Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles

Zhao Li,1,2 Iftikhar Ali,1,3 Jiying Qiu,4 Huanzhu Zhao,1 Wenya Ma,1,2 Aiying Bai,5 Daijie Wang,1 Jingchao Li1 1Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People’s Republic of China; 2College of Life Science, Shandong Norma...

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Autores principales:	Li Z, Ali I, Qiu J, Zhao H, Ma W, Bai A, Wang D, Li J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	natural flavonoid dmy-agno3-nps green approach dpph escherichia coli salmonella hela hepg2 mda-mb-231 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/04a120fc90974d2e929b903b44163355
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id	oai:doaj.org-article:04a120fc90974d2e929b903b44163355
record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN
topic	natural flavonoid dmy-agno3-nps green approach dpph escherichia coli salmonella hela hepg2 mda-mb-231 Medicine (General) R5-920
spellingShingle	natural flavonoid dmy-agno3-nps green approach dpph escherichia coli salmonella hela hepg2 mda-mb-231 Medicine (General) R5-920 Li Z Ali I Qiu J Zhao H Ma W Bai A Wang D Li J Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles
description	Zhao Li,1,2 Iftikhar Ali,1,3 Jiying Qiu,4 Huanzhu Zhao,1 Wenya Ma,1,2 Aiying Bai,5 Daijie Wang,1 Jingchao Li1 1Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People’s Republic of China; 2College of Life Science, Shandong Normal University, Jinan 250014, People’s Republic of China; 3Department of Chemistry, Karakoram International University, Gilgit 15100, Pakistan; 4Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, People’s Republic of China; 5Jinan Municipal Center for Disease Control and Prevention, Jinan 250001, People’s Republic of ChinaCorrespondence: Daijie Wang; Jingchao LiShandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People’s Republic of ChinaTel +86-53182605319Fax +86-0531-82964889Email wangdaijie@126.com; lijingchao888@sohu.comBackground: Dihydromyricetin (DMY), a natural flavonoid, has reportedly antibacterial, antioxidant, anticancer and other properties. In the present study, DMY was used as a reducing agent and stabilizer to synthesize silver nanoparticles (AgNPs), and the optimal conditions for its synthesis were studied. The DMY-AgNPs were investigated for their DPPH scavenging properties and their potential against human pathogenic and food-borne bacteria viz. Escherichia coli (E. coli), and Salmonella. In addition, DMY-AgNPs also showed excellent inhibitory effects on cancer Hela, HepG2 and MDA-MB-231 cell lines.Methods: The dihydromyricetin-mediated AgNPs (DMY-AgNPs) were characterized by ultraviolet-visible spectrophotometer (UV-Vis spectra), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD). Antioxidant activity of DMY-AgNPs was determined by 1.1-diphenyl-2-picrylhydrazyl (DPPH) scavenging. The antibacterial activity was determined by 96-well plate (AGAR) gradient dilution, while anticancer potential was determined by MTT assay.Results: The results showed that the dispersion of AgNPs had the maximum UV–visible absorption at about 410 nm. The synthesized nanoparticles were almost spherical. FTIR was used to identify functional groups that may lead to the transformation of metal ions into nanoparticles. The results showed that the prepared AgNPs were coated with biological molecules in the extraction solution. The biosynthesized DMY-AgNPs exhibited good antioxidant properties, at various concentrations (0.01– 0.1mg/mL), the free radical scavenging rate was about 56– 92%. Furthermore, DMY-AgNPs possessed good antibacterial properties against Escherichia coli (E. coli), and Salmonella at room temperature. The minimum inhibitory concentrations (MIC) were 10− 6 g/L, and 10− 4 g/L, respectively. The bioactivity of DMY-mediated AgNPs was studied using MTT assay against Hela, HepG2 and MDA-MB-231 cancer cell lines, and all showed good inhibitory effects.Conclusion: The present study provides a green approach for the synthesis of DMY-AgNPs which exhibited stronger antioxidant, antibacterial and anticancer properties compared to the dihydromyricetin. DMY-AgNPs can serve as an economical, efficient, and effective antimicrobial material for its applications in food and pharmaceutical fields.Keywords: natural flavonoid, DMY-AgNO3-NPs, green approach, DPPH, Escherichia coli, Salmonella, Hela, HepG2, MDA-MB-231
format	article
author	Li Z Ali I Qiu J Zhao H Ma W Bai A Wang D Li J
author_facet	Li Z Ali I Qiu J Zhao H Ma W Bai A Wang D Li J
author_sort	Li Z
title	Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles
title_short	Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles
title_full	Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles
title_fullStr	Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles
title_full_unstemmed	Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles
title_sort	eco-friendly and facile synthesis of antioxidant, antibacterial and anticancer dihydromyricetin-mediated silver nanoparticles
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/04a120fc90974d2e929b903b44163355
work_keys_str_mv	AT liz ecofriendlyandfacilesynthesisofantioxidantantibacterialandanticancerdihydromyricetinmediatedsilvernanoparticles AT alii ecofriendlyandfacilesynthesisofantioxidantantibacterialandanticancerdihydromyricetinmediatedsilvernanoparticles AT qiuj ecofriendlyandfacilesynthesisofantioxidantantibacterialandanticancerdihydromyricetinmediatedsilvernanoparticles AT zhaoh ecofriendlyandfacilesynthesisofantioxidantantibacterialandanticancerdihydromyricetinmediatedsilvernanoparticles AT maw ecofriendlyandfacilesynthesisofantioxidantantibacterialandanticancerdihydromyricetinmediatedsilvernanoparticles AT baia ecofriendlyandfacilesynthesisofantioxidantantibacterialandanticancerdihydromyricetinmediatedsilvernanoparticles AT wangd ecofriendlyandfacilesynthesisofantioxidantantibacterialandanticancerdihydromyricetinmediatedsilvernanoparticles AT lij ecofriendlyandfacilesynthesisofantioxidantantibacterialandanticancerdihydromyricetinmediatedsilvernanoparticles
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spelling	oai:doaj.org-article:04a120fc90974d2e929b903b441633552021-12-02T13:36:40ZEco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles1178-2013https://doaj.org/article/04a120fc90974d2e929b903b441633552021-01-01T00:00:00Zhttps://www.dovepress.com/eco-friendly-and-facile-synthesis-of-antioxidant-antibacterial-and-ant-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhao Li,1,2 Iftikhar Ali,1,3 Jiying Qiu,4 Huanzhu Zhao,1 Wenya Ma,1,2 Aiying Bai,5 Daijie Wang,1 Jingchao Li1 1Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People’s Republic of China; 2College of Life Science, Shandong Normal University, Jinan 250014, People’s Republic of China; 3Department of Chemistry, Karakoram International University, Gilgit 15100, Pakistan; 4Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, People’s Republic of China; 5Jinan Municipal Center for Disease Control and Prevention, Jinan 250001, People’s Republic of ChinaCorrespondence: Daijie Wang; Jingchao LiShandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People’s Republic of ChinaTel +86-53182605319Fax +86-0531-82964889Email wangdaijie@126.com; lijingchao888@sohu.comBackground: Dihydromyricetin (DMY), a natural flavonoid, has reportedly antibacterial, antioxidant, anticancer and other properties. In the present study, DMY was used as a reducing agent and stabilizer to synthesize silver nanoparticles (AgNPs), and the optimal conditions for its synthesis were studied. The DMY-AgNPs were investigated for their DPPH scavenging properties and their potential against human pathogenic and food-borne bacteria viz. Escherichia coli (E. coli), and Salmonella. In addition, DMY-AgNPs also showed excellent inhibitory effects on cancer Hela, HepG2 and MDA-MB-231 cell lines.Methods: The dihydromyricetin-mediated AgNPs (DMY-AgNPs) were characterized by ultraviolet-visible spectrophotometer (UV-Vis spectra), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD). Antioxidant activity of DMY-AgNPs was determined by 1.1-diphenyl-2-picrylhydrazyl (DPPH) scavenging. The antibacterial activity was determined by 96-well plate (AGAR) gradient dilution, while anticancer potential was determined by MTT assay.Results: The results showed that the dispersion of AgNPs had the maximum UV–visible absorption at about 410 nm. The synthesized nanoparticles were almost spherical. FTIR was used to identify functional groups that may lead to the transformation of metal ions into nanoparticles. The results showed that the prepared AgNPs were coated with biological molecules in the extraction solution. The biosynthesized DMY-AgNPs exhibited good antioxidant properties, at various concentrations (0.01– 0.1mg/mL), the free radical scavenging rate was about 56– 92%. Furthermore, DMY-AgNPs possessed good antibacterial properties against Escherichia coli (E. coli), and Salmonella at room temperature. The minimum inhibitory concentrations (MIC) were 10− 6 g/L, and 10− 4 g/L, respectively. The bioactivity of DMY-mediated AgNPs was studied using MTT assay against Hela, HepG2 and MDA-MB-231 cancer cell lines, and all showed good inhibitory effects.Conclusion: The present study provides a green approach for the synthesis of DMY-AgNPs which exhibited stronger antioxidant, antibacterial and anticancer properties compared to the dihydromyricetin. DMY-AgNPs can serve as an economical, efficient, and effective antimicrobial material for its applications in food and pharmaceutical fields.Keywords: natural flavonoid, DMY-AgNO3-NPs, green approach, DPPH, Escherichia coli, Salmonella, Hela, HepG2, MDA-MB-231Li ZAli IQiu JZhao HMa WBai AWang DLi JDove Medical Pressarticlenatural flavonoiddmy-agno3-npsgreen approachdpphescherichia colisalmonellahelahepg2mda-mb-231Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 481-492 (2021)

Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles

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