Bovine endometrium-derived cultured cells are suitable for lipofection

Abstract Bovine-derived cultured cells, including Madin-Darby bovine kidney cells, are used worldwide; however, lipofection tend to result in low transfection efficiency, which has impeded the progress of veterinary research. We performed experiments to confirm the lipofection efficiency of bovine-d...

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Autores principales:	Mai Shiokawa, Ryotaro Miura, Aki Okubo, Yujiro Hagita, Itaru Yoshimura, Hiroshi Aoki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/050511aca71741dfb300e90a99e3582a
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spelling	oai:doaj.org-article:050511aca71741dfb300e90a99e3582a2021-12-02T15:07:54ZBovine endometrium-derived cultured cells are suitable for lipofection10.1038/s41598-021-95848-02045-2322https://doaj.org/article/050511aca71741dfb300e90a99e3582a2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95848-0https://doaj.org/toc/2045-2322Abstract Bovine-derived cultured cells, including Madin-Darby bovine kidney cells, are used worldwide; however, lipofection tend to result in low transfection efficiency, which has impeded the progress of veterinary research. We performed experiments to confirm the lipofection efficiency of bovine-derived cultured cells, to identify cells that suitable for lipofection. Several bovine tissues (endometrium, testis, ear tissue and foetal muscle) were collected, and primary cultured cells were prepared. Lipofection assay showed that only bovine endometrium (BE)-derived cells could be transfected efficiently (50‒70%). BE cells can be divided into at least two types of cell populations (BE-1 and BE-2). The BE-1 cells, which were suitable for lipofection, were obtained by passages at short intervals and were negative for cytokeratin- and positive for vimentin-expression; the BE-2 cells did not have these characteristics and were not suitable for lipofection. Furthermore, the BE-1 cells and artificially immortalised cells of BE-1, iBE-1 cells, were utilised in a reporter assay requiring the introduction of multiple DNAs. Endometrial tissues can be collected from living cows, and BE-1 cells can be obtained easily by controlling passaging timing. The production of BE-1 cells and sharing the methods required to prepare them will contribute to the development of veterinary research.Mai ShiokawaRyotaro MiuraAki OkuboYujiro HagitaItaru YoshimuraHiroshi AokiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mai Shiokawa Ryotaro Miura Aki Okubo Yujiro Hagita Itaru Yoshimura Hiroshi Aoki Bovine endometrium-derived cultured cells are suitable for lipofection
description	Abstract Bovine-derived cultured cells, including Madin-Darby bovine kidney cells, are used worldwide; however, lipofection tend to result in low transfection efficiency, which has impeded the progress of veterinary research. We performed experiments to confirm the lipofection efficiency of bovine-derived cultured cells, to identify cells that suitable for lipofection. Several bovine tissues (endometrium, testis, ear tissue and foetal muscle) were collected, and primary cultured cells were prepared. Lipofection assay showed that only bovine endometrium (BE)-derived cells could be transfected efficiently (50‒70%). BE cells can be divided into at least two types of cell populations (BE-1 and BE-2). The BE-1 cells, which were suitable for lipofection, were obtained by passages at short intervals and were negative for cytokeratin- and positive for vimentin-expression; the BE-2 cells did not have these characteristics and were not suitable for lipofection. Furthermore, the BE-1 cells and artificially immortalised cells of BE-1, iBE-1 cells, were utilised in a reporter assay requiring the introduction of multiple DNAs. Endometrial tissues can be collected from living cows, and BE-1 cells can be obtained easily by controlling passaging timing. The production of BE-1 cells and sharing the methods required to prepare them will contribute to the development of veterinary research.
format	article
author	Mai Shiokawa Ryotaro Miura Aki Okubo Yujiro Hagita Itaru Yoshimura Hiroshi Aoki
author_facet	Mai Shiokawa Ryotaro Miura Aki Okubo Yujiro Hagita Itaru Yoshimura Hiroshi Aoki
author_sort	Mai Shiokawa
title	Bovine endometrium-derived cultured cells are suitable for lipofection
title_short	Bovine endometrium-derived cultured cells are suitable for lipofection
title_full	Bovine endometrium-derived cultured cells are suitable for lipofection
title_fullStr	Bovine endometrium-derived cultured cells are suitable for lipofection
title_full_unstemmed	Bovine endometrium-derived cultured cells are suitable for lipofection
title_sort	bovine endometrium-derived cultured cells are suitable for lipofection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/050511aca71741dfb300e90a99e3582a
work_keys_str_mv	AT maishiokawa bovineendometriumderivedculturedcellsaresuitableforlipofection AT ryotaromiura bovineendometriumderivedculturedcellsaresuitableforlipofection AT akiokubo bovineendometriumderivedculturedcellsaresuitableforlipofection AT yujirohagita bovineendometriumderivedculturedcellsaresuitableforlipofection AT itaruyoshimura bovineendometriumderivedculturedcellsaresuitableforlipofection AT hiroshiaoki bovineendometriumderivedculturedcellsaresuitableforlipofection
_version_	1718388298083205120

Bovine endometrium-derived cultured cells are suitable for lipofection

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