Characterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats.

Neuronal voltage-gated Ca(2+) channels are involved in electrical signalling and in converting these signals into cytoplasmic calcium changes. One important function of voltage-gated Ca(2+) channels is generating regenerative dendritic Ca(2+) spikes. However, the Ca(2+) dependent mechanisms used to...

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Autores principales:	Mara Almog, Alon Korngreen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/04ed304544e74087b361e407c220aa91
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spelling	oai:doaj.org-article:04ed304544e74087b361e407c220aa912021-11-25T06:16:24ZCharacterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats.1932-620310.1371/journal.pone.0004841https://doaj.org/article/04ed304544e74087b361e407c220aa912009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19337371/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Neuronal voltage-gated Ca(2+) channels are involved in electrical signalling and in converting these signals into cytoplasmic calcium changes. One important function of voltage-gated Ca(2+) channels is generating regenerative dendritic Ca(2+) spikes. However, the Ca(2+) dependent mechanisms used to create these spikes are only partially understood. To start investigating this mechanism, we set out to kinetically and pharmacologically identify the sub-types of somatic voltage-gated Ca(2+) channels in pyramidal neurons from layer 5 of rat somatosensory cortex, using the nucleated configuration of the patch-clamp technique. The activation kinetics of the total Ba(2+) current revealed conductance activation only at medium and high voltages suggesting that T-type calcium channels were not present in the patches. Steady-state inactivation protocols in combination with pharmacology revealed the expression of R-type channels. Furthermore, pharmacological experiments identified 5 voltage-gated Ca(2+) channel sub-types - L-, N-, R- and P/Q-type. Finally, the activation of the Ca(2+) conductances was examined using physiologically derived voltage-clamp protocols including a calcium spike protocol and a mock back-propagating action potential (mBPAP) protocol. These experiments enable us to suggest the possible contribution of the five Ca(2+) channel sub-types to Ca(2+) current flow during activation under physiological conditions.Mara AlmogAlon KorngreenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 4, p e4841 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mara Almog Alon Korngreen Characterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats.
description	Neuronal voltage-gated Ca(2+) channels are involved in electrical signalling and in converting these signals into cytoplasmic calcium changes. One important function of voltage-gated Ca(2+) channels is generating regenerative dendritic Ca(2+) spikes. However, the Ca(2+) dependent mechanisms used to create these spikes are only partially understood. To start investigating this mechanism, we set out to kinetically and pharmacologically identify the sub-types of somatic voltage-gated Ca(2+) channels in pyramidal neurons from layer 5 of rat somatosensory cortex, using the nucleated configuration of the patch-clamp technique. The activation kinetics of the total Ba(2+) current revealed conductance activation only at medium and high voltages suggesting that T-type calcium channels were not present in the patches. Steady-state inactivation protocols in combination with pharmacology revealed the expression of R-type channels. Furthermore, pharmacological experiments identified 5 voltage-gated Ca(2+) channel sub-types - L-, N-, R- and P/Q-type. Finally, the activation of the Ca(2+) conductances was examined using physiologically derived voltage-clamp protocols including a calcium spike protocol and a mock back-propagating action potential (mBPAP) protocol. These experiments enable us to suggest the possible contribution of the five Ca(2+) channel sub-types to Ca(2+) current flow during activation under physiological conditions.
format	article
author	Mara Almog Alon Korngreen
author_facet	Mara Almog Alon Korngreen
author_sort	Mara Almog
title	Characterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats.
title_short	Characterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats.
title_full	Characterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats.
title_fullStr	Characterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats.
title_full_unstemmed	Characterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats.
title_sort	characterization of voltage-gated ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/04ed304544e74087b361e407c220aa91
work_keys_str_mv	AT maraalmog characterizationofvoltagegatedca2conductancesinlayer5neocorticalpyramidalneuronsfromrats AT alonkorngreen characterizationofvoltagegatedca2conductancesinlayer5neocorticalpyramidalneuronsfromrats
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Characterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats.

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