Evolutionarily conserved BIL4 suppresses the degradation of brassinosteroid receptor BRI1 and regulates cell elongation

Abstract Brassinosteroids (BRs), plant steroid hormones, play important roles in plant cell elongation and differentiation. To investigate the mechanisms of BR signaling, we previously used the BR biosynthesis inhibitor Brz as a chemical biology tool and identified the Brz-insensitive-long hypocotyl...

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Autores principales:	Ayumi Yamagami, Chieko Saito, Miki Nakazawa, Shozo Fujioka, Tomohiro Uemura, Minami Matsui, Masaaki Sakuta, Kazuo Shinozaki, Hiroyuki Osada, Akihiko Nakano, Tadao Asami, Takeshi Nakano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0464ed82eb694adc87d1f063eb986632
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spelling	oai:doaj.org-article:0464ed82eb694adc87d1f063eb9866322021-12-02T15:05:42ZEvolutionarily conserved BIL4 suppresses the degradation of brassinosteroid receptor BRI1 and regulates cell elongation10.1038/s41598-017-06016-22045-2322https://doaj.org/article/0464ed82eb694adc87d1f063eb9866322017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06016-2https://doaj.org/toc/2045-2322Abstract Brassinosteroids (BRs), plant steroid hormones, play important roles in plant cell elongation and differentiation. To investigate the mechanisms of BR signaling, we previously used the BR biosynthesis inhibitor Brz as a chemical biology tool and identified the Brz-insensitive-long hypocotyl4 mutant (bil4). Although the BIL4 gene encodes a seven-transmembrane-domain protein that is evolutionarily conserved in plants and animals, the molecular function of BIL4 in BR signaling has not been elucidated. Here, we demonstrate that BIL4 is expressed in early elongating cells and regulates cell elongation in Arabidopsis. BIL4 also activates BR signaling and interacts with the BR receptor brassinosteroid insensitive 1 (BRI1) in endosomes. BIL4 deficiency increases the localization of BRI1 in the vacuoles. Our results demonstrate that BIL4 regulates cell elongation and BR signaling via the regulation of BRI1 localization.Ayumi YamagamiChieko SaitoMiki NakazawaShozo FujiokaTomohiro UemuraMinami MatsuiMasaaki SakutaKazuo ShinozakiHiroyuki OsadaAkihiko NakanoTadao AsamiTakeshi NakanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ayumi Yamagami Chieko Saito Miki Nakazawa Shozo Fujioka Tomohiro Uemura Minami Matsui Masaaki Sakuta Kazuo Shinozaki Hiroyuki Osada Akihiko Nakano Tadao Asami Takeshi Nakano Evolutionarily conserved BIL4 suppresses the degradation of brassinosteroid receptor BRI1 and regulates cell elongation
description	Abstract Brassinosteroids (BRs), plant steroid hormones, play important roles in plant cell elongation and differentiation. To investigate the mechanisms of BR signaling, we previously used the BR biosynthesis inhibitor Brz as a chemical biology tool and identified the Brz-insensitive-long hypocotyl4 mutant (bil4). Although the BIL4 gene encodes a seven-transmembrane-domain protein that is evolutionarily conserved in plants and animals, the molecular function of BIL4 in BR signaling has not been elucidated. Here, we demonstrate that BIL4 is expressed in early elongating cells and regulates cell elongation in Arabidopsis. BIL4 also activates BR signaling and interacts with the BR receptor brassinosteroid insensitive 1 (BRI1) in endosomes. BIL4 deficiency increases the localization of BRI1 in the vacuoles. Our results demonstrate that BIL4 regulates cell elongation and BR signaling via the regulation of BRI1 localization.
format	article
author	Ayumi Yamagami Chieko Saito Miki Nakazawa Shozo Fujioka Tomohiro Uemura Minami Matsui Masaaki Sakuta Kazuo Shinozaki Hiroyuki Osada Akihiko Nakano Tadao Asami Takeshi Nakano
author_facet	Ayumi Yamagami Chieko Saito Miki Nakazawa Shozo Fujioka Tomohiro Uemura Minami Matsui Masaaki Sakuta Kazuo Shinozaki Hiroyuki Osada Akihiko Nakano Tadao Asami Takeshi Nakano
author_sort	Ayumi Yamagami
title	Evolutionarily conserved BIL4 suppresses the degradation of brassinosteroid receptor BRI1 and regulates cell elongation
title_short	Evolutionarily conserved BIL4 suppresses the degradation of brassinosteroid receptor BRI1 and regulates cell elongation
title_full	Evolutionarily conserved BIL4 suppresses the degradation of brassinosteroid receptor BRI1 and regulates cell elongation
title_fullStr	Evolutionarily conserved BIL4 suppresses the degradation of brassinosteroid receptor BRI1 and regulates cell elongation
title_full_unstemmed	Evolutionarily conserved BIL4 suppresses the degradation of brassinosteroid receptor BRI1 and regulates cell elongation
title_sort	evolutionarily conserved bil4 suppresses the degradation of brassinosteroid receptor bri1 and regulates cell elongation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0464ed82eb694adc87d1f063eb986632
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Evolutionarily conserved BIL4 suppresses the degradation of brassinosteroid receptor BRI1 and regulates cell elongation

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