A newly developed cloning technique in sturgeons; an important step towards recovering endangered species

Abstract Several steps of sturgeon somatic cell nuclear transfer (SCNT) have been recently established, but improvements are needed to make it a feasible tool to preserve the natural populations of this group of endangered species. The donor cell position inside the recipient egg seems to be crucial...

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Autores principales:	Effrosyni Fatira, Miloš Havelka, Catherine Labbé, Alexandra Depincé, Martin Pšenička, Taiju Saito
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/04402796dafb487eb4828353581b7bc3
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spelling	oai:doaj.org-article:04402796dafb487eb4828353581b7bc32021-12-02T15:09:16ZA newly developed cloning technique in sturgeons; an important step towards recovering endangered species10.1038/s41598-019-46892-42045-2322https://doaj.org/article/04402796dafb487eb4828353581b7bc32019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46892-4https://doaj.org/toc/2045-2322Abstract Several steps of sturgeon somatic cell nuclear transfer (SCNT) have been recently established, but improvements are needed to make it a feasible tool to preserve the natural populations of this group of endangered species. The donor cell position inside the recipient egg seems to be crucial for its reprogramming; therefore by injecting multiple donor somatic cells instead of a single cell with a single manipulation, we increased the potential for embryo development. Using the Russian sturgeon Acipenser gueldenstaedtii as a multiple cell donor and sterlet Acipenser ruthenus as the non-enucleated egg recipient, we obtained higher proportion of eggs developing into embryos than previously reported with single-SCNT. Molecular data showed the production of a specimen (0.8%) contained only the donor genome with no contribution from the recipient, while two specimens (1.6%) showed both recipient and donor genome. These findings are the first report of donor DNA integration into a sturgeon embryo after interspecific cloning. In all, we provide evidence that cloning with the multiple donor somatic cells can be feasible in the future. Despite the fact that the sturgeon cloning faces limitations, to date it is the most promising technique for their preservation.Effrosyni FatiraMiloš HavelkaCatherine LabbéAlexandra DepincéMartin PšeničkaTaiju SaitoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Effrosyni Fatira Miloš Havelka Catherine Labbé Alexandra Depincé Martin Pšenička Taiju Saito A newly developed cloning technique in sturgeons; an important step towards recovering endangered species
description	Abstract Several steps of sturgeon somatic cell nuclear transfer (SCNT) have been recently established, but improvements are needed to make it a feasible tool to preserve the natural populations of this group of endangered species. The donor cell position inside the recipient egg seems to be crucial for its reprogramming; therefore by injecting multiple donor somatic cells instead of a single cell with a single manipulation, we increased the potential for embryo development. Using the Russian sturgeon Acipenser gueldenstaedtii as a multiple cell donor and sterlet Acipenser ruthenus as the non-enucleated egg recipient, we obtained higher proportion of eggs developing into embryos than previously reported with single-SCNT. Molecular data showed the production of a specimen (0.8%) contained only the donor genome with no contribution from the recipient, while two specimens (1.6%) showed both recipient and donor genome. These findings are the first report of donor DNA integration into a sturgeon embryo after interspecific cloning. In all, we provide evidence that cloning with the multiple donor somatic cells can be feasible in the future. Despite the fact that the sturgeon cloning faces limitations, to date it is the most promising technique for their preservation.
format	article
author	Effrosyni Fatira Miloš Havelka Catherine Labbé Alexandra Depincé Martin Pšenička Taiju Saito
author_facet	Effrosyni Fatira Miloš Havelka Catherine Labbé Alexandra Depincé Martin Pšenička Taiju Saito
author_sort	Effrosyni Fatira
title	A newly developed cloning technique in sturgeons; an important step towards recovering endangered species
title_short	A newly developed cloning technique in sturgeons; an important step towards recovering endangered species
title_full	A newly developed cloning technique in sturgeons; an important step towards recovering endangered species
title_fullStr	A newly developed cloning technique in sturgeons; an important step towards recovering endangered species
title_full_unstemmed	A newly developed cloning technique in sturgeons; an important step towards recovering endangered species
title_sort	newly developed cloning technique in sturgeons; an important step towards recovering endangered species
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/04402796dafb487eb4828353581b7bc3
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A newly developed cloning technique in sturgeons; an important step towards recovering endangered species

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