Two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila.

SF3B1 mutations occur in many cancers, and the highly conserved His662 residue is one of the hotspot mutation sites. To address effects on splicing and development, we constructed strains carrying point mutations at the corresponding residue His698 in Drosophila using the CRISPR-Cas9 technique. Two...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bei Zhang, Zhan Ding, Liang Li, Ling-Kun Xie, Yu-Jie Fan, Yong-Zhen Xu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/0512759a030b4e44916a5b22d013a568
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0512759a030b4e44916a5b22d013a568
record_format	dspace
spelling	oai:doaj.org-article:0512759a030b4e44916a5b22d013a5682021-12-02T20:03:17ZTwo oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila.1553-73901553-740410.1371/journal.pgen.1009861https://doaj.org/article/0512759a030b4e44916a5b22d013a5682021-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009861https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404SF3B1 mutations occur in many cancers, and the highly conserved His662 residue is one of the hotspot mutation sites. To address effects on splicing and development, we constructed strains carrying point mutations at the corresponding residue His698 in Drosophila using the CRISPR-Cas9 technique. Two mutations, H698D and H698R, were selected due to their frequent presence in patients and notable opposite charges. Both the sf3b1-H698D and-H698R mutant flies exhibit developmental defects, including less egg-laying, decreased hatching rates, delayed morphogenesis and shorter lifespans. Interestingly, the H698D mutant has decreased resistance to fungal infection, while the H698R mutant shows impaired climbing ability. Consistent with these phenotypes, further analysis of RNA-seq data finds altered expression of immune response genes and changed alternative splicing of muscle and neural-related genes in the two mutants, respectively. Expression of Mef2-RB, an isoform of Mef2 gene that was downregulated due to splicing changes caused by H698R, partly rescues the climbing defects of the sf3b1-H698R mutant. Lariat sequencing reveals that the two sf3b1-H698 mutations cause aberrant selection of multiple intronic branch sites, with the H698R mutant using far upstream branch sites in the changed alternative splicing events. This study provides in vivo evidence from Drosophila that elucidates how these SF3B1 hotspot mutations alter splicing and their consequences in development and in the immune system.Bei ZhangZhan DingLiang LiLing-Kun XieYu-Jie FanYong-Zhen XuPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 11, p e1009861 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Bei Zhang Zhan Ding Liang Li Ling-Kun Xie Yu-Jie Fan Yong-Zhen Xu Two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila.
description	SF3B1 mutations occur in many cancers, and the highly conserved His662 residue is one of the hotspot mutation sites. To address effects on splicing and development, we constructed strains carrying point mutations at the corresponding residue His698 in Drosophila using the CRISPR-Cas9 technique. Two mutations, H698D and H698R, were selected due to their frequent presence in patients and notable opposite charges. Both the sf3b1-H698D and-H698R mutant flies exhibit developmental defects, including less egg-laying, decreased hatching rates, delayed morphogenesis and shorter lifespans. Interestingly, the H698D mutant has decreased resistance to fungal infection, while the H698R mutant shows impaired climbing ability. Consistent with these phenotypes, further analysis of RNA-seq data finds altered expression of immune response genes and changed alternative splicing of muscle and neural-related genes in the two mutants, respectively. Expression of Mef2-RB, an isoform of Mef2 gene that was downregulated due to splicing changes caused by H698R, partly rescues the climbing defects of the sf3b1-H698R mutant. Lariat sequencing reveals that the two sf3b1-H698 mutations cause aberrant selection of multiple intronic branch sites, with the H698R mutant using far upstream branch sites in the changed alternative splicing events. This study provides in vivo evidence from Drosophila that elucidates how these SF3B1 hotspot mutations alter splicing and their consequences in development and in the immune system.
format	article
author	Bei Zhang Zhan Ding Liang Li Ling-Kun Xie Yu-Jie Fan Yong-Zhen Xu
author_facet	Bei Zhang Zhan Ding Liang Li Ling-Kun Xie Yu-Jie Fan Yong-Zhen Xu
author_sort	Bei Zhang
title	Two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila.
title_short	Two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila.
title_full	Two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila.
title_fullStr	Two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila.
title_full_unstemmed	Two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila.
title_sort	two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in drosophila.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0512759a030b4e44916a5b22d013a568
work_keys_str_mv	AT beizhang twooppositelychargedsf3b1mutationscausedefectivedevelopmentimpairedimmuneresponseandaberrantselectionofintronicbranchsitesindrosophila AT zhanding twooppositelychargedsf3b1mutationscausedefectivedevelopmentimpairedimmuneresponseandaberrantselectionofintronicbranchsitesindrosophila AT liangli twooppositelychargedsf3b1mutationscausedefectivedevelopmentimpairedimmuneresponseandaberrantselectionofintronicbranchsitesindrosophila AT lingkunxie twooppositelychargedsf3b1mutationscausedefectivedevelopmentimpairedimmuneresponseandaberrantselectionofintronicbranchsitesindrosophila AT yujiefan twooppositelychargedsf3b1mutationscausedefectivedevelopmentimpairedimmuneresponseandaberrantselectionofintronicbranchsitesindrosophila AT yongzhenxu twooppositelychargedsf3b1mutationscausedefectivedevelopmentimpairedimmuneresponseandaberrantselectionofintronicbranchsitesindrosophila
_version_	1718375642458750976

Two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila.

Ejemplares similares