Early staphylococcal biofilm formation on solid orthopaedic implant materials: in vitro study.

Biofilms forming on the surface of biomaterials can cause intractable implant-related infections. Bacterial adherence and early biofilm formation are influenced by the type of biomaterial used and the physical characteristics of implant surface. In this in vitro research, we evaluated the ability of...

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Autores principales:	Hironobu Koseki, Akihiko Yonekura, Takayuki Shida, Itaru Yoda, Hidehiko Horiuchi, Yoshitomo Morinaga, Katsunori Yanagihara, Hideyuki Sakoda, Makoto Osaki, Masato Tomita
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/044d176a920e40d0a953f32bb3f2fa52
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spelling	oai:doaj.org-article:044d176a920e40d0a953f32bb3f2fa522021-11-25T05:57:13ZEarly staphylococcal biofilm formation on solid orthopaedic implant materials: in vitro study.1932-620310.1371/journal.pone.0107588https://doaj.org/article/044d176a920e40d0a953f32bb3f2fa522014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107588https://doaj.org/toc/1932-6203Biofilms forming on the surface of biomaterials can cause intractable implant-related infections. Bacterial adherence and early biofilm formation are influenced by the type of biomaterial used and the physical characteristics of implant surface. In this in vitro research, we evaluated the ability of Staphylococcus epidermidis, the main pathogen in implant-related infections, to form biofilms on the surface of the solid orthopaedic biomaterials, oxidized zirconium-niobium alloy, cobalt-chromium-molybdenum alloy (Co-Cr-Mo), titanium alloy (Ti-6Al-4V), commercially pure titanium (cp-Ti) and stainless steel. A bacterial suspension of Staphylococcus epidermidis strain RP62A (ATCC35984) was added to the surface of specimens and incubated. The stained biofilms were imaged with a digital optical microscope and the biofilm coverage rate (BCR) was calculated. The total amount of biofilm was determined with the crystal violet assay and the number of viable cells in the biofilm was counted using the plate count method. The BCR of all the biomaterials rose in proportion to culture duration. After culturing for 2-4 hours, the BCR was similar for all materials. However, after culturing for 6 hours, the BCR for Co-Cr-Mo alloy was significantly lower than for Ti-6Al-4V, cp-Ti and stainless steel (P<0.05). The absorbance value determined in the crystal violet assay and the number of viable cells on Co-Cr-Mo were not significantly lower than for the other materials (P>0.05). These results suggest that surface properties, such as hydrophobicity or the low surface free energy of Co-Cr-Mo, may have some influence in inhibiting or delaying the two-dimensional expansion of biofilm on surfaces with a similar degree of smoothness.Hironobu KosekiAkihiko YonekuraTakayuki ShidaItaru YodaHidehiko HoriuchiYoshitomo MorinagaKatsunori YanagiharaHideyuki SakodaMakoto OsakiMasato TomitaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e107588 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hironobu Koseki Akihiko Yonekura Takayuki Shida Itaru Yoda Hidehiko Horiuchi Yoshitomo Morinaga Katsunori Yanagihara Hideyuki Sakoda Makoto Osaki Masato Tomita Early staphylococcal biofilm formation on solid orthopaedic implant materials: in vitro study.
description	Biofilms forming on the surface of biomaterials can cause intractable implant-related infections. Bacterial adherence and early biofilm formation are influenced by the type of biomaterial used and the physical characteristics of implant surface. In this in vitro research, we evaluated the ability of Staphylococcus epidermidis, the main pathogen in implant-related infections, to form biofilms on the surface of the solid orthopaedic biomaterials, oxidized zirconium-niobium alloy, cobalt-chromium-molybdenum alloy (Co-Cr-Mo), titanium alloy (Ti-6Al-4V), commercially pure titanium (cp-Ti) and stainless steel. A bacterial suspension of Staphylococcus epidermidis strain RP62A (ATCC35984) was added to the surface of specimens and incubated. The stained biofilms were imaged with a digital optical microscope and the biofilm coverage rate (BCR) was calculated. The total amount of biofilm was determined with the crystal violet assay and the number of viable cells in the biofilm was counted using the plate count method. The BCR of all the biomaterials rose in proportion to culture duration. After culturing for 2-4 hours, the BCR was similar for all materials. However, after culturing for 6 hours, the BCR for Co-Cr-Mo alloy was significantly lower than for Ti-6Al-4V, cp-Ti and stainless steel (P<0.05). The absorbance value determined in the crystal violet assay and the number of viable cells on Co-Cr-Mo were not significantly lower than for the other materials (P>0.05). These results suggest that surface properties, such as hydrophobicity or the low surface free energy of Co-Cr-Mo, may have some influence in inhibiting or delaying the two-dimensional expansion of biofilm on surfaces with a similar degree of smoothness.
format	article
author	Hironobu Koseki Akihiko Yonekura Takayuki Shida Itaru Yoda Hidehiko Horiuchi Yoshitomo Morinaga Katsunori Yanagihara Hideyuki Sakoda Makoto Osaki Masato Tomita
author_facet	Hironobu Koseki Akihiko Yonekura Takayuki Shida Itaru Yoda Hidehiko Horiuchi Yoshitomo Morinaga Katsunori Yanagihara Hideyuki Sakoda Makoto Osaki Masato Tomita
author_sort	Hironobu Koseki
title	Early staphylococcal biofilm formation on solid orthopaedic implant materials: in vitro study.
title_short	Early staphylococcal biofilm formation on solid orthopaedic implant materials: in vitro study.
title_full	Early staphylococcal biofilm formation on solid orthopaedic implant materials: in vitro study.
title_fullStr	Early staphylococcal biofilm formation on solid orthopaedic implant materials: in vitro study.
title_full_unstemmed	Early staphylococcal biofilm formation on solid orthopaedic implant materials: in vitro study.
title_sort	early staphylococcal biofilm formation on solid orthopaedic implant materials: in vitro study.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/044d176a920e40d0a953f32bb3f2fa52
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Early staphylococcal biofilm formation on solid orthopaedic implant materials: in vitro study.

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