Benzyl Isothiocyanate, a Vegetable-Derived Compound, Induces Apoptosis via ROS Accumulation and DNA Damage in Canine Lymphoma and Leukemia Cells

Treatment of neoplastic diseases in companion animals is one of the most important problems of modern veterinary medicine. Given the growing interest in substances of natural origin as potential anti-cancer drugs, our goal was to examine the effectiveness of benzyl isothiocyanate (BITC), a compound...

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Autores principales:	Marta Henklewska, Aleksandra Pawlak, Rong-Fang Li, Jine Yi, Iwona Zbyryt, Bożena Obmińska-Mrukowicz
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	BITC anti-cancer drugs canine cancers cancer treatment oxidative stress Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/04aa656ac79846899bcaf02fe3a6b849
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spelling	oai:doaj.org-article:04aa656ac79846899bcaf02fe3a6b8492021-11-11T17:13:33ZBenzyl Isothiocyanate, a Vegetable-Derived Compound, Induces Apoptosis via ROS Accumulation and DNA Damage in Canine Lymphoma and Leukemia Cells10.3390/ijms2221117721422-00671661-6596https://doaj.org/article/04aa656ac79846899bcaf02fe3a6b8492021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11772https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Treatment of neoplastic diseases in companion animals is one of the most important problems of modern veterinary medicine. Given the growing interest in substances of natural origin as potential anti-cancer drugs, our goal was to examine the effectiveness of benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables, against canine lymphoma and leukemia. These are the one of the most common canine cancer types, and chemotherapy is the only treatment option. The study involved established cell lines originating from various hematopoietic malignancies: CLBL-1, GL-1, CLB70 and CNK-89, immortalized noncancerous cell lines: MDCK and NIH-3T3 and canine peripheral blood mononuclear cells (PBMCs). The cytotoxic activity of BITC, apoptosis induction, caspase activity and ROS generation were evaluated by flow cytometry. H2AX phosphorylation was assessed by western blot. The study showed that the compound was especially active against B lymphocyte-derived malignant cells. Their death resulted from caspase-dependent apoptosis. BITC induced ROS accumulation, and glutathione precursor N-acetyl-l-cysteine reversed the effect of the compound, thus proving the role of oxidative stress in BITC activity. In addition, exposure to the compound induced DNA damage in the tested cells. This is the first study that provides information on the activity of BITC in canine hematopoietic malignancies and suggests that the compound may be particularly useful in B-cell neoplasms treatment.Marta HenklewskaAleksandra PawlakRong-Fang LiJine YiIwona ZbyrytBożena Obmińska-MrukowiczMDPI AGarticleBITCanti-cancer drugscanine cancerscancer treatmentoxidative stressBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11772, p 11772 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	BITC anti-cancer drugs canine cancers cancer treatment oxidative stress Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	BITC anti-cancer drugs canine cancers cancer treatment oxidative stress Biology (General) QH301-705.5 Chemistry QD1-999 Marta Henklewska Aleksandra Pawlak Rong-Fang Li Jine Yi Iwona Zbyryt Bożena Obmińska-Mrukowicz Benzyl Isothiocyanate, a Vegetable-Derived Compound, Induces Apoptosis via ROS Accumulation and DNA Damage in Canine Lymphoma and Leukemia Cells
description	Treatment of neoplastic diseases in companion animals is one of the most important problems of modern veterinary medicine. Given the growing interest in substances of natural origin as potential anti-cancer drugs, our goal was to examine the effectiveness of benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables, against canine lymphoma and leukemia. These are the one of the most common canine cancer types, and chemotherapy is the only treatment option. The study involved established cell lines originating from various hematopoietic malignancies: CLBL-1, GL-1, CLB70 and CNK-89, immortalized noncancerous cell lines: MDCK and NIH-3T3 and canine peripheral blood mononuclear cells (PBMCs). The cytotoxic activity of BITC, apoptosis induction, caspase activity and ROS generation were evaluated by flow cytometry. H2AX phosphorylation was assessed by western blot. The study showed that the compound was especially active against B lymphocyte-derived malignant cells. Their death resulted from caspase-dependent apoptosis. BITC induced ROS accumulation, and glutathione precursor N-acetyl-l-cysteine reversed the effect of the compound, thus proving the role of oxidative stress in BITC activity. In addition, exposure to the compound induced DNA damage in the tested cells. This is the first study that provides information on the activity of BITC in canine hematopoietic malignancies and suggests that the compound may be particularly useful in B-cell neoplasms treatment.
format	article
author	Marta Henklewska Aleksandra Pawlak Rong-Fang Li Jine Yi Iwona Zbyryt Bożena Obmińska-Mrukowicz
author_facet	Marta Henklewska Aleksandra Pawlak Rong-Fang Li Jine Yi Iwona Zbyryt Bożena Obmińska-Mrukowicz
author_sort	Marta Henklewska
title	Benzyl Isothiocyanate, a Vegetable-Derived Compound, Induces Apoptosis via ROS Accumulation and DNA Damage in Canine Lymphoma and Leukemia Cells
title_short	Benzyl Isothiocyanate, a Vegetable-Derived Compound, Induces Apoptosis via ROS Accumulation and DNA Damage in Canine Lymphoma and Leukemia Cells
title_full	Benzyl Isothiocyanate, a Vegetable-Derived Compound, Induces Apoptosis via ROS Accumulation and DNA Damage in Canine Lymphoma and Leukemia Cells
title_fullStr	Benzyl Isothiocyanate, a Vegetable-Derived Compound, Induces Apoptosis via ROS Accumulation and DNA Damage in Canine Lymphoma and Leukemia Cells
title_full_unstemmed	Benzyl Isothiocyanate, a Vegetable-Derived Compound, Induces Apoptosis via ROS Accumulation and DNA Damage in Canine Lymphoma and Leukemia Cells
title_sort	benzyl isothiocyanate, a vegetable-derived compound, induces apoptosis via ros accumulation and dna damage in canine lymphoma and leukemia cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/04aa656ac79846899bcaf02fe3a6b849
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Benzyl Isothiocyanate, a Vegetable-Derived Compound, Induces Apoptosis via ROS Accumulation and DNA Damage in Canine Lymphoma and Leukemia Cells

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