Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells

Abstract The aim of the current study was to develop an iron oxide nanoparticle (ION) labelling and magnetic resonance imaging (MRI)-based protocol to allow visualization of the differentiation process of mesenchymal stem cells (MSCs) into neural-like cells (NCs) in vitro. Ferucarbotran, a clinicall...

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Autores principales:	Chen-Wen Lu, Jong-Kai Hsiao, Hon-Man Liu, Chung-Hsin Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0477f8c61300486fbd1d954abfb49f7d
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spelling	oai:doaj.org-article:0477f8c61300486fbd1d954abfb49f7d2021-12-02T15:05:17ZCharacterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells10.1038/s41598-017-03863-x2045-2322https://doaj.org/article/0477f8c61300486fbd1d954abfb49f7d2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03863-xhttps://doaj.org/toc/2045-2322Abstract The aim of the current study was to develop an iron oxide nanoparticle (ION) labelling and magnetic resonance imaging (MRI)-based protocol to allow visualization of the differentiation process of mesenchymal stem cells (MSCs) into neural-like cells (NCs) in vitro. Ferucarbotran, a clinically available ION, which can be visualized under MRI, is used for tracking cells implanted in vivo. The NCs were verified morphologically and histologically by light microscopy, and their functions were verified by measuring their action potentials. Conformational conversion of axon-like structures was observed under light microscopy. These NCs exhibited frequent, active action potentials compared with cells that did not undergo neural differentiation. The labelling of ION had no influence on the morphological and functional differentiation capacity of the MSCs. We conclude that the MSCs that were differentiated into NCs exhibited in vitro activity potential firing and may be used to replace damaged neurons.Chen-Wen LuJong-Kai HsiaoHon-Man LiuChung-Hsin WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chen-Wen Lu Jong-Kai Hsiao Hon-Man Liu Chung-Hsin Wu Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells
description	Abstract The aim of the current study was to develop an iron oxide nanoparticle (ION) labelling and magnetic resonance imaging (MRI)-based protocol to allow visualization of the differentiation process of mesenchymal stem cells (MSCs) into neural-like cells (NCs) in vitro. Ferucarbotran, a clinically available ION, which can be visualized under MRI, is used for tracking cells implanted in vivo. The NCs were verified morphologically and histologically by light microscopy, and their functions were verified by measuring their action potentials. Conformational conversion of axon-like structures was observed under light microscopy. These NCs exhibited frequent, active action potentials compared with cells that did not undergo neural differentiation. The labelling of ION had no influence on the morphological and functional differentiation capacity of the MSCs. We conclude that the MSCs that were differentiated into NCs exhibited in vitro activity potential firing and may be used to replace damaged neurons.
format	article
author	Chen-Wen Lu Jong-Kai Hsiao Hon-Man Liu Chung-Hsin Wu
author_facet	Chen-Wen Lu Jong-Kai Hsiao Hon-Man Liu Chung-Hsin Wu
author_sort	Chen-Wen Lu
title	Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells
title_short	Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells
title_full	Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells
title_fullStr	Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells
title_full_unstemmed	Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells
title_sort	characterization of an iron oxide nanoparticle labelling and mri-based protocol for inducing human mesenchymal stem cells into neural-like cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0477f8c61300486fbd1d954abfb49f7d
work_keys_str_mv	AT chenwenlu characterizationofanironoxidenanoparticlelabellingandmribasedprotocolforinducinghumanmesenchymalstemcellsintoneurallikecells AT jongkaihsiao characterizationofanironoxidenanoparticlelabellingandmribasedprotocolforinducinghumanmesenchymalstemcellsintoneurallikecells AT honmanliu characterizationofanironoxidenanoparticlelabellingandmribasedprotocolforinducinghumanmesenchymalstemcellsintoneurallikecells AT chunghsinwu characterizationofanironoxidenanoparticlelabellingandmribasedprotocolforinducinghumanmesenchymalstemcellsintoneurallikecells
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Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells

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