Risks of antibiotic resistance genes and antimicrobial resistance under chlorination disinfection with public health concerns

As a widely used disinfection technology, the effects of chlorination on antibiotic resistome and bacterial community received great scientific concerns, while the pathogens associated health risks kept largely unknown. With this concern, the present study used metagenomic analysis combined with cul...

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Autores principales:	Liping Ma, Huiying Yang, Lei Guan, Xiaoyu Liu, Tong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Chlorination disinfection Escherichia coli Antibiotic resistance genes Antibiotic susceptibility Bacterial host Metagenomic binning Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/05165e551b5844d880ec74dd56ffd8fc
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spelling	oai:doaj.org-article:05165e551b5844d880ec74dd56ffd8fc2021-11-14T04:28:28ZRisks of antibiotic resistance genes and antimicrobial resistance under chlorination disinfection with public health concerns0160-412010.1016/j.envint.2021.106978https://doaj.org/article/05165e551b5844d880ec74dd56ffd8fc2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0160412021006036https://doaj.org/toc/0160-4120As a widely used disinfection technology, the effects of chlorination on antibiotic resistome and bacterial community received great scientific concerns, while the pathogens associated health risks kept largely unknown. With this concern, the present study used metagenomic analysis combined with culture method to reveal chlorination effects on antibiotic resistance genes (ARGs) and their bacterial hosts (total microbes and Escherichia coli) through simulating the chlorination dosage with human health concerns (drinking water and swimming pool). The resistome profiling showed that chlorination process could significantly decrease both abundance and diversity of total ARGs, while with limited removal rates of 6.0–8.7% for opportunistic pathogens E. coli isolates. Of all the observed 515 ARG subtypes, 105 core subtypes were identified and persistent during chlorination for both total microbes and E. coli. Antibiotic susceptibility test showed that chlorination treatment could efficiently remove multi-resistant E. coli isolates but select for tetracycline resistant isolates. Five ARG-carrying genomes (assigned to Bacteroidetes, Firmicutes, Actinobacteria) enriched by 18.1–102% after chlorination were retrieved by using metagenomic binning strategies. Bray-Curtis dissimilarity, network and procrustes analyses all indicated the remained antibiotic resistome and bacterial community were mainly chlorination-driven. Furthermore, a systematic pipeline for monitoring chlorination-associated antimicrobial resistance risks was proposed. These together enhance our knowledge of chlorination treatment associated public concerns, as important reference and guidance for surveillance and control of antibiotic resistance.Liping MaHuiying YangLei GuanXiaoyu LiuTong ZhangElsevierarticleChlorination disinfectionEscherichia coliAntibiotic resistance genesAntibiotic susceptibilityBacterial hostMetagenomic binningEnvironmental sciencesGE1-350ENEnvironment International, Vol 158, Iss , Pp 106978- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chlorination disinfection Escherichia coli Antibiotic resistance genes Antibiotic susceptibility Bacterial host Metagenomic binning Environmental sciences GE1-350
spellingShingle	Chlorination disinfection Escherichia coli Antibiotic resistance genes Antibiotic susceptibility Bacterial host Metagenomic binning Environmental sciences GE1-350 Liping Ma Huiying Yang Lei Guan Xiaoyu Liu Tong Zhang Risks of antibiotic resistance genes and antimicrobial resistance under chlorination disinfection with public health concerns
description	As a widely used disinfection technology, the effects of chlorination on antibiotic resistome and bacterial community received great scientific concerns, while the pathogens associated health risks kept largely unknown. With this concern, the present study used metagenomic analysis combined with culture method to reveal chlorination effects on antibiotic resistance genes (ARGs) and their bacterial hosts (total microbes and Escherichia coli) through simulating the chlorination dosage with human health concerns (drinking water and swimming pool). The resistome profiling showed that chlorination process could significantly decrease both abundance and diversity of total ARGs, while with limited removal rates of 6.0–8.7% for opportunistic pathogens E. coli isolates. Of all the observed 515 ARG subtypes, 105 core subtypes were identified and persistent during chlorination for both total microbes and E. coli. Antibiotic susceptibility test showed that chlorination treatment could efficiently remove multi-resistant E. coli isolates but select for tetracycline resistant isolates. Five ARG-carrying genomes (assigned to Bacteroidetes, Firmicutes, Actinobacteria) enriched by 18.1–102% after chlorination were retrieved by using metagenomic binning strategies. Bray-Curtis dissimilarity, network and procrustes analyses all indicated the remained antibiotic resistome and bacterial community were mainly chlorination-driven. Furthermore, a systematic pipeline for monitoring chlorination-associated antimicrobial resistance risks was proposed. These together enhance our knowledge of chlorination treatment associated public concerns, as important reference and guidance for surveillance and control of antibiotic resistance.
format	article
author	Liping Ma Huiying Yang Lei Guan Xiaoyu Liu Tong Zhang
author_facet	Liping Ma Huiying Yang Lei Guan Xiaoyu Liu Tong Zhang
author_sort	Liping Ma
title	Risks of antibiotic resistance genes and antimicrobial resistance under chlorination disinfection with public health concerns
title_short	Risks of antibiotic resistance genes and antimicrobial resistance under chlorination disinfection with public health concerns
title_full	Risks of antibiotic resistance genes and antimicrobial resistance under chlorination disinfection with public health concerns
title_fullStr	Risks of antibiotic resistance genes and antimicrobial resistance under chlorination disinfection with public health concerns
title_full_unstemmed	Risks of antibiotic resistance genes and antimicrobial resistance under chlorination disinfection with public health concerns
title_sort	risks of antibiotic resistance genes and antimicrobial resistance under chlorination disinfection with public health concerns
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/05165e551b5844d880ec74dd56ffd8fc
work_keys_str_mv	AT lipingma risksofantibioticresistancegenesandantimicrobialresistanceunderchlorinationdisinfectionwithpublichealthconcerns AT huiyingyang risksofantibioticresistancegenesandantimicrobialresistanceunderchlorinationdisinfectionwithpublichealthconcerns AT leiguan risksofantibioticresistancegenesandantimicrobialresistanceunderchlorinationdisinfectionwithpublichealthconcerns AT xiaoyuliu risksofantibioticresistancegenesandantimicrobialresistanceunderchlorinationdisinfectionwithpublichealthconcerns AT tongzhang risksofantibioticresistancegenesandantimicrobialresistanceunderchlorinationdisinfectionwithpublichealthconcerns
_version_	1718429998152417280

Risks of antibiotic resistance genes and antimicrobial resistance under chlorination disinfection with public health concerns

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