Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.

Recent studies identified a 5´ to 3´ exoribonuclease termed Sso-RNase J in the crenarchaeon Sulfolobus solfataricus (Sso), which has been reclassified to the aCPSF2 (archaeal cleavage and polyadenylation specificity factor 2) group of β-CASP proteins. In this study, the Sso-aCPSF2 orthologue of Sulf...

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Autores principales:	Birgit Märtens, Fabian Amman, Salim Manoharadas, Lukas Zeichen, Alvaro Orell, Sonja-Verena Albers, Ivo Hofacker, Udo Bläsi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0521362450494f52906c6a85e96e0aa7
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spelling	oai:doaj.org-article:0521362450494f52906c6a85e96e0aa72021-11-18T08:52:13ZAlterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.1932-620310.1371/journal.pone.0076569https://doaj.org/article/0521362450494f52906c6a85e96e0aa72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24116119/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recent studies identified a 5´ to 3´ exoribonuclease termed Sso-RNase J in the crenarchaeon Sulfolobus solfataricus (Sso), which has been reclassified to the aCPSF2 (archaeal cleavage and polyadenylation specificity factor 2) group of β-CASP proteins. In this study, the Sso-aCPSF2 orthologue of Sulfolobus acidocaldarius (Saci-aCPSF2) was functionally characterized. Like Sso-aCPSF2, Saci-aCPSF2 degrades RNA with 5´ to 3´ directionality in vitro. To address the biological significance of Saci-aCPSF2, a deletion mutant was constructed, and the influence of Saci-aCPSF2 on the transcriptome profile was assessed employing high throughput RNA sequencing. This analysis revealed 560 genes with differential transcript abundance, suggesting a considerable role of this enzyme in RNA metabolism. In addition, bioinformatic analyses revealed several transcripts that are preferentially degraded at the 5´ end. This was exemplarily verified for two transcripts by Northern-blot analyses, showing for the first time that aCPSF2 proteins play a role in 5' to 3' directional mRNA decay in the crenarchaeal clade of Archaea.Birgit MärtensFabian AmmanSalim ManoharadasLukas ZeichenAlvaro OrellSonja-Verena AlbersIvo HofackerUdo BläsiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e76569 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Birgit Märtens Fabian Amman Salim Manoharadas Lukas Zeichen Alvaro Orell Sonja-Verena Albers Ivo Hofacker Udo Bläsi Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.
description	Recent studies identified a 5´ to 3´ exoribonuclease termed Sso-RNase J in the crenarchaeon Sulfolobus solfataricus (Sso), which has been reclassified to the aCPSF2 (archaeal cleavage and polyadenylation specificity factor 2) group of β-CASP proteins. In this study, the Sso-aCPSF2 orthologue of Sulfolobus acidocaldarius (Saci-aCPSF2) was functionally characterized. Like Sso-aCPSF2, Saci-aCPSF2 degrades RNA with 5´ to 3´ directionality in vitro. To address the biological significance of Saci-aCPSF2, a deletion mutant was constructed, and the influence of Saci-aCPSF2 on the transcriptome profile was assessed employing high throughput RNA sequencing. This analysis revealed 560 genes with differential transcript abundance, suggesting a considerable role of this enzyme in RNA metabolism. In addition, bioinformatic analyses revealed several transcripts that are preferentially degraded at the 5´ end. This was exemplarily verified for two transcripts by Northern-blot analyses, showing for the first time that aCPSF2 proteins play a role in 5' to 3' directional mRNA decay in the crenarchaeal clade of Archaea.
format	article
author	Birgit Märtens Fabian Amman Salim Manoharadas Lukas Zeichen Alvaro Orell Sonja-Verena Albers Ivo Hofacker Udo Bläsi
author_facet	Birgit Märtens Fabian Amman Salim Manoharadas Lukas Zeichen Alvaro Orell Sonja-Verena Albers Ivo Hofacker Udo Bläsi
author_sort	Birgit Märtens
title	Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.
title_short	Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.
title_full	Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.
title_fullStr	Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.
title_full_unstemmed	Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.
title_sort	alterations of the transcriptome of sulfolobus acidocaldarius by exoribonuclease acpsf2.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0521362450494f52906c6a85e96e0aa7
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Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.

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