) (HR = 1.86 [1.19–2.93]); clusterin isoform 2 (HR = 1.59 [1.06–2.38]); fibrinogen beta chain (HR = 1.55 [1.04–2.30]); hemoglobin subunit beta (HR = 1.49 [1.04–2.15]); complement component C9 (HR = 1.62 [1.01–2.59]), fibronectin isoform 3 (HR = 0.60 [0.37–0.99]); and lipopolysaccharide-binding protein (HR = 1.58 [1.00–2.49]). The proteins for which DIA-MS and ELISA data were correlated, fibrinogen and hemoglobin, were analyzed in an Extended cohort, with broader inclusion criteria and longer time to events, in which these two proteins were not associated with incident cardiovascular events. We have identified eight candidate proteins that may independently predict cardiovascular events occurring within three years in asymptomatic, low-to-moderate risk individuals, although these appear not to predict events beyond three years.Moritz LasséAnna P. PilbrowTorsten KleffmannElin Andersson ÖverströmAnne von ZychlinskiChristopher M. A. FramptonKatrina K. PoppeRichard W. TroughtonLynley K. LewisTimothy C. R. PrickettChristopher J. PembertonArthur M. RichardsVicky A. CameronNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Moritz Lassé Anna P. Pilbrow Torsten Kleffmann Elin Andersson Överström Anne von Zychlinski Christopher M. A. Frampton Katrina K. Poppe Richard W. Troughton Lynley K. Lewis Timothy C. R. Prickett Christopher J. Pemberton Arthur M. Richards Vicky A. Cameron Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals
description	Abstract To identify circulating proteins predictive of acute cardiovascular disease events in the general population, we performed a proteomic screen in plasma from asymptomatic individuals. A “Discovery cohort” of 25 individuals who subsequently incurred a cardiovascular event within 3 years (median age = 70 years, 80% male) was matched to 25 controls remaining event-free for > 5 years (median age = 72 years, 80% male). Plasma proteins were assessed by data independent acquisition mass spectrometry (DIA-MS). Associations with cardiovascular events were tested using Cox regression, adjusted for the New Zealand Cardiovascular Risk Score. Concentrations of leading protein candidates were subsequently measured with ELISAs in a larger (n = 151) independent subset. In the Discovery cohort, 76 plasma proteins were robustly quantified by DIA-MS, with 8 independently associated with cardiovascular events. These included (HR = hazard ratio [95% confidence interval] above vs below median): fibrinogen alpha chain (HR = 1.84 [1.19–2.84]); alpha-2-HS-glycoprotein (also called fetuin A) (HR = 1.86 [1.19–2.93]); clusterin isoform 2 (HR = 1.59 [1.06–2.38]); fibrinogen beta chain (HR = 1.55 [1.04–2.30]); hemoglobin subunit beta (HR = 1.49 [1.04–2.15]); complement component C9 (HR = 1.62 [1.01–2.59]), fibronectin isoform 3 (HR = 0.60 [0.37–0.99]); and lipopolysaccharide-binding protein (HR = 1.58 [1.00–2.49]). The proteins for which DIA-MS and ELISA data were correlated, fibrinogen and hemoglobin, were analyzed in an Extended cohort, with broader inclusion criteria and longer time to events, in which these two proteins were not associated with incident cardiovascular events. We have identified eight candidate proteins that may independently predict cardiovascular events occurring within three years in asymptomatic, low-to-moderate risk individuals, although these appear not to predict events beyond three years.
format	article
author	Moritz Lassé Anna P. Pilbrow Torsten Kleffmann Elin Andersson Överström Anne von Zychlinski Christopher M. A. Frampton Katrina K. Poppe Richard W. Troughton Lynley K. Lewis Timothy C. R. Prickett Christopher J. Pemberton Arthur M. Richards Vicky A. Cameron
author_facet	Moritz Lassé Anna P. Pilbrow Torsten Kleffmann Elin Andersson Överström Anne von Zychlinski Christopher M. A. Frampton Katrina K. Poppe Richard W. Troughton Lynley K. Lewis Timothy C. R. Prickett Christopher J. Pemberton Arthur M. Richards Vicky A. Cameron
author_sort	Moritz Lassé
title	Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals
title_short	Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals
title_full	Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals
title_fullStr	Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals
title_full_unstemmed	Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals
title_sort	fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/043710be91bb407197c17d86d6c4b4e8
work_keys_str_mv	AT moritzlasse fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT annappilbrow fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT torstenkleffmann fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT elinanderssonoverstrom fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT annevonzychlinski fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT christophermaframpton fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT katrinakpoppe fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT richardwtroughton fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT lynleyklewis fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT timothycrprickett fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT christopherjpemberton fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT arthurmrichards fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals AT vickyacameron fibrinogenandhemoglobinpredictnearfuturecardiovasculareventsinasymptomaticindividuals
_version_	1718393190990479360

Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals

Ejemplares similares