Stage-specific histone modification profiles reveal global transitions in the Xenopus embryonic epigenome.

Vertebrate embryos are derived from a transitory pool of pluripotent cells. By the process of embryonic induction, these precursor cells are assigned to specific fates and differentiation programs. Histone post-translational modifications are thought to play a key role in the establishment and maint...

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Autores principales:	Tobias D Schneider, Jose M Arteaga-Salas, Edith Mentele, Robert David, Dario Nicetto, Axel Imhof, Ralph A W Rupp
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0451cf1920a24b0693466c3569dd104f
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spelling	oai:doaj.org-article:0451cf1920a24b0693466c3569dd104f2021-11-18T06:49:33ZStage-specific histone modification profiles reveal global transitions in the Xenopus embryonic epigenome.1932-620310.1371/journal.pone.0022548https://doaj.org/article/0451cf1920a24b0693466c3569dd104f2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21814581/?tool=EBIhttps://doaj.org/toc/1932-6203Vertebrate embryos are derived from a transitory pool of pluripotent cells. By the process of embryonic induction, these precursor cells are assigned to specific fates and differentiation programs. Histone post-translational modifications are thought to play a key role in the establishment and maintenance of stable gene expression patterns underlying these processes. While on gene level histone modifications are known to change during differentiation, very little is known about the quantitative fluctuations in bulk histone modifications during development. To investigate this issue we analysed histones isolated from four different developmental stages of Xenopus laevis by mass spectrometry. In toto, we quantified 59 modification states on core histones H3 and H4 from blastula to tadpole stages. During this developmental period, we observed in general an increase in the unmodified states, and a shift from histone modifications associated with transcriptional activity to transcriptionally repressive histone marks. We also compared these naturally occurring patterns with the histone modifications of murine ES cells, detecting large differences in the methylation patterns of histone H3 lysines 27 and 36 between pluripotent ES cells and pluripotent cells from Xenopus blastulae. By combining all detected modification transitions we could cluster their patterns according to their embryonic origin, defining specific histone modification profiles (HMPs) for each developmental stage. To our knowledge, this data set represents the first compendium of covalent histone modifications and their quantitative flux during normogenesis in a vertebrate model organism. The HMPs indicate a stepwise maturation of the embryonic epigenome, which may be causal to the progressing restriction of cellular potency during development.Tobias D SchneiderJose M Arteaga-SalasEdith MenteleRobert DavidDario NicettoAxel ImhofRalph A W RuppPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22548 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tobias D Schneider Jose M Arteaga-Salas Edith Mentele Robert David Dario Nicetto Axel Imhof Ralph A W Rupp Stage-specific histone modification profiles reveal global transitions in the Xenopus embryonic epigenome.
description	Vertebrate embryos are derived from a transitory pool of pluripotent cells. By the process of embryonic induction, these precursor cells are assigned to specific fates and differentiation programs. Histone post-translational modifications are thought to play a key role in the establishment and maintenance of stable gene expression patterns underlying these processes. While on gene level histone modifications are known to change during differentiation, very little is known about the quantitative fluctuations in bulk histone modifications during development. To investigate this issue we analysed histones isolated from four different developmental stages of Xenopus laevis by mass spectrometry. In toto, we quantified 59 modification states on core histones H3 and H4 from blastula to tadpole stages. During this developmental period, we observed in general an increase in the unmodified states, and a shift from histone modifications associated with transcriptional activity to transcriptionally repressive histone marks. We also compared these naturally occurring patterns with the histone modifications of murine ES cells, detecting large differences in the methylation patterns of histone H3 lysines 27 and 36 between pluripotent ES cells and pluripotent cells from Xenopus blastulae. By combining all detected modification transitions we could cluster their patterns according to their embryonic origin, defining specific histone modification profiles (HMPs) for each developmental stage. To our knowledge, this data set represents the first compendium of covalent histone modifications and their quantitative flux during normogenesis in a vertebrate model organism. The HMPs indicate a stepwise maturation of the embryonic epigenome, which may be causal to the progressing restriction of cellular potency during development.
format	article
author	Tobias D Schneider Jose M Arteaga-Salas Edith Mentele Robert David Dario Nicetto Axel Imhof Ralph A W Rupp
author_facet	Tobias D Schneider Jose M Arteaga-Salas Edith Mentele Robert David Dario Nicetto Axel Imhof Ralph A W Rupp
author_sort	Tobias D Schneider
title	Stage-specific histone modification profiles reveal global transitions in the Xenopus embryonic epigenome.
title_short	Stage-specific histone modification profiles reveal global transitions in the Xenopus embryonic epigenome.
title_full	Stage-specific histone modification profiles reveal global transitions in the Xenopus embryonic epigenome.
title_fullStr	Stage-specific histone modification profiles reveal global transitions in the Xenopus embryonic epigenome.
title_full_unstemmed	Stage-specific histone modification profiles reveal global transitions in the Xenopus embryonic epigenome.
title_sort	stage-specific histone modification profiles reveal global transitions in the xenopus embryonic epigenome.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/0451cf1920a24b0693466c3569dd104f
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Stage-specific histone modification profiles reveal global transitions in the Xenopus embryonic epigenome.

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