Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode

Guangxian Zhong,1,2,* Ruilong Lan,3,* Wenxin Zhang,1,4 Feihuan Fu,5 Yiming Sun,1,4 Huaping Peng,1,4 Tianbin Chen,3 Yishan Cai,6 Ailin Liu,1,4 Jianhua Lin,2 Xinhua Lin1,4 1Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, 2Department of Orthopaedics, 3The Centra...

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Autores principales:	Zhong G, Lan R, Zhang W, Fu F, Sun Y, Peng H, Chen T, Cai Y, Liu A, Lin J, Lin X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0518559bdb924915a783eb2906f7ac84
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spelling	oai:doaj.org-article:0518559bdb924915a783eb2906f7ac842021-12-02T05:38:25ZSensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode1178-2013https://doaj.org/article/0518559bdb924915a783eb2906f7ac842015-03-01T00:00:00Zhttp://www.dovepress.com/sensitive-electrochemical-immunosensor-based-on-three-dimensional-nano-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Guangxian Zhong,1,2,* Ruilong Lan,3,* Wenxin Zhang,1,4 Feihuan Fu,5 Yiming Sun,1,4 Huaping Peng,1,4 Tianbin Chen,3 Yishan Cai,6 Ailin Liu,1,4 Jianhua Lin,2 Xinhua Lin1,4 1Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, 2Department of Orthopaedics, 3The Centralab, First Affiliated Hospital of Fujian Medical University, 4Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, 5Department of Endocrinology, The County Hospital of Anxi, Anxi, 6Fujian International Travel Healthcare Center, Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou, People’s Republic of China *These authors contributed equally to this work Abstract: A sensitive electrochemical immunosensor was developed for detection of alpha-fetoprotein (AFP) based on a three-dimensional nanostructure gold electrode using a facile, rapid, “green” square-wave oxidation-reduction cycle technique. The resulting three-dimensional gold nanocomposites were characterized by scanning electron microscopy and cyclic voltammetry. A “sandwich-type” detection strategy using an electrochemical immunosensor was employed. Under optimal conditions, a good linear relationship between the current response signal and the AFP concentrations was observed in the range of 10–50 ng/mL with a detection limit of 3 pg/mL. This new immunosensor showed a fast amperometric response and high sensitivity and selectivity. It was successfully used to determine AFP in a human serum sample with a relative standard deviation of <5% (n=5). The proposed immunosensor represents a significant step toward practical application in clinical diagnosis and monitoring of prognosis. Keywords: electrochemical immunosensors, three-dimensional nanostructure gold electrode, square-wave oxidation-reduction cycle, alpha-fetoprotein Zhong GLan RZhang WFu FSun YPeng HChen TCai YLiu ALin JLin XDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 2219-2228 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhong G Lan R Zhang W Fu F Sun Y Peng H Chen T Cai Y Liu A Lin J Lin X Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode
description	Guangxian Zhong,1,2,* Ruilong Lan,3,* Wenxin Zhang,1,4 Feihuan Fu,5 Yiming Sun,1,4 Huaping Peng,1,4 Tianbin Chen,3 Yishan Cai,6 Ailin Liu,1,4 Jianhua Lin,2 Xinhua Lin1,4 1Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, 2Department of Orthopaedics, 3The Centralab, First Affiliated Hospital of Fujian Medical University, 4Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, 5Department of Endocrinology, The County Hospital of Anxi, Anxi, 6Fujian International Travel Healthcare Center, Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou, People’s Republic of China *These authors contributed equally to this work Abstract: A sensitive electrochemical immunosensor was developed for detection of alpha-fetoprotein (AFP) based on a three-dimensional nanostructure gold electrode using a facile, rapid, “green” square-wave oxidation-reduction cycle technique. The resulting three-dimensional gold nanocomposites were characterized by scanning electron microscopy and cyclic voltammetry. A “sandwich-type” detection strategy using an electrochemical immunosensor was employed. Under optimal conditions, a good linear relationship between the current response signal and the AFP concentrations was observed in the range of 10–50 ng/mL with a detection limit of 3 pg/mL. This new immunosensor showed a fast amperometric response and high sensitivity and selectivity. It was successfully used to determine AFP in a human serum sample with a relative standard deviation of <5% (n=5). The proposed immunosensor represents a significant step toward practical application in clinical diagnosis and monitoring of prognosis. Keywords: electrochemical immunosensors, three-dimensional nanostructure gold electrode, square-wave oxidation-reduction cycle, alpha-fetoprotein
format	article
author	Zhong G Lan R Zhang W Fu F Sun Y Peng H Chen T Cai Y Liu A Lin J Lin X
author_facet	Zhong G Lan R Zhang W Fu F Sun Y Peng H Chen T Cai Y Liu A Lin J Lin X
author_sort	Zhong G
title	Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode
title_short	Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode
title_full	Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode
title_fullStr	Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode
title_full_unstemmed	Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode
title_sort	sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/0518559bdb924915a783eb2906f7ac84
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_version_	1718400273275158528

Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode

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