Long-Range Cooperative Disassembly and Aging During Adenovirus Uncoating

Icosahedral virus capsids are closed shells built up with a hexagonal lattice of proteins, which incorporate pentamers at their fivefold vertices. Human adenovirus particles lose pentamers (pentons) during infection under a variety of physicochemical cues, including mechanical pulling of molecular m...

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Autores principales:	Natalia Martín-González, Pablo Ibáñez-Freire, Álvaro Ortega-Esteban, Mara Laguna-Castro, Carmen San Martín, Alejandro Valbuena, Rafael Delgado-Buscalioni, Pedro J. de Pablo
Formato:	article
Lenguaje:	EN
Publicado:	American Physical Society 2021
Materias:	Physics QC1-999
Acceso en línea:	https://doaj.org/article/0450304d917a4b97b8719ae17be46390
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spelling	oai:doaj.org-article:0450304d917a4b97b8719ae17be463902021-12-02T14:34:57ZLong-Range Cooperative Disassembly and Aging During Adenovirus Uncoating10.1103/PhysRevX.11.0210252160-3308https://doaj.org/article/0450304d917a4b97b8719ae17be463902021-04-01T00:00:00Zhttp://doi.org/10.1103/PhysRevX.11.021025http://doi.org/10.1103/PhysRevX.11.021025https://doaj.org/toc/2160-3308Icosahedral virus capsids are closed shells built up with a hexagonal lattice of proteins, which incorporate pentamers at their fivefold vertices. Human adenovirus particles lose pentamers (pentons) during infection under a variety of physicochemical cues, including mechanical pulling of molecular motors and the viscous drag of the cytoplasm. By combining atomic force microscopy experiments with survival analysis and Markovian transition state theory, we investigate the sequence of adenovirus penton disassembly that reveals the aging of the virus structure. We show evidence that the lifetime of pentons gradually decreases, accompanied by capsid softening as neighboring pentons are lost. This cooperative dismantling process, which involves first-neighbor penton-penton distances of at least 45 nm, leads to a 50% increase in the virus disassembling rate of the virus particle. Theory and experiments fit remarkably well, allowing us to obtain the spontaneous escape rate and the energy barrier of penton disassembly (∼30 k_{B}T). The observed increase in the penton’s loss rate reveals long-range structural correlations within the capsid. Our estimations suggest that the mechanical cues arising from the strokes of protein motors carrying the virus to the nucleus could help penton disassembly and warrant the timely delivery of weak-enough capsids for adenovirus infection.Natalia Martín-GonzálezPablo Ibáñez-FreireÁlvaro Ortega-EstebanMara Laguna-CastroCarmen San MartínAlejandro ValbuenaRafael Delgado-BuscalioniPedro J. de PabloAmerican Physical SocietyarticlePhysicsQC1-999ENPhysical Review X, Vol 11, Iss 2, p 021025 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Physics QC1-999
spellingShingle	Physics QC1-999 Natalia Martín-González Pablo Ibáñez-Freire Álvaro Ortega-Esteban Mara Laguna-Castro Carmen San Martín Alejandro Valbuena Rafael Delgado-Buscalioni Pedro J. de Pablo Long-Range Cooperative Disassembly and Aging During Adenovirus Uncoating
description	Icosahedral virus capsids are closed shells built up with a hexagonal lattice of proteins, which incorporate pentamers at their fivefold vertices. Human adenovirus particles lose pentamers (pentons) during infection under a variety of physicochemical cues, including mechanical pulling of molecular motors and the viscous drag of the cytoplasm. By combining atomic force microscopy experiments with survival analysis and Markovian transition state theory, we investigate the sequence of adenovirus penton disassembly that reveals the aging of the virus structure. We show evidence that the lifetime of pentons gradually decreases, accompanied by capsid softening as neighboring pentons are lost. This cooperative dismantling process, which involves first-neighbor penton-penton distances of at least 45 nm, leads to a 50% increase in the virus disassembling rate of the virus particle. Theory and experiments fit remarkably well, allowing us to obtain the spontaneous escape rate and the energy barrier of penton disassembly (∼30 k_{B}T). The observed increase in the penton’s loss rate reveals long-range structural correlations within the capsid. Our estimations suggest that the mechanical cues arising from the strokes of protein motors carrying the virus to the nucleus could help penton disassembly and warrant the timely delivery of weak-enough capsids for adenovirus infection.
format	article
author	Natalia Martín-González Pablo Ibáñez-Freire Álvaro Ortega-Esteban Mara Laguna-Castro Carmen San Martín Alejandro Valbuena Rafael Delgado-Buscalioni Pedro J. de Pablo
author_facet	Natalia Martín-González Pablo Ibáñez-Freire Álvaro Ortega-Esteban Mara Laguna-Castro Carmen San Martín Alejandro Valbuena Rafael Delgado-Buscalioni Pedro J. de Pablo
author_sort	Natalia Martín-González
title	Long-Range Cooperative Disassembly and Aging During Adenovirus Uncoating
title_short	Long-Range Cooperative Disassembly and Aging During Adenovirus Uncoating
title_full	Long-Range Cooperative Disassembly and Aging During Adenovirus Uncoating
title_fullStr	Long-Range Cooperative Disassembly and Aging During Adenovirus Uncoating
title_full_unstemmed	Long-Range Cooperative Disassembly and Aging During Adenovirus Uncoating
title_sort	long-range cooperative disassembly and aging during adenovirus uncoating
publisher	American Physical Society
publishDate	2021
url	https://doaj.org/article/0450304d917a4b97b8719ae17be46390
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Long-Range Cooperative Disassembly and Aging During Adenovirus Uncoating

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