Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota

In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis. RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cyt...

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Autores principales:	Gichang Kim, Mi Jang, Inguk Hwang, Jeongyong Cho, Saehun Kim
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Colitis Inflammatory cytokines Microbiota Radish sprout (RS) Dextran sulfate sodium (DSS) Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/049e5d36b1e74287a4415976d1b065b2
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spelling	oai:doaj.org-article:049e5d36b1e74287a4415976d1b065b22021-11-14T04:30:23ZRadish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota0753-332210.1016/j.biopha.2021.112365https://doaj.org/article/049e5d36b1e74287a4415976d1b065b22021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221011495https://doaj.org/toc/0753-3322In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis. RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages. In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis. The diversity of the fecal microbiota was significantly increased in the group treated with RSE. In addition, RSE administration decreased the relative abundance of the phylum Proteobacteria, which includes many pathogens, and increased the abundance of the genus Akkermansia. Beta diversity analyses showed that RSE administration restored the gut microbiota composition close to that of healthy mice. For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects. Notably, 1,2-O-disinapoyl glucoside substantially decreased nitric oxide generation in LPS-stimulated macrophages.Gichang KimMi JangInguk HwangJeongyong ChoSaehun KimElsevierarticleColitisInflammatory cytokinesMicrobiotaRadish sprout (RS)Dextran sulfate sodium (DSS)Therapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112365- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Colitis Inflammatory cytokines Microbiota Radish sprout (RS) Dextran sulfate sodium (DSS) Therapeutics. Pharmacology RM1-950
spellingShingle	Colitis Inflammatory cytokines Microbiota Radish sprout (RS) Dextran sulfate sodium (DSS) Therapeutics. Pharmacology RM1-950 Gichang Kim Mi Jang Inguk Hwang Jeongyong Cho Saehun Kim Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota
description	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis. RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages. In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis. The diversity of the fecal microbiota was significantly increased in the group treated with RSE. In addition, RSE administration decreased the relative abundance of the phylum Proteobacteria, which includes many pathogens, and increased the abundance of the genus Akkermansia. Beta diversity analyses showed that RSE administration restored the gut microbiota composition close to that of healthy mice. For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects. Notably, 1,2-O-disinapoyl glucoside substantially decreased nitric oxide generation in LPS-stimulated macrophages.
format	article
author	Gichang Kim Mi Jang Inguk Hwang Jeongyong Cho Saehun Kim
author_facet	Gichang Kim Mi Jang Inguk Hwang Jeongyong Cho Saehun Kim
author_sort	Gichang Kim
title	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota
title_short	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota
title_full	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota
title_fullStr	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota
title_full_unstemmed	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota
title_sort	radish sprout alleviates dss-induced colitis via regulation of nf-kb signaling pathway and modifying gut microbiota
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/049e5d36b1e74287a4415976d1b065b2
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Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota

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