Development of an advanced flow cytometry based high-resolution immunophenotyping method to benchmark early immune response in dairy cows

Abstract The determination of the somatic cell count of a milk sample is one of the most common methods to monitor udder health of a dairy cow. However, this procedure does not take into account the fact that cells in milk present a great variety of different cell types. The objective of our study w...

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Autores principales:	Sabine Farschtschi, Martin Mattes, Alex Hildebrandt, Dapi Chiang, Benedikt Kirchner, Heike Kliem, Michael W. Pfaffl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/050adbb763bb41d18f44cbc771224d32
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spelling	oai:doaj.org-article:050adbb763bb41d18f44cbc771224d322021-11-28T12:18:15ZDevelopment of an advanced flow cytometry based high-resolution immunophenotyping method to benchmark early immune response in dairy cows10.1038/s41598-021-02405-w2045-2322https://doaj.org/article/050adbb763bb41d18f44cbc771224d322021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02405-whttps://doaj.org/toc/2045-2322Abstract The determination of the somatic cell count of a milk sample is one of the most common methods to monitor udder health of a dairy cow. However, this procedure does not take into account the fact that cells in milk present a great variety of different cell types. The objective of our study was to establish a high-resolution differential cell count (HRDCC) by means of flow cytometry in blood and milk. We were able to detect ten subpopulations among the three main populations of immune cells and to determine their viability. Additionally, blood samples were analyzed for common laboratory biomarkers, i.e. differential blood counts, haptoglobin levels and several metabolic parameters. In this first feasibility study, we used three different vaccines to stimulate the immune system of five healthy cows each. Samples were collected shortly before, in between and after the vaccinations. Using multivariate statistical methods we saw a diagnostic benefit when HRDCCs were included compared to only the standard laboratory parameters. The impacts of all three vaccinations on the immune system were visible in blood HRDCCs as well as in milk HRDCCs. Cluster of Differentiation 8+ (CD8+) T cells, B cells and monocyte/macrophage subpopulations were among the most important and statistically relevant parameters for all treatments in both biofluids. Moreover, in one of the treatment groups intermediate monocytes showed a significant increase after both vaccinations. Although the use of HRDCC in blood or milk was shown to be highly relevant for early systemic diagnostic, to confirm these subpopulations further investigations in cows of different breed, lactation stage or health status are required.Sabine FarschtschiMartin MattesAlex HildebrandtDapi ChiangBenedikt KirchnerHeike KliemMichael W. PfafflNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sabine Farschtschi Martin Mattes Alex Hildebrandt Dapi Chiang Benedikt Kirchner Heike Kliem Michael W. Pfaffl Development of an advanced flow cytometry based high-resolution immunophenotyping method to benchmark early immune response in dairy cows
description	Abstract The determination of the somatic cell count of a milk sample is one of the most common methods to monitor udder health of a dairy cow. However, this procedure does not take into account the fact that cells in milk present a great variety of different cell types. The objective of our study was to establish a high-resolution differential cell count (HRDCC) by means of flow cytometry in blood and milk. We were able to detect ten subpopulations among the three main populations of immune cells and to determine their viability. Additionally, blood samples were analyzed for common laboratory biomarkers, i.e. differential blood counts, haptoglobin levels and several metabolic parameters. In this first feasibility study, we used three different vaccines to stimulate the immune system of five healthy cows each. Samples were collected shortly before, in between and after the vaccinations. Using multivariate statistical methods we saw a diagnostic benefit when HRDCCs were included compared to only the standard laboratory parameters. The impacts of all three vaccinations on the immune system were visible in blood HRDCCs as well as in milk HRDCCs. Cluster of Differentiation 8+ (CD8+) T cells, B cells and monocyte/macrophage subpopulations were among the most important and statistically relevant parameters for all treatments in both biofluids. Moreover, in one of the treatment groups intermediate monocytes showed a significant increase after both vaccinations. Although the use of HRDCC in blood or milk was shown to be highly relevant for early systemic diagnostic, to confirm these subpopulations further investigations in cows of different breed, lactation stage or health status are required.
format	article
author	Sabine Farschtschi Martin Mattes Alex Hildebrandt Dapi Chiang Benedikt Kirchner Heike Kliem Michael W. Pfaffl
author_facet	Sabine Farschtschi Martin Mattes Alex Hildebrandt Dapi Chiang Benedikt Kirchner Heike Kliem Michael W. Pfaffl
author_sort	Sabine Farschtschi
title	Development of an advanced flow cytometry based high-resolution immunophenotyping method to benchmark early immune response in dairy cows
title_short	Development of an advanced flow cytometry based high-resolution immunophenotyping method to benchmark early immune response in dairy cows
title_full	Development of an advanced flow cytometry based high-resolution immunophenotyping method to benchmark early immune response in dairy cows
title_fullStr	Development of an advanced flow cytometry based high-resolution immunophenotyping method to benchmark early immune response in dairy cows
title_full_unstemmed	Development of an advanced flow cytometry based high-resolution immunophenotyping method to benchmark early immune response in dairy cows
title_sort	development of an advanced flow cytometry based high-resolution immunophenotyping method to benchmark early immune response in dairy cows
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/050adbb763bb41d18f44cbc771224d32
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Development of an advanced flow cytometry based high-resolution immunophenotyping method to benchmark early immune response in dairy cows

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