Effect of Shear Stress on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Isolated from the Cystic Fibrosis Lung

ABSTRACT Chronic colonization of the lungs by Pseudomonas aeruginosa is one of the major causes of morbidity and mortality in cystic fibrosis (CF) patients. To gain insights into the characteristic biofilm phenotype of P. aeruginosa in the CF lungs, mimicking the CF lung environment is critical. We...

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Autores principales:	Jozef Dingemans, Pieter Monsieurs, Sung-Huan Yu, Aurélie Crabbé, Konrad U. Förstner, Anne Malfroot, Pierre Cornelis, Rob Van Houdt
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/05128c73b386434ea76ccf56e7e1fe95
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spelling	oai:doaj.org-article:05128c73b386434ea76ccf56e7e1fe952021-11-15T15:50:18ZEffect of Shear Stress on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Isolated from the Cystic Fibrosis Lung10.1128/mBio.00813-162150-7511https://doaj.org/article/05128c73b386434ea76ccf56e7e1fe952016-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00813-16https://doaj.org/toc/2150-7511ABSTRACT Chronic colonization of the lungs by Pseudomonas aeruginosa is one of the major causes of morbidity and mortality in cystic fibrosis (CF) patients. To gain insights into the characteristic biofilm phenotype of P. aeruginosa in the CF lungs, mimicking the CF lung environment is critical. We previously showed that growth of the non-CF-adapted P. aeruginosa PAO1 strain in a rotating wall vessel, a device that simulates the low fluid shear (LS) conditions present in the CF lung, leads to the formation of in-suspension, self-aggregating biofilms. In the present study, we determined the phenotypic and transcriptomic changes associated with the growth of a highly adapted, transmissible P. aeruginosa CF strain in artificial sputum medium under LS conditions. Robust self-aggregating biofilms were observed only under LS conditions. Growth under LS conditions resulted in the upregulation of genes involved in stress response, alginate biosynthesis, denitrification, glycine betaine biosynthesis, glycerol metabolism, and cell shape maintenance, while genes involved in phenazine biosynthesis, type VI secretion, and multidrug efflux were downregulated. In addition, a number of small RNAs appeared to be involved in the response to shear stress. Finally, quorum sensing was found to be slightly but significantly affected by shear stress, resulting in higher production of autoinducer molecules during growth under high fluid shear (HS) conditions. In summary, our study revealed a way to modulate the behavior of a highly adapted P. aeruginosa CF strain by means of introducing shear stress, driving it from a biofilm lifestyle to a more planktonic lifestyle. IMPORTANCE Biofilm formation by Pseudomonas aeruginosa is one of the hallmarks of chronic cystic fibrosis (CF) lung infections. The biofilm matrix protects this bacterium from antibiotics as well as from the immune system. Hence, the prevention or reversion of biofilm formation is believed to have a great impact on treatment of chronic P. aeruginosa CF lung infections. In the present study, we showed that it is possible to modulate the behavior of a highly adapted transmissible P. aeruginosa CF isolate at both the transcriptomic and phenotypic levels by introducing shear stress in a CF-like environment, driving it from a biofilm to a planktonic lifestyle. Consequently, the results obtained in this study are of great importance with regard to therapeutic applications that introduce shear stress in the lungs of CF patients.Jozef DingemansPieter MonsieursSung-Huan YuAurélie CrabbéKonrad U. FörstnerAnne MalfrootPierre CornelisRob Van HoudtAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 4 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Jozef Dingemans Pieter Monsieurs Sung-Huan Yu Aurélie Crabbé Konrad U. Förstner Anne Malfroot Pierre Cornelis Rob Van Houdt Effect of Shear Stress on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Isolated from the Cystic Fibrosis Lung
description	ABSTRACT Chronic colonization of the lungs by Pseudomonas aeruginosa is one of the major causes of morbidity and mortality in cystic fibrosis (CF) patients. To gain insights into the characteristic biofilm phenotype of P. aeruginosa in the CF lungs, mimicking the CF lung environment is critical. We previously showed that growth of the non-CF-adapted P. aeruginosa PAO1 strain in a rotating wall vessel, a device that simulates the low fluid shear (LS) conditions present in the CF lung, leads to the formation of in-suspension, self-aggregating biofilms. In the present study, we determined the phenotypic and transcriptomic changes associated with the growth of a highly adapted, transmissible P. aeruginosa CF strain in artificial sputum medium under LS conditions. Robust self-aggregating biofilms were observed only under LS conditions. Growth under LS conditions resulted in the upregulation of genes involved in stress response, alginate biosynthesis, denitrification, glycine betaine biosynthesis, glycerol metabolism, and cell shape maintenance, while genes involved in phenazine biosynthesis, type VI secretion, and multidrug efflux were downregulated. In addition, a number of small RNAs appeared to be involved in the response to shear stress. Finally, quorum sensing was found to be slightly but significantly affected by shear stress, resulting in higher production of autoinducer molecules during growth under high fluid shear (HS) conditions. In summary, our study revealed a way to modulate the behavior of a highly adapted P. aeruginosa CF strain by means of introducing shear stress, driving it from a biofilm lifestyle to a more planktonic lifestyle. IMPORTANCE Biofilm formation by Pseudomonas aeruginosa is one of the hallmarks of chronic cystic fibrosis (CF) lung infections. The biofilm matrix protects this bacterium from antibiotics as well as from the immune system. Hence, the prevention or reversion of biofilm formation is believed to have a great impact on treatment of chronic P. aeruginosa CF lung infections. In the present study, we showed that it is possible to modulate the behavior of a highly adapted transmissible P. aeruginosa CF isolate at both the transcriptomic and phenotypic levels by introducing shear stress in a CF-like environment, driving it from a biofilm to a planktonic lifestyle. Consequently, the results obtained in this study are of great importance with regard to therapeutic applications that introduce shear stress in the lungs of CF patients.
format	article
author	Jozef Dingemans Pieter Monsieurs Sung-Huan Yu Aurélie Crabbé Konrad U. Förstner Anne Malfroot Pierre Cornelis Rob Van Houdt
author_facet	Jozef Dingemans Pieter Monsieurs Sung-Huan Yu Aurélie Crabbé Konrad U. Förstner Anne Malfroot Pierre Cornelis Rob Van Houdt
author_sort	Jozef Dingemans
title	Effect of Shear Stress on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Isolated from the Cystic Fibrosis Lung
title_short	Effect of Shear Stress on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Isolated from the Cystic Fibrosis Lung
title_full	Effect of Shear Stress on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Isolated from the Cystic Fibrosis Lung
title_fullStr	Effect of Shear Stress on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Isolated from the Cystic Fibrosis Lung
title_full_unstemmed	Effect of Shear Stress on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Isolated from the Cystic Fibrosis Lung
title_sort	effect of shear stress on <named-content content-type="genus-species">pseudomonas aeruginosa</named-content> isolated from the cystic fibrosis lung
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/05128c73b386434ea76ccf56e7e1fe95
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Effect of Shear Stress on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Isolated from the Cystic Fibrosis Lung

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