Screening Plasma Exosomal RNAs as Diagnostic Markers for Cervical Cancer: An Analysis of Patients Who Underwent Primary Chemoradiotherapy

This preliminary study aimed to screen non-coding RNAs (ncRNAs) from plasma exosomes as a new method for cervical cancer diagnosis. Differentially expressed RNAs were initially selected from among a group of 12 healthy individuals (normal group) and a pretreatment group of 30 patients with cervical...

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Autores principales:	Oyeon Cho, Do-Wan Kim, Jae-Youn Cheong
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cervical cancer plasma exosomes non-coding RNA mRNA cancer screen radiation therapy Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/04999f49efeb4bb7b8c875ff617e31b5
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spelling	oai:doaj.org-article:04999f49efeb4bb7b8c875ff617e31b52021-11-25T16:53:51ZScreening Plasma Exosomal RNAs as Diagnostic Markers for Cervical Cancer: An Analysis of Patients Who Underwent Primary Chemoradiotherapy10.3390/biom111116912218-273Xhttps://doaj.org/article/04999f49efeb4bb7b8c875ff617e31b52021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1691https://doaj.org/toc/2218-273XThis preliminary study aimed to screen non-coding RNAs (ncRNAs) from plasma exosomes as a new method for cervical cancer diagnosis. Differentially expressed RNAs were initially selected from among a group of 12 healthy individuals (normal group) and a pretreatment group of 30 patients with cervical cancer (cancer group). Then, we analyzed the association between an ncRNA-mRNA network and cancer using ingenuity pathway analysis after secondary selection according to the number and correlation of mRNAs (or ncRNAs) relative to changes in the expression of primarily selected ncRNAs (or mRNAs) before and after chemoradiotherapy. The number of RNAs selected from the initial RNAs was one from 13 miRNAs, four from 42 piRNAs, four from 28 lncRNAs, nine from 18 snoRNAs, 10 from 76 snRNAs, nine from 474 tRNAs, nine from 64 yRNAs, and five from 67 mRNAs. The combination of miRNA (miR-142-3p), mRNAs (<i>CXCL5, KIF2A, RGS18, APL6IP5</i>, and <i>DAPP1</i>), and snoRNAs (SNORD17, SCARNA12, SNORA6, SNORA12, SCRNA1, SNORD97, SNORD62, and SNORD38A) clearly distinguished the normal samples from the cancer group samples. We present a method for efficiently screening eight classes of RNAs isolated from exosomes for cervical cancer diagnosis using mRNAs (or ncRNAs) altered by chemoradiotherapy.Oyeon ChoDo-Wan KimJae-Youn CheongMDPI AGarticlecervical cancerplasma exosomesnon-coding RNAmRNAcancer screenradiation therapyMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1691, p 1691 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cervical cancer plasma exosomes non-coding RNA mRNA cancer screen radiation therapy Microbiology QR1-502
spellingShingle	cervical cancer plasma exosomes non-coding RNA mRNA cancer screen radiation therapy Microbiology QR1-502 Oyeon Cho Do-Wan Kim Jae-Youn Cheong Screening Plasma Exosomal RNAs as Diagnostic Markers for Cervical Cancer: An Analysis of Patients Who Underwent Primary Chemoradiotherapy
description	This preliminary study aimed to screen non-coding RNAs (ncRNAs) from plasma exosomes as a new method for cervical cancer diagnosis. Differentially expressed RNAs were initially selected from among a group of 12 healthy individuals (normal group) and a pretreatment group of 30 patients with cervical cancer (cancer group). Then, we analyzed the association between an ncRNA-mRNA network and cancer using ingenuity pathway analysis after secondary selection according to the number and correlation of mRNAs (or ncRNAs) relative to changes in the expression of primarily selected ncRNAs (or mRNAs) before and after chemoradiotherapy. The number of RNAs selected from the initial RNAs was one from 13 miRNAs, four from 42 piRNAs, four from 28 lncRNAs, nine from 18 snoRNAs, 10 from 76 snRNAs, nine from 474 tRNAs, nine from 64 yRNAs, and five from 67 mRNAs. The combination of miRNA (miR-142-3p), mRNAs (<i>CXCL5, KIF2A, RGS18, APL6IP5</i>, and <i>DAPP1</i>), and snoRNAs (SNORD17, SCARNA12, SNORA6, SNORA12, SCRNA1, SNORD97, SNORD62, and SNORD38A) clearly distinguished the normal samples from the cancer group samples. We present a method for efficiently screening eight classes of RNAs isolated from exosomes for cervical cancer diagnosis using mRNAs (or ncRNAs) altered by chemoradiotherapy.
format	article
author	Oyeon Cho Do-Wan Kim Jae-Youn Cheong
author_facet	Oyeon Cho Do-Wan Kim Jae-Youn Cheong
author_sort	Oyeon Cho
title	Screening Plasma Exosomal RNAs as Diagnostic Markers for Cervical Cancer: An Analysis of Patients Who Underwent Primary Chemoradiotherapy
title_short	Screening Plasma Exosomal RNAs as Diagnostic Markers for Cervical Cancer: An Analysis of Patients Who Underwent Primary Chemoradiotherapy
title_full	Screening Plasma Exosomal RNAs as Diagnostic Markers for Cervical Cancer: An Analysis of Patients Who Underwent Primary Chemoradiotherapy
title_fullStr	Screening Plasma Exosomal RNAs as Diagnostic Markers for Cervical Cancer: An Analysis of Patients Who Underwent Primary Chemoradiotherapy
title_full_unstemmed	Screening Plasma Exosomal RNAs as Diagnostic Markers for Cervical Cancer: An Analysis of Patients Who Underwent Primary Chemoradiotherapy
title_sort	screening plasma exosomal rnas as diagnostic markers for cervical cancer: an analysis of patients who underwent primary chemoradiotherapy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/04999f49efeb4bb7b8c875ff617e31b5
work_keys_str_mv	AT oyeoncho screeningplasmaexosomalrnasasdiagnosticmarkersforcervicalcancerananalysisofpatientswhounderwentprimarychemoradiotherapy AT dowankim screeningplasmaexosomalrnasasdiagnosticmarkersforcervicalcancerananalysisofpatientswhounderwentprimarychemoradiotherapy AT jaeyouncheong screeningplasmaexosomalrnasasdiagnosticmarkersforcervicalcancerananalysisofpatientswhounderwentprimarychemoradiotherapy
_version_	1718412816790061056

Screening Plasma Exosomal RNAs as Diagnostic Markers for Cervical Cancer: An Analysis of Patients Who Underwent Primary Chemoradiotherapy

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