The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep

This experiment was to study the impact of rumen fistula surgery on the rumen microbios in sheep. Six male adult Hu sheep (48.8 ± 0.23 kg, 0.5 years) were fed at 0700 and 1,800 with ad libitum access to water. The rumen fistula was installed in the same batch from 0600 to 0900. Monitoring the dry ma...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yurong Cao, Baozhen Zhu, Fei Li, Duihong Zhang, Tongqing Guo, Fadi Li, Guo Yang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	rumen fistula surgery microbial diversity sheep rumen microbial function BugBase function prediction Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/04ce2f7c98c04934878c7ecffc21d935
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:04ce2f7c98c04934878c7ecffc21d935
record_format	dspace
spelling	oai:doaj.org-article:04ce2f7c98c04934878c7ecffc21d9352021-12-01T22:14:38ZThe Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep2297-176910.3389/fvets.2021.754195https://doaj.org/article/04ce2f7c98c04934878c7ecffc21d9352021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.754195/fullhttps://doaj.org/toc/2297-1769This experiment was to study the impact of rumen fistula surgery on the rumen microbios in sheep. Six male adult Hu sheep (48.8 ± 0.23 kg, 0.5 years) were fed at 0700 and 1,800 with ad libitum access to water. The rumen fistula was installed in the same batch from 0600 to 0900. Monitoring the dry mater intake and the output of dry mater faces 1, 2, 4, 6, 8, 10, 12, 14 days after fistulated surgery. The collection of rumen fluid was arranged at 1d during rumen surgery (DRS1), 3d after rumen surgery (ARS3), and 14d after rumen surgery (ARS14) for volatile fatty acid (VFA) and DNA extraction for sequencing and bioinformatics analysis. There was no difference in DMI, the pH apparent digestibility of dry matter, organic matter, neutral detergent fiber, acid detergent fiber both before and 14 days after surgery. Increases were observed in the acetate and total VFA at ARS3. There was no difference in digestion of dry material, organic material, neutral detergent fiber, and acid detergent fiber before and after surgery. The relative abundance of Bacteroides decreased from 61.96% at DRS1 to 28.85% at ARS3. In comparison with the DRS1 and ARS3, the relative abundance of Firmicutes in the ARS14 increased to 44.58% (P < 0.01). Proteobacteria increased from 11.33% at DRS1 to 51.66% at ARS3 and then decreased to 11.39% at ARS14. Prevotella decreased form 61.06% at DRS1 to 28.04% in the ARS3. Succinivibrio increased from 8.32% at DRS1 to 48.58% at ARS3, but decreased to 10.43% in the ARS14. Compared with DRS1 and ARS3, the ARS14 was higher in the Simpson and Shannon index. As for the BugBase function prediction, rumen fistula surgery increased the microorganism abundance of aerobic and facultative anaerobic phenotype, and anaerobic phenotype was decreased in the ARS3. There was higher microorganism abundance of aerobic phenotype in the ARS14 than before fistula installation. In conclusion, the rumen fistula surgery destroys the anaerobic environment of rumen, leading to differences in rumen microbial diversity and function, but the apparent digestibility and total VFA were not affected.Yurong CaoBaozhen ZhuFei LiDuihong ZhangTongqing GuoFadi LiGuo YangFrontiers Media S.A.articlerumen fistula surgerymicrobial diversitysheeprumen microbial functionBugBase function predictionVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rumen fistula surgery microbial diversity sheep rumen microbial function BugBase function prediction Veterinary medicine SF600-1100
spellingShingle	rumen fistula surgery microbial diversity sheep rumen microbial function BugBase function prediction Veterinary medicine SF600-1100 Yurong Cao Baozhen Zhu Fei Li Duihong Zhang Tongqing Guo Fadi Li Guo Yang The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep
description	This experiment was to study the impact of rumen fistula surgery on the rumen microbios in sheep. Six male adult Hu sheep (48.8 ± 0.23 kg, 0.5 years) were fed at 0700 and 1,800 with ad libitum access to water. The rumen fistula was installed in the same batch from 0600 to 0900. Monitoring the dry mater intake and the output of dry mater faces 1, 2, 4, 6, 8, 10, 12, 14 days after fistulated surgery. The collection of rumen fluid was arranged at 1d during rumen surgery (DRS1), 3d after rumen surgery (ARS3), and 14d after rumen surgery (ARS14) for volatile fatty acid (VFA) and DNA extraction for sequencing and bioinformatics analysis. There was no difference in DMI, the pH apparent digestibility of dry matter, organic matter, neutral detergent fiber, acid detergent fiber both before and 14 days after surgery. Increases were observed in the acetate and total VFA at ARS3. There was no difference in digestion of dry material, organic material, neutral detergent fiber, and acid detergent fiber before and after surgery. The relative abundance of Bacteroides decreased from 61.96% at DRS1 to 28.85% at ARS3. In comparison with the DRS1 and ARS3, the relative abundance of Firmicutes in the ARS14 increased to 44.58% (P < 0.01). Proteobacteria increased from 11.33% at DRS1 to 51.66% at ARS3 and then decreased to 11.39% at ARS14. Prevotella decreased form 61.06% at DRS1 to 28.04% in the ARS3. Succinivibrio increased from 8.32% at DRS1 to 48.58% at ARS3, but decreased to 10.43% in the ARS14. Compared with DRS1 and ARS3, the ARS14 was higher in the Simpson and Shannon index. As for the BugBase function prediction, rumen fistula surgery increased the microorganism abundance of aerobic and facultative anaerobic phenotype, and anaerobic phenotype was decreased in the ARS3. There was higher microorganism abundance of aerobic phenotype in the ARS14 than before fistula installation. In conclusion, the rumen fistula surgery destroys the anaerobic environment of rumen, leading to differences in rumen microbial diversity and function, but the apparent digestibility and total VFA were not affected.
format	article
author	Yurong Cao Baozhen Zhu Fei Li Duihong Zhang Tongqing Guo Fadi Li Guo Yang
author_facet	Yurong Cao Baozhen Zhu Fei Li Duihong Zhang Tongqing Guo Fadi Li Guo Yang
author_sort	Yurong Cao
title	The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep
title_short	The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep
title_full	The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep
title_fullStr	The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep
title_full_unstemmed	The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep
title_sort	destruction of the anaerobic environment caused by rumen fistula surgery leads to differences in the rumen microbial diversity and function of sheep
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/04ce2f7c98c04934878c7ecffc21d935
work_keys_str_mv	AT yurongcao thedestructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT baozhenzhu thedestructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT feili thedestructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT duihongzhang thedestructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT tongqingguo thedestructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT fadili thedestructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT guoyang thedestructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT yurongcao destructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT baozhenzhu destructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT feili destructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT duihongzhang destructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT tongqingguo destructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT fadili destructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep AT guoyang destructionoftheanaerobicenvironmentcausedbyrumenfistulasurgeryleadstodifferencesintherumenmicrobialdiversityandfunctionofsheep
_version_	1718404142116896768

The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep

Ejemplares similares