Activation of Adenosine Monophosphate-Activated Protein Kinase Drives the Aerobic Glycolysis in Hippocampus for Delaying Cognitive Decline Following Electroacupuncture Treatment in APP/PS1 Mice
Aerobic glycolysis (AG), an important pathway of glucose metabolism, is dramatically declined in Alzheimer’s disease (AD). AMP-activated protein kinase (AMPK) is a key regulator to maintain the stability of energy metabolism by promoting the process of AG and regulating glucose metabolism. Interesti...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:047b4c9cce5c42c5bfc2ac335576a0982021-11-16T07:37:24ZActivation of Adenosine Monophosphate-Activated Protein Kinase Drives the Aerobic Glycolysis in Hippocampus for Delaying Cognitive Decline Following Electroacupuncture Treatment in APP/PS1 Mice1662-510210.3389/fncel.2021.774569https://doaj.org/article/047b4c9cce5c42c5bfc2ac335576a0982021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fncel.2021.774569/fullhttps://doaj.org/toc/1662-5102Aerobic glycolysis (AG), an important pathway of glucose metabolism, is dramatically declined in Alzheimer’s disease (AD). AMP-activated protein kinase (AMPK) is a key regulator to maintain the stability of energy metabolism by promoting the process of AG and regulating glucose metabolism. Interestingly, it has been previously reported that electroacupuncture (EA) treatment can improve cognitive function in AD through the enhancement of glucose metabolism. In this study, we generated AMPK-knockdown mice to confirm the EA effect on AMPK activation and further clarify the mechanism of EA in regulating energy metabolism and improving cognitive function in APP/PS1 mice. The behavioral results showed that EA treatment can improve the learning and memory abilities in APP/PS1 mice. At the same time, the glucose metabolism in the hippocampus was increased detected by MRI-chemical exchange saturation transfer (MRI-CEST). The expression of proteins associated with AG in the hippocampus was increased simultaneously, including hexokinase II (HK2), 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), and pyruvate kinase M2 (PKM2). Moreover, the knockdown of AMPK attenuated AG activated by EA treatment. In conclusion, this study proves that EA can activate AMPK to enhance the process of AG in the early stage of AD.Jianhong LiBingxue ZhangWeiwei JiaMinguang YangYuhao ZhangJiayong ZhangLe LiTingting JinZhifu WangJing TaoLidian ChenShengxiang LiangWeilin LiuFrontiers Media S.A.articleelectroacupunctureAlzheimer’s diseaseaerobic glycolysislearning and memoryadenosine monophosphate-activated protein kinase (AMPK)Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Cellular Neuroscience, Vol 15 (2021) |
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| collection |
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| topic |
electroacupuncture Alzheimer’s disease aerobic glycolysis learning and memory adenosine monophosphate-activated protein kinase (AMPK) Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 |
| spellingShingle |
electroacupuncture Alzheimer’s disease aerobic glycolysis learning and memory adenosine monophosphate-activated protein kinase (AMPK) Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Jianhong Li Bingxue Zhang Weiwei Jia Minguang Yang Yuhao Zhang Jiayong Zhang Le Li Tingting Jin Zhifu Wang Jing Tao Lidian Chen Shengxiang Liang Weilin Liu Activation of Adenosine Monophosphate-Activated Protein Kinase Drives the Aerobic Glycolysis in Hippocampus for Delaying Cognitive Decline Following Electroacupuncture Treatment in APP/PS1 Mice |
| description |
Aerobic glycolysis (AG), an important pathway of glucose metabolism, is dramatically declined in Alzheimer’s disease (AD). AMP-activated protein kinase (AMPK) is a key regulator to maintain the stability of energy metabolism by promoting the process of AG and regulating glucose metabolism. Interestingly, it has been previously reported that electroacupuncture (EA) treatment can improve cognitive function in AD through the enhancement of glucose metabolism. In this study, we generated AMPK-knockdown mice to confirm the EA effect on AMPK activation and further clarify the mechanism of EA in regulating energy metabolism and improving cognitive function in APP/PS1 mice. The behavioral results showed that EA treatment can improve the learning and memory abilities in APP/PS1 mice. At the same time, the glucose metabolism in the hippocampus was increased detected by MRI-chemical exchange saturation transfer (MRI-CEST). The expression of proteins associated with AG in the hippocampus was increased simultaneously, including hexokinase II (HK2), 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), and pyruvate kinase M2 (PKM2). Moreover, the knockdown of AMPK attenuated AG activated by EA treatment. In conclusion, this study proves that EA can activate AMPK to enhance the process of AG in the early stage of AD. |
| format |
article |
| author |
Jianhong Li Bingxue Zhang Weiwei Jia Minguang Yang Yuhao Zhang Jiayong Zhang Le Li Tingting Jin Zhifu Wang Jing Tao Lidian Chen Shengxiang Liang Weilin Liu |
| author_facet |
Jianhong Li Bingxue Zhang Weiwei Jia Minguang Yang Yuhao Zhang Jiayong Zhang Le Li Tingting Jin Zhifu Wang Jing Tao Lidian Chen Shengxiang Liang Weilin Liu |
| author_sort |
Jianhong Li |
| title |
Activation of Adenosine Monophosphate-Activated Protein Kinase Drives the Aerobic Glycolysis in Hippocampus for Delaying Cognitive Decline Following Electroacupuncture Treatment in APP/PS1 Mice |
| title_short |
Activation of Adenosine Monophosphate-Activated Protein Kinase Drives the Aerobic Glycolysis in Hippocampus for Delaying Cognitive Decline Following Electroacupuncture Treatment in APP/PS1 Mice |
| title_full |
Activation of Adenosine Monophosphate-Activated Protein Kinase Drives the Aerobic Glycolysis in Hippocampus for Delaying Cognitive Decline Following Electroacupuncture Treatment in APP/PS1 Mice |
| title_fullStr |
Activation of Adenosine Monophosphate-Activated Protein Kinase Drives the Aerobic Glycolysis in Hippocampus for Delaying Cognitive Decline Following Electroacupuncture Treatment in APP/PS1 Mice |
| title_full_unstemmed |
Activation of Adenosine Monophosphate-Activated Protein Kinase Drives the Aerobic Glycolysis in Hippocampus for Delaying Cognitive Decline Following Electroacupuncture Treatment in APP/PS1 Mice |
| title_sort |
activation of adenosine monophosphate-activated protein kinase drives the aerobic glycolysis in hippocampus for delaying cognitive decline following electroacupuncture treatment in app/ps1 mice |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/047b4c9cce5c42c5bfc2ac335576a098 |
| work_keys_str_mv |
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