Nucleic acid binding and other biomedical properties of artificial oligolysines

Giovanni N Roviello,1 Caterina Vicidomini,1 Vincenzo Costanzo,1 Valentina Roviello2 1CNR Istituto di Biostrutture e Bioimmagini, Via Mezzocannone site and Headquarters, 2Centro Regionale di Competenza (CRdC) Tecnologie, Via Nuova Agnano, Napoli, Italy Abstract: In the present study, we report the in...

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Autores principales:	Roviello GN, Vicidomini C, Costanzo V, Roviello V
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	nucleic acid binding polyinosinic acid double-stranded nucleic acids oligolysine circular dichroism Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/052bdc7c2d7e426f8cd229891315c9bf
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spelling	oai:doaj.org-article:052bdc7c2d7e426f8cd229891315c9bf2021-12-02T07:15:14ZNucleic acid binding and other biomedical properties of artificial oligolysines1178-2013https://doaj.org/article/052bdc7c2d7e426f8cd229891315c9bf2016-11-01T00:00:00Zhttps://www.dovepress.com/nucleic-acid-binding-and-other-biomedical-properties-of-artificial-oli-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Giovanni N Roviello,1 Caterina Vicidomini,1 Vincenzo Costanzo,1 Valentina Roviello2 1CNR Istituto di Biostrutture e Bioimmagini, Via Mezzocannone site and Headquarters, 2Centro Regionale di Competenza (CRdC) Tecnologie, Via Nuova Agnano, Napoli, Italy Abstract: In the present study, we report the interaction of an artificial oligolysine (referred to as AOL) realized in our laboratory with targets of biomedical importance. These included polyinosinic acid (poly rI) and its complex with polycytidylic acid (poly I:C), RNAs with well-known interferon-inducing ability, and double-stranded (ds) DNA. The ability of the peptide to bind both single-stranded poly rI and ds poly I:C RNAs emerged from our circular dichroism (CD) and ultraviolet (UV) studies. In addition, we found that AOL forms complexes with dsDNA, as shown by spectroscopic binding assays and UV thermal denaturation experiments. These findings are encouraging for the possible use of AOL in biomedicine for nucleic acid targeting and oligonucleotide condensation, with the latter being a key step preceding their clinical application. Moreover, we tested the ability of AOL to bind to proteins, using serum albumin as a model protein. We demonstrated the oligolysine–protein binding by CD experiments which suggested that AOL, positively charged under physiological conditions, binds to the protein regions rich in anionic residues. Finally, the morphology characterization of the solid oligolysine, performed by scanning electron microscopy, showed different crystal forms including cubic-shaped crystals confirming the high purity of AOL. Keywords: nucleic acid binding, polyinosinic acid, double-stranded nucleic acids, oligolysine, circular dichroismRoviello GNVicidomini CCostanzo VRoviello VDove Medical Pressarticlenucleic acid bindingpolyinosinic aciddouble-stranded nucleic acidsoligolysinecircular dichroismMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5897-5904 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	nucleic acid binding polyinosinic acid double-stranded nucleic acids oligolysine circular dichroism Medicine (General) R5-920
spellingShingle	nucleic acid binding polyinosinic acid double-stranded nucleic acids oligolysine circular dichroism Medicine (General) R5-920 Roviello GN Vicidomini C Costanzo V Roviello V Nucleic acid binding and other biomedical properties of artificial oligolysines
description	Giovanni N Roviello,1 Caterina Vicidomini,1 Vincenzo Costanzo,1 Valentina Roviello2 1CNR Istituto di Biostrutture e Bioimmagini, Via Mezzocannone site and Headquarters, 2Centro Regionale di Competenza (CRdC) Tecnologie, Via Nuova Agnano, Napoli, Italy Abstract: In the present study, we report the interaction of an artificial oligolysine (referred to as AOL) realized in our laboratory with targets of biomedical importance. These included polyinosinic acid (poly rI) and its complex with polycytidylic acid (poly I:C), RNAs with well-known interferon-inducing ability, and double-stranded (ds) DNA. The ability of the peptide to bind both single-stranded poly rI and ds poly I:C RNAs emerged from our circular dichroism (CD) and ultraviolet (UV) studies. In addition, we found that AOL forms complexes with dsDNA, as shown by spectroscopic binding assays and UV thermal denaturation experiments. These findings are encouraging for the possible use of AOL in biomedicine for nucleic acid targeting and oligonucleotide condensation, with the latter being a key step preceding their clinical application. Moreover, we tested the ability of AOL to bind to proteins, using serum albumin as a model protein. We demonstrated the oligolysine–protein binding by CD experiments which suggested that AOL, positively charged under physiological conditions, binds to the protein regions rich in anionic residues. Finally, the morphology characterization of the solid oligolysine, performed by scanning electron microscopy, showed different crystal forms including cubic-shaped crystals confirming the high purity of AOL. Keywords: nucleic acid binding, polyinosinic acid, double-stranded nucleic acids, oligolysine, circular dichroism
format	article
author	Roviello GN Vicidomini C Costanzo V Roviello V
author_facet	Roviello GN Vicidomini C Costanzo V Roviello V
author_sort	Roviello GN
title	Nucleic acid binding and other biomedical properties of artificial oligolysines
title_short	Nucleic acid binding and other biomedical properties of artificial oligolysines
title_full	Nucleic acid binding and other biomedical properties of artificial oligolysines
title_fullStr	Nucleic acid binding and other biomedical properties of artificial oligolysines
title_full_unstemmed	Nucleic acid binding and other biomedical properties of artificial oligolysines
title_sort	nucleic acid binding and other biomedical properties of artificial oligolysines
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/052bdc7c2d7e426f8cd229891315c9bf
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Nucleic acid binding and other biomedical properties of artificial oligolysines

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