Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence

Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light sca...

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Autores principales: Zhihang Xiong, Hongjian Wang, Jiajin Li, Ran Liao, Haoji Mai, Caizhong Guan, Zhiming Guo, Shangpan Yang, Yan Chen, Biwang Liu, Tong Liu, Hongyi Li, Wenzheng Ding, Yaguang Zeng, Hui Ma
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Publicado: MDPI AG 2021
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Acceso en línea:https://doaj.org/article/0448b7c2bb0e4a6e8f19bf92da691eaf
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spelling oai:doaj.org-article:0448b7c2bb0e4a6e8f19bf92da691eaf2021-11-25T16:54:56ZProbing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence10.3390/bios111104162079-6374https://doaj.org/article/0448b7c2bb0e4a6e8f19bf92da691eaf2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/416https://doaj.org/toc/2079-6374Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and fluorescence, aiming to obtain an effective classification of microplastics and microalgae. Results show that the obtained classification accuracy is significantly higher than that for either of these two methods. The setup also successfully measures submicron particles and discriminates two species of <i>Synechococcus</i>. Our study demonstrates the feasibility of simultaneously measuring polarized light scattering and fluorescence, and the promising capability of our method for further aquatic environmental monitoring.Zhihang XiongHongjian WangJiajin LiRan LiaoHaoji MaiCaizhong GuanZhiming GuoShangpan YangYan ChenBiwang LiuTong LiuHongyi LiWenzheng DingYaguang ZengHui MaMDPI AGarticlepolarized light scatteringfluorescencesuspended particlesBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 416, p 416 (2021)
institution DOAJ
collection DOAJ
language EN
topic polarized light scattering
fluorescence
suspended particles
Biotechnology
TP248.13-248.65
spellingShingle polarized light scattering
fluorescence
suspended particles
Biotechnology
TP248.13-248.65
Zhihang Xiong
Hongjian Wang
Jiajin Li
Ran Liao
Haoji Mai
Caizhong Guan
Zhiming Guo
Shangpan Yang
Yan Chen
Biwang Liu
Tong Liu
Hongyi Li
Wenzheng Ding
Yaguang Zeng
Hui Ma
Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence
description Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and fluorescence, aiming to obtain an effective classification of microplastics and microalgae. Results show that the obtained classification accuracy is significantly higher than that for either of these two methods. The setup also successfully measures submicron particles and discriminates two species of <i>Synechococcus</i>. Our study demonstrates the feasibility of simultaneously measuring polarized light scattering and fluorescence, and the promising capability of our method for further aquatic environmental monitoring.
format article
author Zhihang Xiong
Hongjian Wang
Jiajin Li
Ran Liao
Haoji Mai
Caizhong Guan
Zhiming Guo
Shangpan Yang
Yan Chen
Biwang Liu
Tong Liu
Hongyi Li
Wenzheng Ding
Yaguang Zeng
Hui Ma
author_facet Zhihang Xiong
Hongjian Wang
Jiajin Li
Ran Liao
Haoji Mai
Caizhong Guan
Zhiming Guo
Shangpan Yang
Yan Chen
Biwang Liu
Tong Liu
Hongyi Li
Wenzheng Ding
Yaguang Zeng
Hui Ma
author_sort Zhihang Xiong
title Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence
title_short Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence
title_full Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence
title_fullStr Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence
title_full_unstemmed Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence
title_sort probing individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and fluorescence
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/0448b7c2bb0e4a6e8f19bf92da691eaf
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