Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon

This research reveals the improved performance of bioelectrodes made with amination-modified glucose oxidase (GOx-NH<sub>2</sub>) and carboxyl-functionalized mesoporous carbon (OMC-COOH). Results showed that when applied with 10 mM EDC amination, the functional groups of NH<sub>2&l...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chuhan Lv, Xuewei Yang, Zongkang Wang, Ming Ying, Qingguo Han, Shuangfei Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	carboxylation chemical amination glucose oxidase Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/047dfcac706f4403867a7449d1b45e49
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:047dfcac706f4403867a7449d1b45e49
record_format	dspace
spelling	oai:doaj.org-article:047dfcac706f4403867a7449d1b45e492021-11-25T18:32:20ZEnhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon10.3390/nano111130862079-4991https://doaj.org/article/047dfcac706f4403867a7449d1b45e492021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/3086https://doaj.org/toc/2079-4991This research reveals the improved performance of bioelectrodes made with amination-modified glucose oxidase (GOx-NH<sub>2</sub>) and carboxyl-functionalized mesoporous carbon (OMC-COOH). Results showed that when applied with 10 mM EDC amination, the functional groups of NH<sub>2</sub> were successfully added to GOx, according to the analysis of <sup>1</sup>H-NMR, elemental composition, and FTIR spectra. Moreover, after the aminated modification, increased enzyme immobilization (124.01 ± 1.49 mg GOx-NH<sub>2</sub>/g OMC-COOH; 2.77-fold increase) and enzyme activity (1.17-fold increase) were achieved, compared with those of non-modified GOx. Electrochemical analysis showed that aminated modification enhanced the peak current intensity of Nafion/GOx-NH<sub>2</sub>/OMC-COOH (1.32-fold increase), with increases in the charge transfer coefficient <i>α</i> (0.54), the apparent electron transfer rate constant <i>k<sub>s</sub></i> (2.54 s<sup>−1</sup>), and the surface coverage <i>Γ</i> (2.91 × 10<sup>−9</sup> mol·cm<sup>−2</sup>). Results showed that GOx-NH<sub>2</sub>/OMC-COOH exhibited impressive electro-activity and a favorable anodic reaction.Chuhan LvXuewei YangZongkang WangMing YingQingguo HanShuangfei LiMDPI AGarticlecarboxylationchemical aminationglucose oxidaseChemistryQD1-999ENNanomaterials, Vol 11, Iss 3086, p 3086 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	carboxylation chemical amination glucose oxidase Chemistry QD1-999
spellingShingle	carboxylation chemical amination glucose oxidase Chemistry QD1-999 Chuhan Lv Xuewei Yang Zongkang Wang Ming Ying Qingguo Han Shuangfei Li Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon
description	This research reveals the improved performance of bioelectrodes made with amination-modified glucose oxidase (GOx-NH<sub>2</sub>) and carboxyl-functionalized mesoporous carbon (OMC-COOH). Results showed that when applied with 10 mM EDC amination, the functional groups of NH<sub>2</sub> were successfully added to GOx, according to the analysis of <sup>1</sup>H-NMR, elemental composition, and FTIR spectra. Moreover, after the aminated modification, increased enzyme immobilization (124.01 ± 1.49 mg GOx-NH<sub>2</sub>/g OMC-COOH; 2.77-fold increase) and enzyme activity (1.17-fold increase) were achieved, compared with those of non-modified GOx. Electrochemical analysis showed that aminated modification enhanced the peak current intensity of Nafion/GOx-NH<sub>2</sub>/OMC-COOH (1.32-fold increase), with increases in the charge transfer coefficient <i>α</i> (0.54), the apparent electron transfer rate constant <i>k<sub>s</sub></i> (2.54 s<sup>−1</sup>), and the surface coverage <i>Γ</i> (2.91 × 10<sup>−9</sup> mol·cm<sup>−2</sup>). Results showed that GOx-NH<sub>2</sub>/OMC-COOH exhibited impressive electro-activity and a favorable anodic reaction.
format	article
author	Chuhan Lv Xuewei Yang Zongkang Wang Ming Ying Qingguo Han Shuangfei Li
author_facet	Chuhan Lv Xuewei Yang Zongkang Wang Ming Ying Qingguo Han Shuangfei Li
author_sort	Chuhan Lv
title	Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon
title_short	Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon
title_full	Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon
title_fullStr	Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon
title_full_unstemmed	Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon
title_sort	enhanced performance of bioelectrodes made with amination-modified glucose oxidase immobilized on carboxyl-functionalized ordered mesoporous carbon
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/047dfcac706f4403867a7449d1b45e49
work_keys_str_mv	AT chuhanlv enhancedperformanceofbioelectrodesmadewithaminationmodifiedglucoseoxidaseimmobilizedoncarboxylfunctionalizedorderedmesoporouscarbon AT xueweiyang enhancedperformanceofbioelectrodesmadewithaminationmodifiedglucoseoxidaseimmobilizedoncarboxylfunctionalizedorderedmesoporouscarbon AT zongkangwang enhancedperformanceofbioelectrodesmadewithaminationmodifiedglucoseoxidaseimmobilizedoncarboxylfunctionalizedorderedmesoporouscarbon AT mingying enhancedperformanceofbioelectrodesmadewithaminationmodifiedglucoseoxidaseimmobilizedoncarboxylfunctionalizedorderedmesoporouscarbon AT qingguohan enhancedperformanceofbioelectrodesmadewithaminationmodifiedglucoseoxidaseimmobilizedoncarboxylfunctionalizedorderedmesoporouscarbon AT shuangfeili enhancedperformanceofbioelectrodesmadewithaminationmodifiedglucoseoxidaseimmobilizedoncarboxylfunctionalizedorderedmesoporouscarbon
_version_	1718410995903234048

Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon

Ejemplares similares