Mycoplasma genitalium Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35

Mycoplasma genitalium is a newly recognized pathogen associated with sexually transmitted diseases (STDs). MgPa, the adhesion protein of Mycoplasma genitalium, is the main adhesin and the key factor for M. genitalium interacting with host cell...

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Autores principales:	Pei Dai, Xiangying Deng, Peng Liu, Lingling Li, Dan Luo, Yating Liao, Yanhua Zeng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Mycoplasma genitalium</i> MgPa T7 phage-displayed cDNA library interacting proteins RPL35 Medicine R
Acceso en línea:	https://doaj.org/article/04fced3f4cb44b2d96dc71a1a45157cc
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spelling	oai:doaj.org-article:04fced3f4cb44b2d96dc71a1a45157cc2021-11-25T18:38:21Z<i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL3510.3390/pathogens101114492076-0817https://doaj.org/article/04fced3f4cb44b2d96dc71a1a45157cc2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1449https://doaj.org/toc/2076-0817<i>Mycoplasma genitalium</i> is a newly recognized pathogen associated with sexually transmitted diseases (STDs). MgPa, the adhesion protein of <i>Mycoplasma genitalium</i>, is the main adhesin and the key factor for <i>M. genitalium</i> interacting with host cells. Currently, the long-term survival mechanism of <i>M. genitalium</i> in the host is not clear. In this study, a T7 phage-displayed human urothelial cell (SV-HUC-1) cDNA library was constructed, and the interaction of MgPa was screened from this library using the recombinant MgPa (rMgPa) as a target molecule. We verified that 60S ribosomal protein L35 (RPL35) can interact with MgPa using far-Western blot and co-localization analysis. According to the results of tandem mass tag (TMT) labeling and proteome quantitative analysis, there were altogether 407 differentially expressed proteins between the pcDNA3.1(+)/MgPa-transfected cells and non-transfected cells, of which there were 6 downregulated proteins and 401 upregulated proteins. The results of qRT-PCR demonstrated that interaction between rMgPa and RPL35 could promote the expressions of EIF2, SRP68, SERBP1, RPL35A, EGF, and TGF-β. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide bromide (MTT) assays corroborated that the interaction between rMgPa and RPL35 could promote SV-HUC-1 cell proliferation. Therefore, our findings indicated that the interaction between rMgPa and RPL35 can enhance the expressions of transcription-initiation and translation-related proteins and thus promote cell proliferation. This study elucidates a new biological function of MgPa and can explain this new mechanism of <i>M. genitalium</i> in the host.Pei DaiXiangying DengPeng LiuLingling LiDan LuoYating LiaoYanhua ZengMDPI AGarticle<i>Mycoplasma genitalium</i>MgPaT7 phage-displayed cDNA libraryinteracting proteinsRPL35MedicineRENPathogens, Vol 10, Iss 1449, p 1449 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Mycoplasma genitalium</i> MgPa T7 phage-displayed cDNA library interacting proteins RPL35 Medicine R
spellingShingle	<i>Mycoplasma genitalium</i> MgPa T7 phage-displayed cDNA library interacting proteins RPL35 Medicine R Pei Dai Xiangying Deng Peng Liu Lingling Li Dan Luo Yating Liao Yanhua Zeng <i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35
description	<i>Mycoplasma genitalium</i> is a newly recognized pathogen associated with sexually transmitted diseases (STDs). MgPa, the adhesion protein of <i>Mycoplasma genitalium</i>, is the main adhesin and the key factor for <i>M. genitalium</i> interacting with host cells. Currently, the long-term survival mechanism of <i>M. genitalium</i> in the host is not clear. In this study, a T7 phage-displayed human urothelial cell (SV-HUC-1) cDNA library was constructed, and the interaction of MgPa was screened from this library using the recombinant MgPa (rMgPa) as a target molecule. We verified that 60S ribosomal protein L35 (RPL35) can interact with MgPa using far-Western blot and co-localization analysis. According to the results of tandem mass tag (TMT) labeling and proteome quantitative analysis, there were altogether 407 differentially expressed proteins between the pcDNA3.1(+)/MgPa-transfected cells and non-transfected cells, of which there were 6 downregulated proteins and 401 upregulated proteins. The results of qRT-PCR demonstrated that interaction between rMgPa and RPL35 could promote the expressions of EIF2, SRP68, SERBP1, RPL35A, EGF, and TGF-β. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide bromide (MTT) assays corroborated that the interaction between rMgPa and RPL35 could promote SV-HUC-1 cell proliferation. Therefore, our findings indicated that the interaction between rMgPa and RPL35 can enhance the expressions of transcription-initiation and translation-related proteins and thus promote cell proliferation. This study elucidates a new biological function of MgPa and can explain this new mechanism of <i>M. genitalium</i> in the host.
format	article
author	Pei Dai Xiangying Deng Peng Liu Lingling Li Dan Luo Yating Liao Yanhua Zeng
author_facet	Pei Dai Xiangying Deng Peng Liu Lingling Li Dan Luo Yating Liao Yanhua Zeng
author_sort	Pei Dai
title	<i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35
title_short	<i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35
title_full	<i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35
title_fullStr	<i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35
title_full_unstemmed	<i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35
title_sort	<i>mycoplasma genitalium</i> protein of adhesion promotes the early proliferation of human urothelial cells by interacting with rpl35
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/04fced3f4cb44b2d96dc71a1a45157cc
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<i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35

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