Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

Introduction. We aimed to explore the downregulation of the coiled-coil domain containing 80 (CCDC80) and its underlying molecular mechanisms in ovarian carcinoma (OVCA). Materials/Methods. Immunohistochemical staining was performed to confirm the expression status of CCDC80 protein. Combining the d...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zi-Qian Liang, Li Gao, Jun-Hong Chen, Wen-Bin Dai, Ya-Si Su, Gang Chen
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/04c8b90e54aa44d0bce95ea2575009b5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:04c8b90e54aa44d0bce95ea2575009b5
record_format	dspace
spelling	oai:doaj.org-article:04c8b90e54aa44d0bce95ea2575009b52021-11-29T00:57:07ZDownregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study2314-437810.1155/2021/3752871https://doaj.org/article/04c8b90e54aa44d0bce95ea2575009b52021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/3752871https://doaj.org/toc/2314-4378Introduction. We aimed to explore the downregulation of the coiled-coil domain containing 80 (CCDC80) and its underlying molecular mechanisms in ovarian carcinoma (OVCA). Materials/Methods. Immunohistochemical staining was performed to confirm the expression status of CCDC80 protein. Combining the data from in-house tissue microarrays and high-throughput datasets, we identified the expression level of CCDC80 in OVCA. We utilized cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) algorithm and single-sample gene set enrichment analysis (ssGSEA) to explore the relationship between CCDC80 and the tumor microenvironment (TME) landscape in OVCA. Pathway enrichment, function annotation, and transcription factor (TFs) exploration were conducted to study the latent molecular mechanisms. Moreover, the cell line data in the Genomics of Drug Sensitivity in Cancer (GDSC) database was used to discover the relationship between CCDC80 and drug sensitivity. Results. An integrated standard mean difference (SMD) of −0.919 (95% CI: −1.515–0.324, P=0.002) identified the downregulation of CCDC80 in OVCA based on 1048 samples, and the sROC (AUC=0.76) showed a moderate discriminatory ability of CCDC80 in OVCA. The fraction of infiltrating naive B cells showed significant differences between the high- and low-CCDC80 expression groups. Also, CCDC80-related genes are enriched in the Ras signaling pathway and metabolic of lipid. Nuclear receptor subfamily three group C member 1 (NR3C1) may be an upstream TF of CCDC80, and CCDC80 may be related to the sensitivity of mitocycin C and nilotinib. Conclusion. CCDC80 was downregulated in OVCA and may play a role as a tumor suppressor in OVCA.Zi-Qian LiangLi GaoJun-Hong ChenWen-Bin DaiYa-Si SuGang ChenHindawi LimitedarticleGeneticsQH426-470ENInternational Journal of Genomics, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Zi-Qian Liang Li Gao Jun-Hong Chen Wen-Bin Dai Ya-Si Su Gang Chen Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study
description	Introduction. We aimed to explore the downregulation of the coiled-coil domain containing 80 (CCDC80) and its underlying molecular mechanisms in ovarian carcinoma (OVCA). Materials/Methods. Immunohistochemical staining was performed to confirm the expression status of CCDC80 protein. Combining the data from in-house tissue microarrays and high-throughput datasets, we identified the expression level of CCDC80 in OVCA. We utilized cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) algorithm and single-sample gene set enrichment analysis (ssGSEA) to explore the relationship between CCDC80 and the tumor microenvironment (TME) landscape in OVCA. Pathway enrichment, function annotation, and transcription factor (TFs) exploration were conducted to study the latent molecular mechanisms. Moreover, the cell line data in the Genomics of Drug Sensitivity in Cancer (GDSC) database was used to discover the relationship between CCDC80 and drug sensitivity. Results. An integrated standard mean difference (SMD) of −0.919 (95% CI: −1.515–0.324, P=0.002) identified the downregulation of CCDC80 in OVCA based on 1048 samples, and the sROC (AUC=0.76) showed a moderate discriminatory ability of CCDC80 in OVCA. The fraction of infiltrating naive B cells showed significant differences between the high- and low-CCDC80 expression groups. Also, CCDC80-related genes are enriched in the Ras signaling pathway and metabolic of lipid. Nuclear receptor subfamily three group C member 1 (NR3C1) may be an upstream TF of CCDC80, and CCDC80 may be related to the sensitivity of mitocycin C and nilotinib. Conclusion. CCDC80 was downregulated in OVCA and may play a role as a tumor suppressor in OVCA.
format	article
author	Zi-Qian Liang Li Gao Jun-Hong Chen Wen-Bin Dai Ya-Si Su Gang Chen
author_facet	Zi-Qian Liang Li Gao Jun-Hong Chen Wen-Bin Dai Ya-Si Su Gang Chen
author_sort	Zi-Qian Liang
title	Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study
title_short	Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study
title_full	Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study
title_fullStr	Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study
title_full_unstemmed	Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study
title_sort	downregulation of the coiled-coil domain containing 80 and its perspective mechanisms in ovarian carcinoma: a comprehensive study
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/04c8b90e54aa44d0bce95ea2575009b5
work_keys_str_mv	AT ziqianliang downregulationofthecoiledcoildomaincontaining80anditsperspectivemechanismsinovariancarcinomaacomprehensivestudy AT ligao downregulationofthecoiledcoildomaincontaining80anditsperspectivemechanismsinovariancarcinomaacomprehensivestudy AT junhongchen downregulationofthecoiledcoildomaincontaining80anditsperspectivemechanismsinovariancarcinomaacomprehensivestudy AT wenbindai downregulationofthecoiledcoildomaincontaining80anditsperspectivemechanismsinovariancarcinomaacomprehensivestudy AT yasisu downregulationofthecoiledcoildomaincontaining80anditsperspectivemechanismsinovariancarcinomaacomprehensivestudy AT gangchen downregulationofthecoiledcoildomaincontaining80anditsperspectivemechanismsinovariancarcinomaacomprehensivestudy
_version_	1718407627794284544

Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

Ejemplares similares