Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking

Background: Immune cells show distinct motion patterns that change upon inflammatory stimuli. Monocytes patrol the vasculature to screen for pathogens, thereby exerting an early task of innate immunity. Here, we aimed to non-invasively analyse single patrolling monocyte behaviour upon inflammatory s...

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Autores principales:	Max Masthoff, Felix Noah Freppon, Lisa Zondler, Enrica Wilken, Lydia Wachsmuth, Silke Niemann, Christian Schwarz, Ina Fredrich, Asli Havlas, Helena Block, Mirjam Gerwing, Anne Helfen, Walter Heindel, Alexander Zarbock, Moritz Wildgruber, Cornelius Faber
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	patrolling monocytes time-lapse MRI single cell tracking immune response iron oxide nanoparticles Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/04693da21a3d46ce8204d1a900639621
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spelling	oai:doaj.org-article:04693da21a3d46ce8204d1a9006396212021-11-06T04:31:19ZResolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking2352-396410.1016/j.ebiom.2021.103670https://doaj.org/article/04693da21a3d46ce8204d1a9006396212021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352396421004643https://doaj.org/toc/2352-3964Background: Immune cells show distinct motion patterns that change upon inflammatory stimuli. Monocytes patrol the vasculature to screen for pathogens, thereby exerting an early task of innate immunity. Here, we aimed to non-invasively analyse single patrolling monocyte behaviour upon inflammatory stimuli. Methods: We used time-lapse Magnetic Resonance Imaging (MRI) of the murine brain to dynamically track single patrolling monocytes within the circulation distant to the actual site of inflammation in different inflammatory conditions, ranging from a subcutaneous pellet model to severe peritonitis and bacteraemia. Findings: Single patrolling immune cells with a velocity of <1 µm/s could be detected and followed dynamically using time-lapse MRI. We show, that due to local and systemic stimuli the slowly patrolling behaviour of monocytes is altered systemically and differs with type, duration and strength of the underlying stimulus. Interpretation: Using time-lapse MRI, it is now possible to investigate the behaviour of single circulating monocytes over the course of the systemic immune response. Monocyte patrolling behaviour is altered systemically even before the onset of clinical symptoms distant to and depending on the underlying inflammatory stimulus. Funding: This study was supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - CRC 1009 - 194468054 to AZ, CF and - CRC 1450 - 431460824 to MM, SN, HB, AZ, CF, the Joachim Herz Foundation (Add-on Fellowship for Interdisciplinary Life Sciences to MM), the Interdisciplinary Centre for Clinical Research (IZKF, core unit PIX) and the Medical Faculty of the University of Muenster (MEDK fellowship to FF and IF).Max MasthoffFelix Noah FrepponLisa ZondlerEnrica WilkenLydia WachsmuthSilke NiemannChristian SchwarzIna FredrichAsli HavlasHelena BlockMirjam GerwingAnne HelfenWalter HeindelAlexander ZarbockMoritz WildgruberCornelius FaberElsevierarticlepatrolling monocytestime-lapse MRIsingle cell trackingimmune responseiron oxide nanoparticlesMedicineRMedicine (General)R5-920ENEBioMedicine, Vol 73, Iss , Pp 103670- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	patrolling monocytes time-lapse MRI single cell tracking immune response iron oxide nanoparticles Medicine R Medicine (General) R5-920
spellingShingle	patrolling monocytes time-lapse MRI single cell tracking immune response iron oxide nanoparticles Medicine R Medicine (General) R5-920 Max Masthoff Felix Noah Freppon Lisa Zondler Enrica Wilken Lydia Wachsmuth Silke Niemann Christian Schwarz Ina Fredrich Asli Havlas Helena Block Mirjam Gerwing Anne Helfen Walter Heindel Alexander Zarbock Moritz Wildgruber Cornelius Faber Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking
description	Background: Immune cells show distinct motion patterns that change upon inflammatory stimuli. Monocytes patrol the vasculature to screen for pathogens, thereby exerting an early task of innate immunity. Here, we aimed to non-invasively analyse single patrolling monocyte behaviour upon inflammatory stimuli. Methods: We used time-lapse Magnetic Resonance Imaging (MRI) of the murine brain to dynamically track single patrolling monocytes within the circulation distant to the actual site of inflammation in different inflammatory conditions, ranging from a subcutaneous pellet model to severe peritonitis and bacteraemia. Findings: Single patrolling immune cells with a velocity of <1 µm/s could be detected and followed dynamically using time-lapse MRI. We show, that due to local and systemic stimuli the slowly patrolling behaviour of monocytes is altered systemically and differs with type, duration and strength of the underlying stimulus. Interpretation: Using time-lapse MRI, it is now possible to investigate the behaviour of single circulating monocytes over the course of the systemic immune response. Monocyte patrolling behaviour is altered systemically even before the onset of clinical symptoms distant to and depending on the underlying inflammatory stimulus. Funding: This study was supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - CRC 1009 - 194468054 to AZ, CF and - CRC 1450 - 431460824 to MM, SN, HB, AZ, CF, the Joachim Herz Foundation (Add-on Fellowship for Interdisciplinary Life Sciences to MM), the Interdisciplinary Centre for Clinical Research (IZKF, core unit PIX) and the Medical Faculty of the University of Muenster (MEDK fellowship to FF and IF).
format	article
author	Max Masthoff Felix Noah Freppon Lisa Zondler Enrica Wilken Lydia Wachsmuth Silke Niemann Christian Schwarz Ina Fredrich Asli Havlas Helena Block Mirjam Gerwing Anne Helfen Walter Heindel Alexander Zarbock Moritz Wildgruber Cornelius Faber
author_facet	Max Masthoff Felix Noah Freppon Lisa Zondler Enrica Wilken Lydia Wachsmuth Silke Niemann Christian Schwarz Ina Fredrich Asli Havlas Helena Block Mirjam Gerwing Anne Helfen Walter Heindel Alexander Zarbock Moritz Wildgruber Cornelius Faber
author_sort	Max Masthoff
title	Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking
title_short	Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking
title_full	Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking
title_fullStr	Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking
title_full_unstemmed	Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking
title_sort	resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/04693da21a3d46ce8204d1a900639621
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Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking

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