Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize

Abstract The hemibiotrophic fungus Colletotrichum graminicola may cause severe damage to maize, affecting normal development of the plant and decreasing grain yield. In this context, understanding plant defense pathways at the inoculation site and systemically in uninoculated tissues can help in the...

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Autores principales:	Vívian de Jesus Miranda, William Farias Porto, Gabriel da Rocha Fernandes, Robert Pogue, Diego Oliveira Nolasco, Ana Claudia Guerra Araujo, Luciano Viana Cota, Camila Guimarães de Freitas, Simoni Campos Dias, Octavio Luiz Franco
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/049c66ade81d46c3bf2d17cd6302131c
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spelling	oai:doaj.org-article:049c66ade81d46c3bf2d17cd6302131c2021-12-02T15:04:56ZComparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize10.1038/s41598-017-02298-82045-2322https://doaj.org/article/049c66ade81d46c3bf2d17cd6302131c2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02298-8https://doaj.org/toc/2045-2322Abstract The hemibiotrophic fungus Colletotrichum graminicola may cause severe damage to maize, affecting normal development of the plant and decreasing grain yield. In this context, understanding plant defense pathways at the inoculation site and systemically in uninoculated tissues can help in the development of genetic engineering of resistance against this pathogen. Previous work has discussed the molecular basis of maize - C. graminicola interaction. However, many genes involved in defense have not yet been exploited for lack of annotation in public databases. Here, changes in global gene expression were studied in root, male and female inflorescences of maize under local and systemic fungal infection treatments, respectively. RNA-Seq with qPCR was used to indicate genes involved in plant defense. We found that systemic acquired resistance induction in female inflorescences mainly involves accumulation of salicylic acid (SA)-inducible defense genes (ZmNAC, ZmHSF, ZmWRKY, ZmbZIP and PR1) and potential genes involved in chromatin modification. Furthermore, transcripts involved in jasmonic acid (JA) and ethylene (ET) signaling pathways were also accumulated and may participate in plant immunity. Moreover, several genes were functionally re-annotated based on domain signature, indicating novel candidates to be tested in strategies involving gene knockout and overexpression in plants.Vívian de Jesus MirandaWilliam Farias PortoGabriel da Rocha FernandesRobert PogueDiego Oliveira NolascoAna Claudia Guerra AraujoLuciano Viana CotaCamila Guimarães de FreitasSimoni Campos DiasOctavio Luiz FrancoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vívian de Jesus Miranda William Farias Porto Gabriel da Rocha Fernandes Robert Pogue Diego Oliveira Nolasco Ana Claudia Guerra Araujo Luciano Viana Cota Camila Guimarães de Freitas Simoni Campos Dias Octavio Luiz Franco Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize
description	Abstract The hemibiotrophic fungus Colletotrichum graminicola may cause severe damage to maize, affecting normal development of the plant and decreasing grain yield. In this context, understanding plant defense pathways at the inoculation site and systemically in uninoculated tissues can help in the development of genetic engineering of resistance against this pathogen. Previous work has discussed the molecular basis of maize - C. graminicola interaction. However, many genes involved in defense have not yet been exploited for lack of annotation in public databases. Here, changes in global gene expression were studied in root, male and female inflorescences of maize under local and systemic fungal infection treatments, respectively. RNA-Seq with qPCR was used to indicate genes involved in plant defense. We found that systemic acquired resistance induction in female inflorescences mainly involves accumulation of salicylic acid (SA)-inducible defense genes (ZmNAC, ZmHSF, ZmWRKY, ZmbZIP and PR1) and potential genes involved in chromatin modification. Furthermore, transcripts involved in jasmonic acid (JA) and ethylene (ET) signaling pathways were also accumulated and may participate in plant immunity. Moreover, several genes were functionally re-annotated based on domain signature, indicating novel candidates to be tested in strategies involving gene knockout and overexpression in plants.
format	article
author	Vívian de Jesus Miranda William Farias Porto Gabriel da Rocha Fernandes Robert Pogue Diego Oliveira Nolasco Ana Claudia Guerra Araujo Luciano Viana Cota Camila Guimarães de Freitas Simoni Campos Dias Octavio Luiz Franco
author_facet	Vívian de Jesus Miranda William Farias Porto Gabriel da Rocha Fernandes Robert Pogue Diego Oliveira Nolasco Ana Claudia Guerra Araujo Luciano Viana Cota Camila Guimarães de Freitas Simoni Campos Dias Octavio Luiz Franco
author_sort	Vívian de Jesus Miranda
title	Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize
title_short	Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize
title_full	Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize
title_fullStr	Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize
title_full_unstemmed	Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize
title_sort	comparative transcriptomic analysis indicates genes associated with local and systemic resistance to colletotrichum graminicola in maize
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/049c66ade81d46c3bf2d17cd6302131c
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Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize

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