Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells

Adipose-derived stem cells (ASCs) are an abundant and easily accessible multipotent stem cell source with potential application in smooth muscle regeneration strategies. In 3D collagen hydrogels, we investigated whether sustained release of growth factors (GF) PDGF-AB and TGF-β1 from GF-loaded micro...

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Autores principales:	Brandan Walters, Paul A. Turner, Bernd Rolauffs, Melanie L. Hart, Jan P. Stegemann
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adipose-derived stem cells smooth muscle tissue engineering microspheres growth factors cyclic stretch cell morphology Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/04ac1f8d1d3446ceae49ec0940ec0f8a
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spelling	oai:doaj.org-article:04ac1f8d1d3446ceae49ec0940ec0f8a2021-11-25T17:11:44ZControlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells10.3390/cells101131232073-4409https://doaj.org/article/04ac1f8d1d3446ceae49ec0940ec0f8a2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3123https://doaj.org/toc/2073-4409Adipose-derived stem cells (ASCs) are an abundant and easily accessible multipotent stem cell source with potential application in smooth muscle regeneration strategies. In 3D collagen hydrogels, we investigated whether sustained release of growth factors (GF) PDGF-AB and TGF-β1 from GF-loaded microspheres could induce a smooth muscle cell (SMC) phenotype in ASCs, and if the addition of uniaxial cyclic stretch could enhance the differentiation level. This study demonstrated that the combination of cyclic stretch and GF release over time from loaded microspheres potentiated the differentiation of ASCs, as quantified by protein expression of early to late SMC differentiation markers (SMA, TGLN and smooth muscle MHC). The delivery of GFs via microspheres produced large ASCs with a spindle-shaped, elongated SMC-like morphology. Cyclic strain produced the largest, longest, and most spindle-shaped cells regardless of the presence or absence of growth factors or the growth factor delivery method. Protein expression and cell morphology data confirmed that the sustained release of GFs from GF-loaded microspheres can be used to promote the differentiation of ASCs into SMCs and that the addition of uniaxial cyclic stretch significantly enhances the differentiation level, as quantified by intermediate and late SMC markers and a SMC-like elongated cell morphology.Brandan WaltersPaul A. TurnerBernd RolauffsMelanie L. HartJan P. StegemannMDPI AGarticleadipose-derived stem cellssmooth muscle tissue engineeringmicrospheresgrowth factorscyclic stretchcell morphologyBiology (General)QH301-705.5ENCells, Vol 10, Iss 3123, p 3123 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipose-derived stem cells smooth muscle tissue engineering microspheres growth factors cyclic stretch cell morphology Biology (General) QH301-705.5
spellingShingle	adipose-derived stem cells smooth muscle tissue engineering microspheres growth factors cyclic stretch cell morphology Biology (General) QH301-705.5 Brandan Walters Paul A. Turner Bernd Rolauffs Melanie L. Hart Jan P. Stegemann Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells
description	Adipose-derived stem cells (ASCs) are an abundant and easily accessible multipotent stem cell source with potential application in smooth muscle regeneration strategies. In 3D collagen hydrogels, we investigated whether sustained release of growth factors (GF) PDGF-AB and TGF-β1 from GF-loaded microspheres could induce a smooth muscle cell (SMC) phenotype in ASCs, and if the addition of uniaxial cyclic stretch could enhance the differentiation level. This study demonstrated that the combination of cyclic stretch and GF release over time from loaded microspheres potentiated the differentiation of ASCs, as quantified by protein expression of early to late SMC differentiation markers (SMA, TGLN and smooth muscle MHC). The delivery of GFs via microspheres produced large ASCs with a spindle-shaped, elongated SMC-like morphology. Cyclic strain produced the largest, longest, and most spindle-shaped cells regardless of the presence or absence of growth factors or the growth factor delivery method. Protein expression and cell morphology data confirmed that the sustained release of GFs from GF-loaded microspheres can be used to promote the differentiation of ASCs into SMCs and that the addition of uniaxial cyclic stretch significantly enhances the differentiation level, as quantified by intermediate and late SMC markers and a SMC-like elongated cell morphology.
format	article
author	Brandan Walters Paul A. Turner Bernd Rolauffs Melanie L. Hart Jan P. Stegemann
author_facet	Brandan Walters Paul A. Turner Bernd Rolauffs Melanie L. Hart Jan P. Stegemann
author_sort	Brandan Walters
title	Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells
title_short	Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells
title_full	Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells
title_fullStr	Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells
title_full_unstemmed	Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells
title_sort	controlled growth factor delivery and cyclic stretch induces a smooth muscle cell-like phenotype in adipose-derived stem cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/04ac1f8d1d3446ceae49ec0940ec0f8a
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Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells

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