Cholera Rapid Diagnostic Tests for the Detection of <i>Vibrio cholerae</i> O1: An Updated Meta-Analysis

The rapid diagnosis of cholera contributes to adequate outbreak management. This meta-analysis assesses the diagnostic accuracy of cholera rapid tests (RDTs) to detect <i>Vibrio cholerae</i> O1. Methods: Systematic review and meta-analysis. We searched four databases (Medline, EMBASE, Go...

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Autores principales:	Basilua Andre Muzembo, Kei Kitahara, Ayumu Ohno, Anusuya Debnath, Keinosuke Okamoto, Shin-Ichi Miyoshi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rapid test cholera <i>Vibrio cholera</i> O1 sensitivity specificity accuracy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/04e5fb2629594d17a6c99fa3133a6cc2
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spelling	oai:doaj.org-article:04e5fb2629594d17a6c99fa3133a6cc22021-11-25T17:21:35ZCholera Rapid Diagnostic Tests for the Detection of <i>Vibrio cholerae</i> O1: An Updated Meta-Analysis10.3390/diagnostics111120952075-4418https://doaj.org/article/04e5fb2629594d17a6c99fa3133a6cc22021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/2095https://doaj.org/toc/2075-4418The rapid diagnosis of cholera contributes to adequate outbreak management. This meta-analysis assesses the diagnostic accuracy of cholera rapid tests (RDTs) to detect <i>Vibrio cholerae</i> O1. Methods: Systematic review and meta-analysis. We searched four databases (Medline, EMBASE, Google Scholar, and Web of Science up to 8 September 2021) for studies that evaluated cholera RDTs for the detection of <i>V. cholerae</i> O1 compared with either stool culture or polymerase chain reaction (PCR). We assessed the studies’ quality using the QUADAS-2 criteria. In addition, in this update, GRADE approach was used to rate the overall certainty of the evidence. We performed a bivariate random-effects meta-analysis to calculate the pooled sensitivity and specificity of cholera RDTs. Results: Overall, 20 studies were included in this meta-analysis. Studies were from Africa (<i>n</i> = 11), Asia (<i>n</i> = 7), and America (Haiti; <i>n</i> = 2). They evaluated eight RDTs (Crystal VC-O1, Crystal VC, Cholkit, Institut Pasteur cholera dipstick, SD Bioline, Artron, Cholera Smart O1, and Smart II Cholera O1). Using direct specimen testing, sensitivity and specificity of RDTs were 90% (95% CI, 86 to 93) and 86% (95% CI, 81 to 90), respectively. Cholera Sensitivity was higher in studies conducted in Africa [92% (95% CI, 89 to 94)] compared with Asia [82% (95% CI, 77 to 87)]. However, specificity [83% (95% CI, 71 to 91)] was lower in Africa compared with Asia [90% (95% CI, 84 to 94)]. GRADE quality of evidence was estimated as moderate. Conclusions: Against culture or PCR, current cholera RDTs have moderate sensitivity and specificity for detecting <i>Vibrio cholerae</i> O1.Basilua Andre MuzemboKei KitaharaAyumu OhnoAnusuya DebnathKeinosuke OkamotoShin-Ichi MiyoshiMDPI AGarticlerapid testcholera<i>Vibrio cholera</i> O1sensitivityspecificityaccuracyMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 2095, p 2095 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rapid test cholera <i>Vibrio cholera</i> O1 sensitivity specificity accuracy Medicine (General) R5-920
spellingShingle	rapid test cholera <i>Vibrio cholera</i> O1 sensitivity specificity accuracy Medicine (General) R5-920 Basilua Andre Muzembo Kei Kitahara Ayumu Ohno Anusuya Debnath Keinosuke Okamoto Shin-Ichi Miyoshi Cholera Rapid Diagnostic Tests for the Detection of <i>Vibrio cholerae</i> O1: An Updated Meta-Analysis
description	The rapid diagnosis of cholera contributes to adequate outbreak management. This meta-analysis assesses the diagnostic accuracy of cholera rapid tests (RDTs) to detect <i>Vibrio cholerae</i> O1. Methods: Systematic review and meta-analysis. We searched four databases (Medline, EMBASE, Google Scholar, and Web of Science up to 8 September 2021) for studies that evaluated cholera RDTs for the detection of <i>V. cholerae</i> O1 compared with either stool culture or polymerase chain reaction (PCR). We assessed the studies’ quality using the QUADAS-2 criteria. In addition, in this update, GRADE approach was used to rate the overall certainty of the evidence. We performed a bivariate random-effects meta-analysis to calculate the pooled sensitivity and specificity of cholera RDTs. Results: Overall, 20 studies were included in this meta-analysis. Studies were from Africa (<i>n</i> = 11), Asia (<i>n</i> = 7), and America (Haiti; <i>n</i> = 2). They evaluated eight RDTs (Crystal VC-O1, Crystal VC, Cholkit, Institut Pasteur cholera dipstick, SD Bioline, Artron, Cholera Smart O1, and Smart II Cholera O1). Using direct specimen testing, sensitivity and specificity of RDTs were 90% (95% CI, 86 to 93) and 86% (95% CI, 81 to 90), respectively. Cholera Sensitivity was higher in studies conducted in Africa [92% (95% CI, 89 to 94)] compared with Asia [82% (95% CI, 77 to 87)]. However, specificity [83% (95% CI, 71 to 91)] was lower in Africa compared with Asia [90% (95% CI, 84 to 94)]. GRADE quality of evidence was estimated as moderate. Conclusions: Against culture or PCR, current cholera RDTs have moderate sensitivity and specificity for detecting <i>Vibrio cholerae</i> O1.
format	article
author	Basilua Andre Muzembo Kei Kitahara Ayumu Ohno Anusuya Debnath Keinosuke Okamoto Shin-Ichi Miyoshi
author_facet	Basilua Andre Muzembo Kei Kitahara Ayumu Ohno Anusuya Debnath Keinosuke Okamoto Shin-Ichi Miyoshi
author_sort	Basilua Andre Muzembo
title	Cholera Rapid Diagnostic Tests for the Detection of <i>Vibrio cholerae</i> O1: An Updated Meta-Analysis
title_short	Cholera Rapid Diagnostic Tests for the Detection of <i>Vibrio cholerae</i> O1: An Updated Meta-Analysis
title_full	Cholera Rapid Diagnostic Tests for the Detection of <i>Vibrio cholerae</i> O1: An Updated Meta-Analysis
title_fullStr	Cholera Rapid Diagnostic Tests for the Detection of <i>Vibrio cholerae</i> O1: An Updated Meta-Analysis
title_full_unstemmed	Cholera Rapid Diagnostic Tests for the Detection of <i>Vibrio cholerae</i> O1: An Updated Meta-Analysis
title_sort	cholera rapid diagnostic tests for the detection of <i>vibrio cholerae</i> o1: an updated meta-analysis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/04e5fb2629594d17a6c99fa3133a6cc2
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Cholera Rapid Diagnostic Tests for the Detection of <i>Vibrio cholerae</i> O1: An Updated Meta-Analysis

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