Effect of NSAIDs Supplementation on the PACAP-, SP- and GAL-Immunoreactive Neurons in the Porcine Jejunum

Side effects associated with nonsteroidal anti-inflammatory drugs (NSAIDs) treatment are a serious limitation of their use in anti-inflammatory therapy. The negative effects of taking NSAIDs include abdominal pain, indigestion nausea as well as serious complications such as bleeding and perforation....

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Autores principales:	Marta Brzozowska, Barbara Jana, Jarosław Całka
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	NSAIDs ENS peripheral nervous system jejunum pig Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/051ace0b69314c86bccf896030f3df6a
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spelling	oai:doaj.org-article:051ace0b69314c86bccf896030f3df6a2021-11-11T17:09:10ZEffect of NSAIDs Supplementation on the PACAP-, SP- and GAL-Immunoreactive Neurons in the Porcine Jejunum10.3390/ijms2221116891422-00671661-6596https://doaj.org/article/051ace0b69314c86bccf896030f3df6a2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11689https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Side effects associated with nonsteroidal anti-inflammatory drugs (NSAIDs) treatment are a serious limitation of their use in anti-inflammatory therapy. The negative effects of taking NSAIDs include abdominal pain, indigestion nausea as well as serious complications such as bleeding and perforation. The enteric nervous system is involved in regulation of gastrointestinal functions through the release of neurotransmitters. The present study was designed to determine, for the first time, the changes in pituitary adenylate cyclase-activating polypeptide (PACAP), substance P (SP) and galanin (GAL) expression in porcine jejunum after long-term treatment with aspirin, indomethacin and naproxen. The study was performed on 16 immature pigs. The animals were randomly divided into four experimental groups: control, aspirin, indomethacin and naproxen. Control animals were given empty gelatin capsules, while animals in the test groups received selected NSAIDs for 28 days. Next, animals from each group were euthanized. Frozen sections were prepared from collected jejunum and subjected to double immunofluorescence staining. NSAIDs supplementation caused a significant increase in the population of PACAP-, SP- and GAL-containing enteric neurons in the porcine jejunum. Our results suggest the participation of the selected neurotransmitters in regulatory processes of the gastrointestinal function and may indicate the direct toxic effect of NSAIDs on the ENS neurons.Marta BrzozowskaBarbara JanaJarosław CałkaMDPI AGarticleNSAIDsENSperipheral nervous systemjejunumpigBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11689, p 11689 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	NSAIDs ENS peripheral nervous system jejunum pig Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	NSAIDs ENS peripheral nervous system jejunum pig Biology (General) QH301-705.5 Chemistry QD1-999 Marta Brzozowska Barbara Jana Jarosław Całka Effect of NSAIDs Supplementation on the PACAP-, SP- and GAL-Immunoreactive Neurons in the Porcine Jejunum
description	Side effects associated with nonsteroidal anti-inflammatory drugs (NSAIDs) treatment are a serious limitation of their use in anti-inflammatory therapy. The negative effects of taking NSAIDs include abdominal pain, indigestion nausea as well as serious complications such as bleeding and perforation. The enteric nervous system is involved in regulation of gastrointestinal functions through the release of neurotransmitters. The present study was designed to determine, for the first time, the changes in pituitary adenylate cyclase-activating polypeptide (PACAP), substance P (SP) and galanin (GAL) expression in porcine jejunum after long-term treatment with aspirin, indomethacin and naproxen. The study was performed on 16 immature pigs. The animals were randomly divided into four experimental groups: control, aspirin, indomethacin and naproxen. Control animals were given empty gelatin capsules, while animals in the test groups received selected NSAIDs for 28 days. Next, animals from each group were euthanized. Frozen sections were prepared from collected jejunum and subjected to double immunofluorescence staining. NSAIDs supplementation caused a significant increase in the population of PACAP-, SP- and GAL-containing enteric neurons in the porcine jejunum. Our results suggest the participation of the selected neurotransmitters in regulatory processes of the gastrointestinal function and may indicate the direct toxic effect of NSAIDs on the ENS neurons.
format	article
author	Marta Brzozowska Barbara Jana Jarosław Całka
author_facet	Marta Brzozowska Barbara Jana Jarosław Całka
author_sort	Marta Brzozowska
title	Effect of NSAIDs Supplementation on the PACAP-, SP- and GAL-Immunoreactive Neurons in the Porcine Jejunum
title_short	Effect of NSAIDs Supplementation on the PACAP-, SP- and GAL-Immunoreactive Neurons in the Porcine Jejunum
title_full	Effect of NSAIDs Supplementation on the PACAP-, SP- and GAL-Immunoreactive Neurons in the Porcine Jejunum
title_fullStr	Effect of NSAIDs Supplementation on the PACAP-, SP- and GAL-Immunoreactive Neurons in the Porcine Jejunum
title_full_unstemmed	Effect of NSAIDs Supplementation on the PACAP-, SP- and GAL-Immunoreactive Neurons in the Porcine Jejunum
title_sort	effect of nsaids supplementation on the pacap-, sp- and gal-immunoreactive neurons in the porcine jejunum
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/051ace0b69314c86bccf896030f3df6a
work_keys_str_mv	AT martabrzozowska effectofnsaidssupplementationonthepacapspandgalimmunoreactiveneuronsintheporcinejejunum AT barbarajana effectofnsaidssupplementationonthepacapspandgalimmunoreactiveneuronsintheporcinejejunum AT jarosławcałka effectofnsaidssupplementationonthepacapspandgalimmunoreactiveneuronsintheporcinejejunum
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Effect of NSAIDs Supplementation on the PACAP-, SP- and GAL-Immunoreactive Neurons in the Porcine Jejunum

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