Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>)

Feline herpesvirus type 1 (FHV-1) is endemic in captive cheetahs and sporadically causes devastating disease. Modified live vaccines (MLV), intended for use in domestic cats, are used in some captive cheetah populations and have been anecdotally linked to disease in certain subpopulations. Ten FHV-1...

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Autores principales:	Morgan E. Marino, Melanie A. Mironovich, Nikole E. Ineck, Scott B. Citino, Jessica A. Emerson, David J. Maggs, Lyndon M. Coghill, Edward J. Dubovi, Rachel C. Turner, Renee T. Carter, Andrew C. Lewin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	feline herpesvirus herpes Varicellovirus cheetah genome sequencing phylogenomic Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0477551e34924dbcbf0ea52b409f2c3d
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spelling	oai:doaj.org-article:0477551e34924dbcbf0ea52b409f2c3d2021-11-25T19:14:26ZFull Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>)10.3390/v131123071999-4915https://doaj.org/article/0477551e34924dbcbf0ea52b409f2c3d2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2307https://doaj.org/toc/1999-4915Feline herpesvirus type 1 (FHV-1) is endemic in captive cheetahs and sporadically causes devastating disease. Modified live vaccines (MLV), intended for use in domestic cats, are used in some captive cheetah populations and have been anecdotally linked to disease in certain subpopulations. Ten FHV-1 isolates from ten captive cheetahs and one isolate from an MLV used to inoculate four of the host animals were analyzed. Viral DNA was extracted for full-genome sequencing by Illumina MiSeq with viral genomes then used for phylogenomic and recombinational analyses. The FHV-1 shed by vaccinated cheetahs were almost identical to the MLV, with few variants among viral genomes. Eight cheetah FHV-1 isolates and the MLV were grouped in a clade along with FHV-1 isolates from domestic cats in the USA. The remaining two cheetah FHV-1 isolates (unknown host vaccine status) were not associated with a clade. The likely ancestral origin of these two isolates involves recombination events between Australian domestic cat and cheetah FHV-1 isolates. Collectively, these data suggest that the MLV is capable of causing clinical disease and viral shedding in some cheetahs and represents evidence of interspecies transmission of virus between domestic and wild cats.Morgan E. MarinoMelanie A. MironovichNikole E. IneckScott B. CitinoJessica A. EmersonDavid J. MaggsLyndon M. CoghillEdward J. DuboviRachel C. TurnerRenee T. CarterAndrew C. LewinMDPI AGarticlefeline herpesvirusherpesVaricelloviruscheetahgenome sequencingphylogenomicMicrobiologyQR1-502ENViruses, Vol 13, Iss 2307, p 2307 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	feline herpesvirus herpes Varicellovirus cheetah genome sequencing phylogenomic Microbiology QR1-502
spellingShingle	feline herpesvirus herpes Varicellovirus cheetah genome sequencing phylogenomic Microbiology QR1-502 Morgan E. Marino Melanie A. Mironovich Nikole E. Ineck Scott B. Citino Jessica A. Emerson David J. Maggs Lyndon M. Coghill Edward J. Dubovi Rachel C. Turner Renee T. Carter Andrew C. Lewin Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>)
description	Feline herpesvirus type 1 (FHV-1) is endemic in captive cheetahs and sporadically causes devastating disease. Modified live vaccines (MLV), intended for use in domestic cats, are used in some captive cheetah populations and have been anecdotally linked to disease in certain subpopulations. Ten FHV-1 isolates from ten captive cheetahs and one isolate from an MLV used to inoculate four of the host animals were analyzed. Viral DNA was extracted for full-genome sequencing by Illumina MiSeq with viral genomes then used for phylogenomic and recombinational analyses. The FHV-1 shed by vaccinated cheetahs were almost identical to the MLV, with few variants among viral genomes. Eight cheetah FHV-1 isolates and the MLV were grouped in a clade along with FHV-1 isolates from domestic cats in the USA. The remaining two cheetah FHV-1 isolates (unknown host vaccine status) were not associated with a clade. The likely ancestral origin of these two isolates involves recombination events between Australian domestic cat and cheetah FHV-1 isolates. Collectively, these data suggest that the MLV is capable of causing clinical disease and viral shedding in some cheetahs and represents evidence of interspecies transmission of virus between domestic and wild cats.
format	article
author	Morgan E. Marino Melanie A. Mironovich Nikole E. Ineck Scott B. Citino Jessica A. Emerson David J. Maggs Lyndon M. Coghill Edward J. Dubovi Rachel C. Turner Renee T. Carter Andrew C. Lewin
author_facet	Morgan E. Marino Melanie A. Mironovich Nikole E. Ineck Scott B. Citino Jessica A. Emerson David J. Maggs Lyndon M. Coghill Edward J. Dubovi Rachel C. Turner Renee T. Carter Andrew C. Lewin
author_sort	Morgan E. Marino
title	Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>)
title_short	Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>)
title_full	Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>)
title_fullStr	Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>)
title_full_unstemmed	Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>)
title_sort	full viral genome sequencing and phylogenomic analysis of feline herpesvirus type 1 (fhv-1) in cheetahs (<i>acinonyx jubatus</i>)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0477551e34924dbcbf0ea52b409f2c3d
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Full Viral Genome Sequencing and Phylogenomic Analysis of Feline Herpesvirus Type 1 (FHV-1) in Cheetahs (<i>Acinonyx jubatus</i>)

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