Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation

Abstract The diagnosis of kidney allograft rejection is based on late histological and clinical markers. Early, specific and minimally-invasive biomarkers may improve rejection diagnosis. Endothelial cells (EC) are one of the earliest targets in kidney transplant rejection. We investigated whether c...

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Autores principales:	H. Tejeda-Mora, J. G. H. P. Verhoeven, W. Verschoor, K. Boer, D. A. Hesselink, M. W. F. van den Hoogen, L. J. W. van der Laan, C. C. Baan, R. C. Minnee, M. J. Hoogduijn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/04791b937768459698233c55be9dff03
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spelling	oai:doaj.org-article:04791b937768459698233c55be9dff032021-12-02T13:41:22ZCirculating endothelial cells transiently increase in peripheral blood after kidney transplantation10.1038/s41598-021-88411-42045-2322https://doaj.org/article/04791b937768459698233c55be9dff032021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88411-4https://doaj.org/toc/2045-2322Abstract The diagnosis of kidney allograft rejection is based on late histological and clinical markers. Early, specific and minimally-invasive biomarkers may improve rejection diagnosis. Endothelial cells (EC) are one of the earliest targets in kidney transplant rejection. We investigated whether circulating EC (cEC) could serve as an earlier and less invasive biomarker for allograft rejection. Blood was collected from a cohort of 51 kidney transplant recipients before and at multiple timepoints after transplantation, including during a for cause biopsy. The number and phenotype of EC was assessed by flow-cytometric analysis. Unbiased selection of EC was done using principal component (PCA) analysis. Paired analysis revealed a transient cEC increase of 2.1-fold on the third day post-transplant, recovering to preoperative levels at seventh day post-transplant and onwards. Analysis of HLA subtype demonstrated that cEC mainly originate from the recipient. cEC levels were not associated with allograft rejection, allograft function or other allograft pathologies. However, cEC in patients with allograft rejection and increased levels of cEC showed elevated levels of KIM-1 (kidney injury marker-1). These findings indicate that cEC numbers and phenotype are affected after kidney transplantation but may not improve rejection diagnosis.H. Tejeda-MoraJ. G. H. P. VerhoevenW. VerschoorK. BoerD. A. HesselinkM. W. F. van den HoogenL. J. W. van der LaanC. C. BaanR. C. MinneeM. J. HoogduijnNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q H. Tejeda-Mora J. G. H. P. Verhoeven W. Verschoor K. Boer D. A. Hesselink M. W. F. van den Hoogen L. J. W. van der Laan C. C. Baan R. C. Minnee M. J. Hoogduijn Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation
description	Abstract The diagnosis of kidney allograft rejection is based on late histological and clinical markers. Early, specific and minimally-invasive biomarkers may improve rejection diagnosis. Endothelial cells (EC) are one of the earliest targets in kidney transplant rejection. We investigated whether circulating EC (cEC) could serve as an earlier and less invasive biomarker for allograft rejection. Blood was collected from a cohort of 51 kidney transplant recipients before and at multiple timepoints after transplantation, including during a for cause biopsy. The number and phenotype of EC was assessed by flow-cytometric analysis. Unbiased selection of EC was done using principal component (PCA) analysis. Paired analysis revealed a transient cEC increase of 2.1-fold on the third day post-transplant, recovering to preoperative levels at seventh day post-transplant and onwards. Analysis of HLA subtype demonstrated that cEC mainly originate from the recipient. cEC levels were not associated with allograft rejection, allograft function or other allograft pathologies. However, cEC in patients with allograft rejection and increased levels of cEC showed elevated levels of KIM-1 (kidney injury marker-1). These findings indicate that cEC numbers and phenotype are affected after kidney transplantation but may not improve rejection diagnosis.
format	article
author	H. Tejeda-Mora J. G. H. P. Verhoeven W. Verschoor K. Boer D. A. Hesselink M. W. F. van den Hoogen L. J. W. van der Laan C. C. Baan R. C. Minnee M. J. Hoogduijn
author_facet	H. Tejeda-Mora J. G. H. P. Verhoeven W. Verschoor K. Boer D. A. Hesselink M. W. F. van den Hoogen L. J. W. van der Laan C. C. Baan R. C. Minnee M. J. Hoogduijn
author_sort	H. Tejeda-Mora
title	Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation
title_short	Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation
title_full	Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation
title_fullStr	Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation
title_full_unstemmed	Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation
title_sort	circulating endothelial cells transiently increase in peripheral blood after kidney transplantation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/04791b937768459698233c55be9dff03
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_version_	1718392537477021696

Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation

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