Networked Chemoreceptors Benefit Bacterial Chemotaxis Performance

ABSTRACT Motile bacteria use large receptor arrays to detect and follow chemical gradients in their environment. Extended receptor arrays, composed of networked signaling complexes, promote cooperative stimulus control of their associated signaling kinases. Here, we used structural lesions at the co...

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Autores principales:	Vered Frank, Germán E. Piñas, Harel Cohen, John S. Parkinson, Ady Vaknin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/04a381a884df4d06b35eeb0e2a3c6f9e
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spelling	oai:doaj.org-article:04a381a884df4d06b35eeb0e2a3c6f9e2021-11-15T15:50:16ZNetworked Chemoreceptors Benefit Bacterial Chemotaxis Performance10.1128/mBio.01824-162150-7511https://doaj.org/article/04a381a884df4d06b35eeb0e2a3c6f9e2016-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01824-16https://doaj.org/toc/2150-7511ABSTRACT Motile bacteria use large receptor arrays to detect and follow chemical gradients in their environment. Extended receptor arrays, composed of networked signaling complexes, promote cooperative stimulus control of their associated signaling kinases. Here, we used structural lesions at the communication interface between core complexes to create an Escherichia coli strain with functional but dispersed signaling complexes. This strain allowed us to directly study how networking of signaling complexes affects chemotactic signaling and gradient-tracking performance. We demonstrate that networking of receptor complexes provides bacterial cells with about 10-fold-heightened detection sensitivity to attractants while maintaining a wide dynamic range over which receptor adaptational modifications can tune response sensitivity. These advantages proved especially critical for chemotaxis toward an attractant source under conditions in which bacteria are unable to alter the attractant gradient. IMPORTANCE Chemoreceptor arrays are found in many motile bacteria. However, although our understanding of bacterial chemotaxis is quite detailed, the signaling and behavioral advantages of networked receptor arrays had not been directly studied in cells. We have recently shown that lesions in a key interface of the E. coli receptor array diminish physical connections and functional coupling between core signaling complexes while maintaining their basic signaling capacity. In this study, we exploited an interface 2 mutant to show, for the first time, that coupling between core complexes substantially enhances stimulus detection and chemotaxis performance.Vered FrankGermán E. PiñasHarel CohenJohn S. ParkinsonAdy VakninAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 6 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Vered Frank Germán E. Piñas Harel Cohen John S. Parkinson Ady Vaknin Networked Chemoreceptors Benefit Bacterial Chemotaxis Performance
description	ABSTRACT Motile bacteria use large receptor arrays to detect and follow chemical gradients in their environment. Extended receptor arrays, composed of networked signaling complexes, promote cooperative stimulus control of their associated signaling kinases. Here, we used structural lesions at the communication interface between core complexes to create an Escherichia coli strain with functional but dispersed signaling complexes. This strain allowed us to directly study how networking of signaling complexes affects chemotactic signaling and gradient-tracking performance. We demonstrate that networking of receptor complexes provides bacterial cells with about 10-fold-heightened detection sensitivity to attractants while maintaining a wide dynamic range over which receptor adaptational modifications can tune response sensitivity. These advantages proved especially critical for chemotaxis toward an attractant source under conditions in which bacteria are unable to alter the attractant gradient. IMPORTANCE Chemoreceptor arrays are found in many motile bacteria. However, although our understanding of bacterial chemotaxis is quite detailed, the signaling and behavioral advantages of networked receptor arrays had not been directly studied in cells. We have recently shown that lesions in a key interface of the E. coli receptor array diminish physical connections and functional coupling between core signaling complexes while maintaining their basic signaling capacity. In this study, we exploited an interface 2 mutant to show, for the first time, that coupling between core complexes substantially enhances stimulus detection and chemotaxis performance.
format	article
author	Vered Frank Germán E. Piñas Harel Cohen John S. Parkinson Ady Vaknin
author_facet	Vered Frank Germán E. Piñas Harel Cohen John S. Parkinson Ady Vaknin
author_sort	Vered Frank
title	Networked Chemoreceptors Benefit Bacterial Chemotaxis Performance
title_short	Networked Chemoreceptors Benefit Bacterial Chemotaxis Performance
title_full	Networked Chemoreceptors Benefit Bacterial Chemotaxis Performance
title_fullStr	Networked Chemoreceptors Benefit Bacterial Chemotaxis Performance
title_full_unstemmed	Networked Chemoreceptors Benefit Bacterial Chemotaxis Performance
title_sort	networked chemoreceptors benefit bacterial chemotaxis performance
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/04a381a884df4d06b35eeb0e2a3c6f9e
work_keys_str_mv	AT veredfrank networkedchemoreceptorsbenefitbacterialchemotaxisperformance AT germanepinas networkedchemoreceptorsbenefitbacterialchemotaxisperformance AT harelcohen networkedchemoreceptorsbenefitbacterialchemotaxisperformance AT johnsparkinson networkedchemoreceptorsbenefitbacterialchemotaxisperformance AT adyvaknin networkedchemoreceptorsbenefitbacterialchemotaxisperformance
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Networked Chemoreceptors Benefit Bacterial Chemotaxis Performance

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