Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions

Metal ions in high concentrations can pollute the marine environment. Human activities and industrial pollution are the causes of Cu<sup>2+</sup> contamination. Here, we report our discovery of an enzyme method-based microfluidic that can be used to rapidly detect Cu<sup>2+</sup...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Binfeng Yin, Xinhua Wan, Changcheng Qian, A. S. M. Muhtasim Fuad Sohan, Teng Zhou, Wenkai Yue
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biological logic gate Cu<sup>2+</sup> detection microfluidic chip Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/049ba2f0a7314564bf66d5cbcf11e943
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:049ba2f0a7314564bf66d5cbcf11e943
record_format	dspace
spelling	oai:doaj.org-article:049ba2f0a7314564bf66d5cbcf11e9432021-11-25T18:23:32ZEnzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions10.3390/mi121113802072-666Xhttps://doaj.org/article/049ba2f0a7314564bf66d5cbcf11e9432021-11-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1380https://doaj.org/toc/2072-666XMetal ions in high concentrations can pollute the marine environment. Human activities and industrial pollution are the causes of Cu<sup>2+</sup> contamination. Here, we report our discovery of an enzyme method-based microfluidic that can be used to rapidly detect Cu<sup>2+</sup> in seawater. In this method, Cu<sup>2+</sup> is reduced to Cu<sup>+</sup> to inhibit horseradish peroxidase (HRP) activity, which then results in the color distortion of the reaction solution. The chip provides both naked eye and spectrophotometer modalities. Cu<sup>2+</sup> concentrations have an ideal linear relationship, with absorbance values ranging from 3.91 nM to 256 μM. The proposed enzyme method-based microfluidic chip detects Cu<sup>2+</sup> with a limit of detection (LOD) of 0.87 nM. Other common metal ions do not affect the operation of the chip. The successful detection of Cu<sup>2+</sup> was achieved using three real seawater samples, verifying the ability of the chip in practical applications. Furthermore, the chip realizes the functions of two AND gates in series and has potential practical implementations in biochemical detection and biological computing.Binfeng YinXinhua WanChangcheng QianA. S. M. Muhtasim Fuad SohanTeng ZhouWenkai YueMDPI AGarticlebiological logic gateCu<sup>2+</sup> detectionmicrofluidic chipMechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1380, p 1380 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biological logic gate Cu<sup>2+</sup> detection microfluidic chip Mechanical engineering and machinery TJ1-1570
spellingShingle	biological logic gate Cu<sup>2+</sup> detection microfluidic chip Mechanical engineering and machinery TJ1-1570 Binfeng Yin Xinhua Wan Changcheng Qian A. S. M. Muhtasim Fuad Sohan Teng Zhou Wenkai Yue Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions
description	Metal ions in high concentrations can pollute the marine environment. Human activities and industrial pollution are the causes of Cu<sup>2+</sup> contamination. Here, we report our discovery of an enzyme method-based microfluidic that can be used to rapidly detect Cu<sup>2+</sup> in seawater. In this method, Cu<sup>2+</sup> is reduced to Cu<sup>+</sup> to inhibit horseradish peroxidase (HRP) activity, which then results in the color distortion of the reaction solution. The chip provides both naked eye and spectrophotometer modalities. Cu<sup>2+</sup> concentrations have an ideal linear relationship, with absorbance values ranging from 3.91 nM to 256 μM. The proposed enzyme method-based microfluidic chip detects Cu<sup>2+</sup> with a limit of detection (LOD) of 0.87 nM. Other common metal ions do not affect the operation of the chip. The successful detection of Cu<sup>2+</sup> was achieved using three real seawater samples, verifying the ability of the chip in practical applications. Furthermore, the chip realizes the functions of two AND gates in series and has potential practical implementations in biochemical detection and biological computing.
format	article
author	Binfeng Yin Xinhua Wan Changcheng Qian A. S. M. Muhtasim Fuad Sohan Teng Zhou Wenkai Yue
author_facet	Binfeng Yin Xinhua Wan Changcheng Qian A. S. M. Muhtasim Fuad Sohan Teng Zhou Wenkai Yue
author_sort	Binfeng Yin
title	Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions
title_short	Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions
title_full	Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions
title_fullStr	Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions
title_full_unstemmed	Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions
title_sort	enzyme method-based microfluidic chip for the rapid detection of copper ions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/049ba2f0a7314564bf66d5cbcf11e943
work_keys_str_mv	AT binfengyin enzymemethodbasedmicrofluidicchipfortherapiddetectionofcopperions AT xinhuawan enzymemethodbasedmicrofluidicchipfortherapiddetectionofcopperions AT changchengqian enzymemethodbasedmicrofluidicchipfortherapiddetectionofcopperions AT asmmuhtasimfuadsohan enzymemethodbasedmicrofluidicchipfortherapiddetectionofcopperions AT tengzhou enzymemethodbasedmicrofluidicchipfortherapiddetectionofcopperions AT wenkaiyue enzymemethodbasedmicrofluidicchipfortherapiddetectionofcopperions
_version_	1718411175793786880

Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions

Ejemplares similares