The synergistic radiosensitizing effect of tirapazamine-conjugated gold nanoparticles on human hepatoma HepG2 cells under X-ray irradiation
Xi Liu,1–4 Yan Liu,1–4 Pengcheng Zhang,1–4 Xiaodong Jin,1–3 Xiaogang Zheng,1–4 Fei Ye,1–4 Weiqiang Chen,1–3 Qiang Li1–3 1Institute of Modern Physics, 2Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2016
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/04e081637f274b519080812d5434a312 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:04e081637f274b519080812d5434a312 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:04e081637f274b519080812d5434a3122021-12-02T04:03:04ZThe synergistic radiosensitizing effect of tirapazamine-conjugated gold nanoparticles on human hepatoma HepG2 cells under X-ray irradiation1178-2013https://doaj.org/article/04e081637f274b519080812d5434a3122016-07-01T00:00:00Zhttps://www.dovepress.com/the-synergistic-radiosensitizing-effect-of-tirapazamine-conjugated-gol-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xi Liu,1–4 Yan Liu,1–4 Pengcheng Zhang,1–4 Xiaodong Jin,1–3 Xiaogang Zheng,1–4 Fei Ye,1–4 Weiqiang Chen,1–3 Qiang Li1–3 1Institute of Modern Physics, 2Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, 3Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Gansu Province, Lanzhou, 4School of Life Science, University of Chinese Academy of Sciences, Beijing, People’s Republic of China Abstract: Reductive drug-functionalized gold nanoparticles (AuNPs) have been proposed to enhance the damage of X-rays to cells through improving hydroxyl radical production by secondary electrons. In this work, polyethylene glycol-capped AuNPs were conjugated with tirapazamine (TPZ) moiety, and then thioctyl TPZ (TPZs)-modified AuNPs (TPZs-AuNPs) were synthesized. The TPZs-AuNPs were characterized by transmission electron microscopy, ultraviolet-visible spectra, dynamic light scattering, and inductively coupled plasma mass spectrometry to have a size of 16.6±2.1 nm in diameter and a TPZs/AuNPs ratio of ~700:1. In contrast with PEGylated AuNPs, the as-synthesized TPZs-AuNPs exhibited 20% increment in hydroxyl radical production in water at 2.0 Gy, and 19% increase in sensitizer enhancement ratio at 10% survival fraction for human hepatoma HepG2 cells under X-ray irradiation. The production of reactive oxygen species in HepG2 cells exposed to X-rays in vitro demonstrated a synergistic radiosensitizing effect of AuNPs and TPZ moiety. Thus, the reductive drug-conjugated TPZs-AuNPs as a kind of AuNP radiosensitizer with low gold loading provide a new strategy for enhancing the efficacy of radiation therapy. Keywords: AuNPs, radiation enhancement, synergistic effect, human hepatoma cells, hydroxyl radical productionLiu XLiu YZhang PJin XZheng XYe FChen WLi QDove Medical Pressarticlegold nanoparticles (AuNPs)radiation enhancementsynergistic effecthuman hepatoma cellshydroxyl radical productionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 3517-3531 (2016) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
gold nanoparticles (AuNPs) radiation enhancement synergistic effect human hepatoma cells hydroxyl radical production Medicine (General) R5-920 |
| spellingShingle |
gold nanoparticles (AuNPs) radiation enhancement synergistic effect human hepatoma cells hydroxyl radical production Medicine (General) R5-920 Liu X Liu Y Zhang P Jin X Zheng X Ye F Chen W Li Q The synergistic radiosensitizing effect of tirapazamine-conjugated gold nanoparticles on human hepatoma HepG2 cells under X-ray irradiation |
| description |
Xi Liu,1–4 Yan Liu,1–4 Pengcheng Zhang,1–4 Xiaodong Jin,1–3 Xiaogang Zheng,1–4 Fei Ye,1–4 Weiqiang Chen,1–3 Qiang Li1–3 1Institute of Modern Physics, 2Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, 3Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Gansu Province, Lanzhou, 4School of Life Science, University of Chinese Academy of Sciences, Beijing, People’s Republic of China Abstract: Reductive drug-functionalized gold nanoparticles (AuNPs) have been proposed to enhance the damage of X-rays to cells through improving hydroxyl radical production by secondary electrons. In this work, polyethylene glycol-capped AuNPs were conjugated with tirapazamine (TPZ) moiety, and then thioctyl TPZ (TPZs)-modified AuNPs (TPZs-AuNPs) were synthesized. The TPZs-AuNPs were characterized by transmission electron microscopy, ultraviolet-visible spectra, dynamic light scattering, and inductively coupled plasma mass spectrometry to have a size of 16.6±2.1 nm in diameter and a TPZs/AuNPs ratio of ~700:1. In contrast with PEGylated AuNPs, the as-synthesized TPZs-AuNPs exhibited 20% increment in hydroxyl radical production in water at 2.0 Gy, and 19% increase in sensitizer enhancement ratio at 10% survival fraction for human hepatoma HepG2 cells under X-ray irradiation. The production of reactive oxygen species in HepG2 cells exposed to X-rays in vitro demonstrated a synergistic radiosensitizing effect of AuNPs and TPZ moiety. Thus, the reductive drug-conjugated TPZs-AuNPs as a kind of AuNP radiosensitizer with low gold loading provide a new strategy for enhancing the efficacy of radiation therapy. Keywords: AuNPs, radiation enhancement, synergistic effect, human hepatoma cells, hydroxyl radical production |
| format |
article |
| author |
Liu X Liu Y Zhang P Jin X Zheng X Ye F Chen W Li Q |
| author_facet |
Liu X Liu Y Zhang P Jin X Zheng X Ye F Chen W Li Q |
| author_sort |
Liu X |
| title |
The synergistic radiosensitizing effect of tirapazamine-conjugated gold nanoparticles on human hepatoma HepG2 cells under X-ray irradiation |
| title_short |
The synergistic radiosensitizing effect of tirapazamine-conjugated gold nanoparticles on human hepatoma HepG2 cells under X-ray irradiation |
| title_full |
The synergistic radiosensitizing effect of tirapazamine-conjugated gold nanoparticles on human hepatoma HepG2 cells under X-ray irradiation |
| title_fullStr |
The synergistic radiosensitizing effect of tirapazamine-conjugated gold nanoparticles on human hepatoma HepG2 cells under X-ray irradiation |
| title_full_unstemmed |
The synergistic radiosensitizing effect of tirapazamine-conjugated gold nanoparticles on human hepatoma HepG2 cells under X-ray irradiation |
| title_sort |
synergistic radiosensitizing effect of tirapazamine-conjugated gold nanoparticles on human hepatoma hepg2 cells under x-ray irradiation |
| publisher |
Dove Medical Press |
| publishDate |
2016 |
| url |
https://doaj.org/article/04e081637f274b519080812d5434a312 |
| work_keys_str_mv |
AT liux thesynergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT liuy thesynergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT zhangp thesynergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT jinx thesynergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT zhengx thesynergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT yef thesynergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT chenw thesynergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT liq thesynergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT liux synergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT liuy synergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT zhangp synergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT jinx synergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT zhengx synergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT yef synergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT chenw synergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation AT liq synergisticradiosensitizingeffectoftirapazamineconjugatedgoldnanoparticlesonhumanhepatomahepg2cellsunderxrayirradiation |
| _version_ |
1718401409803616256 |