Self-assembling peptide-based nanoparticles enhance anticancer effect of ellipticine in vitro and in vivo

Yan Wu,1,* Parisa Sadatmousavi,2,* Rong Wang,1 Sheng Lu,2 Yong-fang Yuan,1 P. Chen21Department of Pharmacy, No. 3 People&#39;s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, People&#39;s Republic of China; 2Department of Chemical Engineering, Unive...

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Autores principales:	Wu Y, Sadatmousavi P, Wang R, Lu S, Yuan YF, Chen P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/04f5861af23c4052aa7c2d04f25b8993
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spelling	oai:doaj.org-article:04f5861af23c4052aa7c2d04f25b89932021-12-02T02:52:36ZSelf-assembling peptide-based nanoparticles enhance anticancer effect of ellipticine in vitro and in vivo1176-91141178-2013https://doaj.org/article/04f5861af23c4052aa7c2d04f25b89932012-06-01T00:00:00Zhttp://www.dovepress.com/self-assembling-peptide-based-nanoparticles-enhance-anticancer-effect--a10249https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yan Wu,1,* Parisa Sadatmousavi,2,* Rong Wang,1 Sheng Lu,2 Yong-fang Yuan,1 P. Chen21Department of Pharmacy, No. 3 People&#39;s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, People&#39;s Republic of China; 2Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada *Both authors contributed equally to this work.Background and methods: Applications of the anticancer agent, ellipticine, have been limited by its hydrophobicity and toxicity. An efficient delivery system is required to exploit the enormous potential of this compound. Recently, EAK16-II, an ionic-complementary, self-assembling peptide, has been found to stabilize ellipticine in aqueous solution. Here, the anticancer activity of ellipticine encapsulated in EAK16-II (EAK-EPT) was evaluated in vitro and in vivo.Results: Our cellular uptake, toxicity, and apoptosis results in an A549 human lung carcinoma cell line indicate that EAK-EPT complexes are significantly more effective than treatment with EAK16-II or ellipticine alone. This is due to the ability of EAK16-II to stabilize ellipticine in a protonated state in well formed nanostructures approximately 200 nm in size. In vivo observations in an A549 nude mouse tumor model show higher antitumor activity and lower cytotoxicity of EAK-EPT complexes than in the control group treated with ellipticine alone. Tumor growth in animals was significantly inhibited after treatment with EAK-EPT complexes, and without any apparent side effects.Conclusion: The anticancer activity observed in this study coupled with minimal side effects encourages further development of peptide-mediated delivery of anticancer drugs, ellipticine in the present case, for clinical application.Keywords: self-assembling peptide, EAK16-II, ellipticine, nanoparticles, drug delivery, antitumorWu YSadatmousavi PWang RLu SYuan YFChen PDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3221-3233 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wu Y Sadatmousavi P Wang R Lu S Yuan YF Chen P Self-assembling peptide-based nanoparticles enhance anticancer effect of ellipticine in vitro and in vivo
description	Yan Wu,1,* Parisa Sadatmousavi,2,* Rong Wang,1 Sheng Lu,2 Yong-fang Yuan,1 P. Chen21Department of Pharmacy, No. 3 People&#39;s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, People&#39;s Republic of China; 2Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada *Both authors contributed equally to this work.Background and methods: Applications of the anticancer agent, ellipticine, have been limited by its hydrophobicity and toxicity. An efficient delivery system is required to exploit the enormous potential of this compound. Recently, EAK16-II, an ionic-complementary, self-assembling peptide, has been found to stabilize ellipticine in aqueous solution. Here, the anticancer activity of ellipticine encapsulated in EAK16-II (EAK-EPT) was evaluated in vitro and in vivo.Results: Our cellular uptake, toxicity, and apoptosis results in an A549 human lung carcinoma cell line indicate that EAK-EPT complexes are significantly more effective than treatment with EAK16-II or ellipticine alone. This is due to the ability of EAK16-II to stabilize ellipticine in a protonated state in well formed nanostructures approximately 200 nm in size. In vivo observations in an A549 nude mouse tumor model show higher antitumor activity and lower cytotoxicity of EAK-EPT complexes than in the control group treated with ellipticine alone. Tumor growth in animals was significantly inhibited after treatment with EAK-EPT complexes, and without any apparent side effects.Conclusion: The anticancer activity observed in this study coupled with minimal side effects encourages further development of peptide-mediated delivery of anticancer drugs, ellipticine in the present case, for clinical application.Keywords: self-assembling peptide, EAK16-II, ellipticine, nanoparticles, drug delivery, antitumor
format	article
author	Wu Y Sadatmousavi P Wang R Lu S Yuan YF Chen P
author_facet	Wu Y Sadatmousavi P Wang R Lu S Yuan YF Chen P
author_sort	Wu Y
title	Self-assembling peptide-based nanoparticles enhance anticancer effect of ellipticine in vitro and in vivo
title_short	Self-assembling peptide-based nanoparticles enhance anticancer effect of ellipticine in vitro and in vivo
title_full	Self-assembling peptide-based nanoparticles enhance anticancer effect of ellipticine in vitro and in vivo
title_fullStr	Self-assembling peptide-based nanoparticles enhance anticancer effect of ellipticine in vitro and in vivo
title_full_unstemmed	Self-assembling peptide-based nanoparticles enhance anticancer effect of ellipticine in vitro and in vivo
title_sort	self-assembling peptide-based nanoparticles enhance anticancer effect of ellipticine in vitro and in vivo
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/04f5861af23c4052aa7c2d04f25b8993
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Self-assembling peptide-based nanoparticles enhance anticancer effect of ellipticine in vitro and in vivo

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