Differential effects of a post-anthesis heat stress on wheat (Triticum aestivum L.) grain proteome determined by iTRAQ

Abstract Heat stress, a major abiotic stressor of wheat (Triticum aestivum L.), often results in reduced yield and decreased quality. In this study, a proteomic method, Tags for Relative and Absolute Quantitation Isobaric (iTRAQ), was adopted to analyze the protein expression profile changes among w...

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Autores principales:	Yufeng Zhang, Jiajia Pan, Xiuwen Huang, Dandan Guo, Hongyao Lou, Zhenghong Hou, Meng Su, Rongqi Liang, Chaojie Xie, Mingshan You, Baoyun Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0502dd4938aa4cb4875fb550c938ba1e
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spelling	oai:doaj.org-article:0502dd4938aa4cb4875fb550c938ba1e2021-12-02T12:32:29ZDifferential effects of a post-anthesis heat stress on wheat (Triticum aestivum L.) grain proteome determined by iTRAQ10.1038/s41598-017-03860-02045-2322https://doaj.org/article/0502dd4938aa4cb4875fb550c938ba1e2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03860-0https://doaj.org/toc/2045-2322Abstract Heat stress, a major abiotic stressor of wheat (Triticum aestivum L.), often results in reduced yield and decreased quality. In this study, a proteomic method, Tags for Relative and Absolute Quantitation Isobaric (iTRAQ), was adopted to analyze the protein expression profile changes among wheat cultivar Jing411 under heat stress. Results indicated that there were 256 different proteins expressed in Jing411 under heat stress. According to the result of gene annotation and functional classification, 239 proteins were annotated by 856 GO function entries, including growth and metabolism proteins, energy metabolism proteins, processing and storage proteins, defense-related proteins, signal transduction, unknown function proteins and hypothetical proteins. GO enrichment analysis suggested that the differentially expressed proteins in Jing411 under heat stress were mainly involved in stimulus response (67), abiotic stress response (26) and stress response (58), kinase activity (12), and transferase activity (12). Among the differentially expressed proteins in Jing411, 115 were attributed to 119 KEGG signaling/metabolic pathways. KEGG pathway enrichment analysis in Jing411 showed that heat stress mainly affected the starch and sucrose metabolism as well as protein synthesis pathway in the endoplasmic reticulum. The protein interaction network indicated that there were 8 differentially expressed proteins that could form an interaction network in Jing411.Yufeng ZhangJiajia PanXiuwen HuangDandan GuoHongyao LouZhenghong HouMeng SuRongqi LiangChaojie XieMingshan YouBaoyun LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yufeng Zhang Jiajia Pan Xiuwen Huang Dandan Guo Hongyao Lou Zhenghong Hou Meng Su Rongqi Liang Chaojie Xie Mingshan You Baoyun Li Differential effects of a post-anthesis heat stress on wheat (Triticum aestivum L.) grain proteome determined by iTRAQ
description	Abstract Heat stress, a major abiotic stressor of wheat (Triticum aestivum L.), often results in reduced yield and decreased quality. In this study, a proteomic method, Tags for Relative and Absolute Quantitation Isobaric (iTRAQ), was adopted to analyze the protein expression profile changes among wheat cultivar Jing411 under heat stress. Results indicated that there were 256 different proteins expressed in Jing411 under heat stress. According to the result of gene annotation and functional classification, 239 proteins were annotated by 856 GO function entries, including growth and metabolism proteins, energy metabolism proteins, processing and storage proteins, defense-related proteins, signal transduction, unknown function proteins and hypothetical proteins. GO enrichment analysis suggested that the differentially expressed proteins in Jing411 under heat stress were mainly involved in stimulus response (67), abiotic stress response (26) and stress response (58), kinase activity (12), and transferase activity (12). Among the differentially expressed proteins in Jing411, 115 were attributed to 119 KEGG signaling/metabolic pathways. KEGG pathway enrichment analysis in Jing411 showed that heat stress mainly affected the starch and sucrose metabolism as well as protein synthesis pathway in the endoplasmic reticulum. The protein interaction network indicated that there were 8 differentially expressed proteins that could form an interaction network in Jing411.
format	article
author	Yufeng Zhang Jiajia Pan Xiuwen Huang Dandan Guo Hongyao Lou Zhenghong Hou Meng Su Rongqi Liang Chaojie Xie Mingshan You Baoyun Li
author_facet	Yufeng Zhang Jiajia Pan Xiuwen Huang Dandan Guo Hongyao Lou Zhenghong Hou Meng Su Rongqi Liang Chaojie Xie Mingshan You Baoyun Li
author_sort	Yufeng Zhang
title	Differential effects of a post-anthesis heat stress on wheat (Triticum aestivum L.) grain proteome determined by iTRAQ
title_short	Differential effects of a post-anthesis heat stress on wheat (Triticum aestivum L.) grain proteome determined by iTRAQ
title_full	Differential effects of a post-anthesis heat stress on wheat (Triticum aestivum L.) grain proteome determined by iTRAQ
title_fullStr	Differential effects of a post-anthesis heat stress on wheat (Triticum aestivum L.) grain proteome determined by iTRAQ
title_full_unstemmed	Differential effects of a post-anthesis heat stress on wheat (Triticum aestivum L.) grain proteome determined by iTRAQ
title_sort	differential effects of a post-anthesis heat stress on wheat (triticum aestivum l.) grain proteome determined by itraq
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0502dd4938aa4cb4875fb550c938ba1e
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Differential effects of a post-anthesis heat stress on wheat (Triticum aestivum L.) grain proteome determined by iTRAQ

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