CRISPR Tackles Emerging Viral Pathogens

Understanding the dynamic relationship between viral pathogens and cellular host factors is critical to furthering our knowledge of viral replication, disease mechanisms and development of anti-viral therapeutics. CRISPR genome editing technology has enhanced this understanding, by allowing identifi...

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Autores principales:	Emily N. Kirby, Byron Shue, Paul Q. Thomas, Michael R. Beard
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	CRISPR KO CRISPRa coronavirus flavivirus SARS-CoV-2 genome editing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0448a9574aec439fa47a55d0b5cb1589
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spelling	oai:doaj.org-article:0448a9574aec439fa47a55d0b5cb15892021-11-25T19:12:54ZCRISPR Tackles Emerging Viral Pathogens10.3390/v131121571999-4915https://doaj.org/article/0448a9574aec439fa47a55d0b5cb15892021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2157https://doaj.org/toc/1999-4915Understanding the dynamic relationship between viral pathogens and cellular host factors is critical to furthering our knowledge of viral replication, disease mechanisms and development of anti-viral therapeutics. CRISPR genome editing technology has enhanced this understanding, by allowing identification of pro-viral and anti-viral cellular host factors for a wide range of viruses, most recently the cause of the COVID-19 pandemic, SARS-CoV-2. This review will discuss how CRISPR knockout and CRISPR activation genome-wide screening methods are a robust tool to investigate the viral life cycle and how other class 2 CRISPR systems are being repurposed for diagnostics.Emily N. KirbyByron ShuePaul Q. ThomasMichael R. BeardMDPI AGarticleCRISPR KOCRISPRacoronavirusflavivirusSARS-CoV-2genome editingMicrobiologyQR1-502ENViruses, Vol 13, Iss 2157, p 2157 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CRISPR KO CRISPRa coronavirus flavivirus SARS-CoV-2 genome editing Microbiology QR1-502
spellingShingle	CRISPR KO CRISPRa coronavirus flavivirus SARS-CoV-2 genome editing Microbiology QR1-502 Emily N. Kirby Byron Shue Paul Q. Thomas Michael R. Beard CRISPR Tackles Emerging Viral Pathogens
description	Understanding the dynamic relationship between viral pathogens and cellular host factors is critical to furthering our knowledge of viral replication, disease mechanisms and development of anti-viral therapeutics. CRISPR genome editing technology has enhanced this understanding, by allowing identification of pro-viral and anti-viral cellular host factors for a wide range of viruses, most recently the cause of the COVID-19 pandemic, SARS-CoV-2. This review will discuss how CRISPR knockout and CRISPR activation genome-wide screening methods are a robust tool to investigate the viral life cycle and how other class 2 CRISPR systems are being repurposed for diagnostics.
format	article
author	Emily N. Kirby Byron Shue Paul Q. Thomas Michael R. Beard
author_facet	Emily N. Kirby Byron Shue Paul Q. Thomas Michael R. Beard
author_sort	Emily N. Kirby
title	CRISPR Tackles Emerging Viral Pathogens
title_short	CRISPR Tackles Emerging Viral Pathogens
title_full	CRISPR Tackles Emerging Viral Pathogens
title_fullStr	CRISPR Tackles Emerging Viral Pathogens
title_full_unstemmed	CRISPR Tackles Emerging Viral Pathogens
title_sort	crispr tackles emerging viral pathogens
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0448a9574aec439fa47a55d0b5cb1589
work_keys_str_mv	AT emilynkirby crisprtacklesemergingviralpathogens AT byronshue crisprtacklesemergingviralpathogens AT paulqthomas crisprtacklesemergingviralpathogens AT michaelrbeard crisprtacklesemergingviralpathogens
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CRISPR Tackles Emerging Viral Pathogens

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