Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters

Abstract Dyslipidemia is a documented risk factor for cardiovascular diseases and other metabolic disorders. Therefore, the analysis of hyperlipidemia (HL)-related miRNAs is a potential approach for achieving new prognostic markers in lipid-metabolism related diseases. We aimed to analyze specific d...

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Autores principales:	Teodora Barbalata, Lu Zhang, Madalina D. Dulceanu, Camelia S. Stancu, Yvan Devaux, Anca V. Sima, Loredan S. Niculescu, EU-CardioRNA COST Action CA17129
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/04563b7b9fed40e18e78f234434b1448
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spelling	oai:doaj.org-article:04563b7b9fed40e18e78f234434b14482021-12-02T12:33:44ZRegulation of microRNAs in high-fat diet induced hyperlipidemic hamsters10.1038/s41598-020-77539-42045-2322https://doaj.org/article/04563b7b9fed40e18e78f234434b14482020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77539-4https://doaj.org/toc/2045-2322Abstract Dyslipidemia is a documented risk factor for cardiovascular diseases and other metabolic disorders. Therefore, the analysis of hyperlipidemia (HL)-related miRNAs is a potential approach for achieving new prognostic markers in lipid-metabolism related diseases. We aimed to analyze specific distribution of miRNAs in different tissues from HL animals. Golden Syrian hamsters were fed either regular chow (NL) or high-fat diet (HL) for 12 weeks. Microarray miRNAs profiling was performed in liver, heart and small intestine and data analyzed by R-studio software. Functional enrichment bioinformatics analysis was performed using miRWalk and DAVID tools. We observed a dysregulation of miRNAs in HL tissues evidencing a discrete distribution in the heart-liver axis and three lipid metabolism-related miRNAs were identified: hsa-miR-223-3p, hsa-miR-21-5p, and hsa-miR-146a-5p. Expression levels of these miRNAs were increased in HL livers and hearts. Functional bioinformatics analysis showed involvement of these miRNAs in the regulation of biological processes altered in HL conditions such as lipid metabolic process, fat cell differentiation, regulation of smooth muscle cells and cardiac septum development. We identified a set of miRNAs dysregulated in different tissues of HFD-induced HL hamsters. These findings motivate further studies aiming to investigate novel molecular mechanisms of lipid metabolism and atherogenic HL.Teodora BarbalataLu ZhangMadalina D. DulceanuCamelia S. StancuYvan DevauxAnca V. SimaLoredan S. NiculescuEU-CardioRNA COST Action CA17129Nature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Teodora Barbalata Lu Zhang Madalina D. Dulceanu Camelia S. Stancu Yvan Devaux Anca V. Sima Loredan S. Niculescu EU-CardioRNA COST Action CA17129 Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters
description	Abstract Dyslipidemia is a documented risk factor for cardiovascular diseases and other metabolic disorders. Therefore, the analysis of hyperlipidemia (HL)-related miRNAs is a potential approach for achieving new prognostic markers in lipid-metabolism related diseases. We aimed to analyze specific distribution of miRNAs in different tissues from HL animals. Golden Syrian hamsters were fed either regular chow (NL) or high-fat diet (HL) for 12 weeks. Microarray miRNAs profiling was performed in liver, heart and small intestine and data analyzed by R-studio software. Functional enrichment bioinformatics analysis was performed using miRWalk and DAVID tools. We observed a dysregulation of miRNAs in HL tissues evidencing a discrete distribution in the heart-liver axis and three lipid metabolism-related miRNAs were identified: hsa-miR-223-3p, hsa-miR-21-5p, and hsa-miR-146a-5p. Expression levels of these miRNAs were increased in HL livers and hearts. Functional bioinformatics analysis showed involvement of these miRNAs in the regulation of biological processes altered in HL conditions such as lipid metabolic process, fat cell differentiation, regulation of smooth muscle cells and cardiac septum development. We identified a set of miRNAs dysregulated in different tissues of HFD-induced HL hamsters. These findings motivate further studies aiming to investigate novel molecular mechanisms of lipid metabolism and atherogenic HL.
format	article
author	Teodora Barbalata Lu Zhang Madalina D. Dulceanu Camelia S. Stancu Yvan Devaux Anca V. Sima Loredan S. Niculescu EU-CardioRNA COST Action CA17129
author_facet	Teodora Barbalata Lu Zhang Madalina D. Dulceanu Camelia S. Stancu Yvan Devaux Anca V. Sima Loredan S. Niculescu EU-CardioRNA COST Action CA17129
author_sort	Teodora Barbalata
title	Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters
title_short	Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters
title_full	Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters
title_fullStr	Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters
title_full_unstemmed	Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters
title_sort	regulation of micrornas in high-fat diet induced hyperlipidemic hamsters
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/04563b7b9fed40e18e78f234434b1448
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Regulation of microRNAs in high-fat diet induced hyperlipidemic hamsters

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