Improving the sexual activity and reproduction of female zebrafish with high testosterone levels

Abstract High levels of testosterone cause clinical symptoms in female reproduction and possibly, alterations in sexuality. Yet, the underlying mechanisms remain to be examined. Here, we report a study that investigates the effects of testosterone in follicle development and sexual mating using zebr...

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Autores principales:	Congcong Liu, Sophie Yue, Joshua Solarz, Jessica Lee, Lei Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/046b18a0a9274858935e97a466f1bd33
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spelling	oai:doaj.org-article:046b18a0a9274858935e97a466f1bd332021-12-02T12:11:17ZImproving the sexual activity and reproduction of female zebrafish with high testosterone levels10.1038/s41598-021-83085-42045-2322https://doaj.org/article/046b18a0a9274858935e97a466f1bd332021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83085-4https://doaj.org/toc/2045-2322Abstract High levels of testosterone cause clinical symptoms in female reproduction and possibly, alterations in sexuality. Yet, the underlying mechanisms remain to be examined. Here, we report a study that investigates the effects of testosterone in follicle development and sexual mating using zebrafish models. We developed an acute zebrafish model with high testosterone levels by exposing young female zebrafish to testosterone dissolved in swimming water. After given a high concentration of testosterone treatment (e.g., 100 ng/ml), the fish showed hallmark pathological symptoms similar to those displayed in patients with polycystic ovary syndrome (PCOS), such as follicular growth-arrest, rare ovulation, ovary enlargement, decrease in reproduction, and down regulation of the expression of some PCOS susceptible genes, such as Tox3. These fish are referred to as the PCOS fish. By monitoring mating-like swimming behaviors, we measured the sexual activity of PCOS zebrafish. In general, the PCOS fish showed no desire to interact with males. As a consequence, their mating rate was decreased as compared to control animals. The sexuality levels of PCOS fish, however, could be improved after short periods of rearing in conditions that lack of males. After only 3 days of rearing alone, the PCOS fish showed an increase in sexuality levels and displayed characteristic swimming patterns for mating. After 30 days of separation from males, not only the sexual activity, but also the mating rate was improved in the PCOS fish. Together, the data suggests that zebrafish can serve as a new type of research model to further develop strategies for the treatment of reproductive disorders, such as those related to PCOS.Congcong LiuSophie YueJoshua SolarzJessica LeeLei LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Congcong Liu Sophie Yue Joshua Solarz Jessica Lee Lei Li Improving the sexual activity and reproduction of female zebrafish with high testosterone levels
description	Abstract High levels of testosterone cause clinical symptoms in female reproduction and possibly, alterations in sexuality. Yet, the underlying mechanisms remain to be examined. Here, we report a study that investigates the effects of testosterone in follicle development and sexual mating using zebrafish models. We developed an acute zebrafish model with high testosterone levels by exposing young female zebrafish to testosterone dissolved in swimming water. After given a high concentration of testosterone treatment (e.g., 100 ng/ml), the fish showed hallmark pathological symptoms similar to those displayed in patients with polycystic ovary syndrome (PCOS), such as follicular growth-arrest, rare ovulation, ovary enlargement, decrease in reproduction, and down regulation of the expression of some PCOS susceptible genes, such as Tox3. These fish are referred to as the PCOS fish. By monitoring mating-like swimming behaviors, we measured the sexual activity of PCOS zebrafish. In general, the PCOS fish showed no desire to interact with males. As a consequence, their mating rate was decreased as compared to control animals. The sexuality levels of PCOS fish, however, could be improved after short periods of rearing in conditions that lack of males. After only 3 days of rearing alone, the PCOS fish showed an increase in sexuality levels and displayed characteristic swimming patterns for mating. After 30 days of separation from males, not only the sexual activity, but also the mating rate was improved in the PCOS fish. Together, the data suggests that zebrafish can serve as a new type of research model to further develop strategies for the treatment of reproductive disorders, such as those related to PCOS.
format	article
author	Congcong Liu Sophie Yue Joshua Solarz Jessica Lee Lei Li
author_facet	Congcong Liu Sophie Yue Joshua Solarz Jessica Lee Lei Li
author_sort	Congcong Liu
title	Improving the sexual activity and reproduction of female zebrafish with high testosterone levels
title_short	Improving the sexual activity and reproduction of female zebrafish with high testosterone levels
title_full	Improving the sexual activity and reproduction of female zebrafish with high testosterone levels
title_fullStr	Improving the sexual activity and reproduction of female zebrafish with high testosterone levels
title_full_unstemmed	Improving the sexual activity and reproduction of female zebrafish with high testosterone levels
title_sort	improving the sexual activity and reproduction of female zebrafish with high testosterone levels
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/046b18a0a9274858935e97a466f1bd33
work_keys_str_mv	AT congcongliu improvingthesexualactivityandreproductionoffemalezebrafishwithhightestosteronelevels AT sophieyue improvingthesexualactivityandreproductionoffemalezebrafishwithhightestosteronelevels AT joshuasolarz improvingthesexualactivityandreproductionoffemalezebrafishwithhightestosteronelevels AT jessicalee improvingthesexualactivityandreproductionoffemalezebrafishwithhightestosteronelevels AT leili improvingthesexualactivityandreproductionoffemalezebrafishwithhightestosteronelevels
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Improving the sexual activity and reproduction of female zebrafish with high testosterone levels

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