Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM
Here, the authors present cryoEMPEM, a method for high-resolution structural analysis of vaccine-elicited polyclonal antibody responses. They apply cryoEMPEM in combination with standard serology experiments to characterize the polyclonal antibody (pAb) responses elicited in rhesus macaques by HIV E...
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Nature Portfolio
2021
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oai:doaj.org-article:04ab87746dbe4d4ea81ab039e453c3ac2021-12-02T16:43:43ZPolyclonal antibody responses to HIV Env immunogens resolved using cryoEM10.1038/s41467-021-25087-42041-1723https://doaj.org/article/04ab87746dbe4d4ea81ab039e453c3ac2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25087-4https://doaj.org/toc/2041-1723Here, the authors present cryoEMPEM, a method for high-resolution structural analysis of vaccine-elicited polyclonal antibody responses. They apply cryoEMPEM in combination with standard serology experiments to characterize the polyclonal antibody (pAb) responses elicited in rhesus macaques by HIV Env trimer immunogens and were able to determine up to 8 different polyclonal antibody structures in complex with their respective antigen from a single cryoEM dataset.Aleksandar AntanasijevicLeigh M. SewallChristopher A. CottrellDiane G. CarnathanLuis E. JimenezJulia T. NgoJennifer B. SilvermanBettina GroschelErik GeorgesonJinal BhimanRaiza BastidasCelia LaBrancheJoel D. AllenJeffrey CoppsHailee R. PerrettKimmo RantalainenFabien CannacYuhe R. YangAlba Torrents de la PeñaRebeca Froes RochaZachary T. BerndsenDavid BakerNeil P. KingRogier W. SandersJohn P. MooreShane CrottyMax CrispinDavid C. MontefioriDennis R. BurtonWilliam R. SchiefGuido SilvestriAndrew B. WardNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-17 (2021) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Science Q |
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Science Q Aleksandar Antanasijevic Leigh M. Sewall Christopher A. Cottrell Diane G. Carnathan Luis E. Jimenez Julia T. Ngo Jennifer B. Silverman Bettina Groschel Erik Georgeson Jinal Bhiman Raiza Bastidas Celia LaBranche Joel D. Allen Jeffrey Copps Hailee R. Perrett Kimmo Rantalainen Fabien Cannac Yuhe R. Yang Alba Torrents de la Peña Rebeca Froes Rocha Zachary T. Berndsen David Baker Neil P. King Rogier W. Sanders John P. Moore Shane Crotty Max Crispin David C. Montefiori Dennis R. Burton William R. Schief Guido Silvestri Andrew B. Ward Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM |
| description |
Here, the authors present cryoEMPEM, a method for high-resolution structural analysis of vaccine-elicited polyclonal antibody responses. They apply cryoEMPEM in combination with standard serology experiments to characterize the polyclonal antibody (pAb) responses elicited in rhesus macaques by HIV Env trimer immunogens and were able to determine up to 8 different polyclonal antibody structures in complex with their respective antigen from a single cryoEM dataset. |
| format |
article |
| author |
Aleksandar Antanasijevic Leigh M. Sewall Christopher A. Cottrell Diane G. Carnathan Luis E. Jimenez Julia T. Ngo Jennifer B. Silverman Bettina Groschel Erik Georgeson Jinal Bhiman Raiza Bastidas Celia LaBranche Joel D. Allen Jeffrey Copps Hailee R. Perrett Kimmo Rantalainen Fabien Cannac Yuhe R. Yang Alba Torrents de la Peña Rebeca Froes Rocha Zachary T. Berndsen David Baker Neil P. King Rogier W. Sanders John P. Moore Shane Crotty Max Crispin David C. Montefiori Dennis R. Burton William R. Schief Guido Silvestri Andrew B. Ward |
| author_facet |
Aleksandar Antanasijevic Leigh M. Sewall Christopher A. Cottrell Diane G. Carnathan Luis E. Jimenez Julia T. Ngo Jennifer B. Silverman Bettina Groschel Erik Georgeson Jinal Bhiman Raiza Bastidas Celia LaBranche Joel D. Allen Jeffrey Copps Hailee R. Perrett Kimmo Rantalainen Fabien Cannac Yuhe R. Yang Alba Torrents de la Peña Rebeca Froes Rocha Zachary T. Berndsen David Baker Neil P. King Rogier W. Sanders John P. Moore Shane Crotty Max Crispin David C. Montefiori Dennis R. Burton William R. Schief Guido Silvestri Andrew B. Ward |
| author_sort |
Aleksandar Antanasijevic |
| title |
Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM |
| title_short |
Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM |
| title_full |
Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM |
| title_fullStr |
Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM |
| title_full_unstemmed |
Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM |
| title_sort |
polyclonal antibody responses to hiv env immunogens resolved using cryoem |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/04ab87746dbe4d4ea81ab039e453c3ac |
| work_keys_str_mv |
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