Nutrition and the Gut Microbiota in 10- to 18-Month-Old Children Living in Urban Slums of Mumbai, India
ABSTRACT In this cross-sectional study, we describe the composition and diversity of the gut microbiota among undernourished children living in urban slums of Mumbai, India, and determine how nutritional status, including anthropometric measurements, dietary intakes from complementary foods, feeding...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
American Society for Microbiology
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/043ca626a00c444fabd5dd50be30bac8 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:043ca626a00c444fabd5dd50be30bac8 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:043ca626a00c444fabd5dd50be30bac82021-11-15T15:30:58ZNutrition and the Gut Microbiota in 10- to 18-Month-Old Children Living in Urban Slums of Mumbai, India10.1128/mSphere.00731-202379-5042https://doaj.org/article/043ca626a00c444fabd5dd50be30bac82020-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00731-20https://doaj.org/toc/2379-5042ABSTRACT In this cross-sectional study, we describe the composition and diversity of the gut microbiota among undernourished children living in urban slums of Mumbai, India, and determine how nutritional status, including anthropometric measurements, dietary intakes from complementary foods, feeding practices, and micronutrient concentrations, is associated with their gut microbiota. We collected rectal swabs from children aged 10 to 18 months living in urban slums of Mumbai participating in a randomized controlled feeding trial and conducted 16S rRNA sequencing to determine the composition of the gut microbiota. Across the study cohort, Proteobacteria dominated the gut microbiota at over 80% relative abundance, with Actinobacteria representation at <4%, suggesting immaturity of the gut. Increased microbial α-diversity was associated with current breastfeeding, greater head circumference, higher fat intake, and lower hemoglobin concentration and weight-for-length Z-score. In redundancy analyses, 47% of the variation in Faith’s phylogenetic diversity (Faith’s PD) could be accounted for by age and by iron and polyunsaturated fatty acid intakes. Differences in community structure (β-diversity) of the microbiota were observed among those consuming fats and oils the previous day compared to those not consuming fats and oils the previous day. Our findings suggest that growth, diet, and feeding practices are associated with gut microbiota metrics in undernourished children, whose gut microbiota were comprised mainly of Proteobacteria, a phylum containing many potentially pathogenic taxa. IMPORTANCE The impact of comprehensive nutritional status, defined as growth, nutritional blood biomarkers, dietary intakes, and feeding practices, on the gut microbiome in children living in low-resource settings has remained underreported in microbiome research. Among undernourished children living in urban slums of Mumbai, India, we observed a high relative abundance of Proteobacteria, a phylum including many potentially pathogenic species similar to the composition in preterm infants, suggesting immaturity of the gut, or potentially a high inflammatory burden. We found head circumference, fat and iron intake, and current breastfeeding were positively associated with microbial diversity, while hemoglobin and weight for length were associated with lower diversity. Findings suggest that examining comprehensive nutrition is critical to gain more understanding of how nutrition and the gut microbiota are linked, particularly in vulnerable populations such as children in urban slum settings.Samantha L. HueyLingjing JiangMarcus W. FedarkoDaniel McDonaldCameron MartinoFarhana AliDavid G. RussellShobha A. UdipiAparna ThoratVarsha ThakkerPadmini GhugreR. D. PotdarHarsha ChopraKripa RajagopalanJere D. HaasJulia L. FinkelsteinRob KnightSaurabh MehtaAmerican Society for Microbiologyarticleinfantchildgrowthdietnutritionfeeding practicesMicrobiologyQR1-502ENmSphere, Vol 5, Iss 5 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
infant child growth diet nutrition feeding practices Microbiology QR1-502 |
| spellingShingle |
infant child growth diet nutrition feeding practices Microbiology QR1-502 Samantha L. Huey Lingjing Jiang Marcus W. Fedarko Daniel McDonald Cameron Martino Farhana Ali David G. Russell Shobha A. Udipi Aparna Thorat Varsha Thakker Padmini Ghugre R. D. Potdar Harsha Chopra Kripa Rajagopalan Jere D. Haas Julia L. Finkelstein Rob Knight Saurabh Mehta Nutrition and the Gut Microbiota in 10- to 18-Month-Old Children Living in Urban Slums of Mumbai, India |
| description |
ABSTRACT In this cross-sectional study, we describe the composition and diversity of the gut microbiota among undernourished children living in urban slums of Mumbai, India, and determine how nutritional status, including anthropometric measurements, dietary intakes from complementary foods, feeding practices, and micronutrient concentrations, is associated with their gut microbiota. We collected rectal swabs from children aged 10 to 18 months living in urban slums of Mumbai participating in a randomized controlled feeding trial and conducted 16S rRNA sequencing to determine the composition of the gut microbiota. Across the study cohort, Proteobacteria dominated the gut microbiota at over 80% relative abundance, with Actinobacteria representation at <4%, suggesting immaturity of the gut. Increased microbial α-diversity was associated with current breastfeeding, greater head circumference, higher fat intake, and lower hemoglobin concentration and weight-for-length Z-score. In redundancy analyses, 47% of the variation in Faith’s phylogenetic diversity (Faith’s PD) could be accounted for by age and by iron and polyunsaturated fatty acid intakes. Differences in community structure (β-diversity) of the microbiota were observed among those consuming fats and oils the previous day compared to those not consuming fats and oils the previous day. Our findings suggest that growth, diet, and feeding practices are associated with gut microbiota metrics in undernourished children, whose gut microbiota were comprised mainly of Proteobacteria, a phylum containing many potentially pathogenic taxa. IMPORTANCE The impact of comprehensive nutritional status, defined as growth, nutritional blood biomarkers, dietary intakes, and feeding practices, on the gut microbiome in children living in low-resource settings has remained underreported in microbiome research. Among undernourished children living in urban slums of Mumbai, India, we observed a high relative abundance of Proteobacteria, a phylum including many potentially pathogenic species similar to the composition in preterm infants, suggesting immaturity of the gut, or potentially a high inflammatory burden. We found head circumference, fat and iron intake, and current breastfeeding were positively associated with microbial diversity, while hemoglobin and weight for length were associated with lower diversity. Findings suggest that examining comprehensive nutrition is critical to gain more understanding of how nutrition and the gut microbiota are linked, particularly in vulnerable populations such as children in urban slum settings. |
| format |
article |
| author |
Samantha L. Huey Lingjing Jiang Marcus W. Fedarko Daniel McDonald Cameron Martino Farhana Ali David G. Russell Shobha A. Udipi Aparna Thorat Varsha Thakker Padmini Ghugre R. D. Potdar Harsha Chopra Kripa Rajagopalan Jere D. Haas Julia L. Finkelstein Rob Knight Saurabh Mehta |
| author_facet |
Samantha L. Huey Lingjing Jiang Marcus W. Fedarko Daniel McDonald Cameron Martino Farhana Ali David G. Russell Shobha A. Udipi Aparna Thorat Varsha Thakker Padmini Ghugre R. D. Potdar Harsha Chopra Kripa Rajagopalan Jere D. Haas Julia L. Finkelstein Rob Knight Saurabh Mehta |
| author_sort |
Samantha L. Huey |
| title |
Nutrition and the Gut Microbiota in 10- to 18-Month-Old Children Living in Urban Slums of Mumbai, India |
| title_short |
Nutrition and the Gut Microbiota in 10- to 18-Month-Old Children Living in Urban Slums of Mumbai, India |
| title_full |
Nutrition and the Gut Microbiota in 10- to 18-Month-Old Children Living in Urban Slums of Mumbai, India |
| title_fullStr |
Nutrition and the Gut Microbiota in 10- to 18-Month-Old Children Living in Urban Slums of Mumbai, India |
| title_full_unstemmed |
Nutrition and the Gut Microbiota in 10- to 18-Month-Old Children Living in Urban Slums of Mumbai, India |
| title_sort |
nutrition and the gut microbiota in 10- to 18-month-old children living in urban slums of mumbai, india |
| publisher |
American Society for Microbiology |
| publishDate |
2020 |
| url |
https://doaj.org/article/043ca626a00c444fabd5dd50be30bac8 |
| work_keys_str_mv |
AT samanthalhuey nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT lingjingjiang nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT marcuswfedarko nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT danielmcdonald nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT cameronmartino nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT farhanaali nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT davidgrussell nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT shobhaaudipi nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT aparnathorat nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT varshathakker nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT padminighugre nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT rdpotdar nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT harshachopra nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT kriparajagopalan nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT jeredhaas nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT julialfinkelstein nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT robknight nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia AT saurabhmehta nutritionandthegutmicrobiotain10to18montholdchildrenlivinginurbanslumsofmumbaiindia |
| _version_ |
1718427866877657088 |