Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments

Xiaoquan Ding, Siheng Wang, Wenhe Jin, Xingwang Liu, Jun Chen, Shiyi ChenCenter of Sports Medicine, Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai 200040, People’s Republic of ChinaPurpose: This study was designed to evaluate the in vitro and in vivo biocompati...

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Autores principales:	Ding X, Wang S, Jin W, Liu X, Chen J, Chen S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Simvastatin chitosan hydroxyapatite osteointegration polyethylene terephthalate Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0530bc83f0a548138b0a0cc9d9e2d5bc
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spelling	oai:doaj.org-article:0530bc83f0a548138b0a0cc9d9e2d5bc2021-12-02T01:05:05ZEncapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments1178-2013https://doaj.org/article/0530bc83f0a548138b0a0cc9d9e2d5bc2019-07-01T00:00:00Zhttps://www.dovepress.com/encapsulation-of-a-nanoporous-simvastatin-chitosan-composite-to-enhanc-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xiaoquan Ding, Siheng Wang, Wenhe Jin, Xingwang Liu, Jun Chen, Shiyi ChenCenter of Sports Medicine, Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai 200040, People’s Republic of ChinaPurpose: This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.Methods: This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA. A field emission scanning electron microscope was used to characterize the morphology, and the in vitro SV release profile was analyzed. MC3T3 cells were cocultured with SV/CS/PET-HA, CS/PET-HA and PET-HA to test their biocompatibility using CCK-8 tests. Osteogenic differentiation was investigated by the expression of marker genes using qPCR. Osteointegration was performed by implanting the PET ligaments into the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks. The bone-implant interface was evaluated by micro-computed tomography (micro-CT) and histological analysis.Results: The characteristic nanoporous structures mainly formed on the surface of the plasma-sprayed HA particles in the SV/CS/PET-HA and CS/PET-HA groups. The SV release test showed that the sustained release of simvastatin lasted for 25 days in the SV/CS/PET-HA group. The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups. The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.Conclusion: The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.Keywords: simvastatin, chitosan, hydroxyapatite, osteointegration, polyethylene terephthalate  Ding XWang SJin WLiu XChen JChen SDove Medical PressarticleSimvastatinchitosanhydroxyapatiteosteointegrationpolyethylene terephthalateMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4881-4893 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Simvastatin chitosan hydroxyapatite osteointegration polyethylene terephthalate Medicine (General) R5-920
spellingShingle	Simvastatin chitosan hydroxyapatite osteointegration polyethylene terephthalate Medicine (General) R5-920 Ding X Wang S Jin W Liu X Chen J Chen S Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments
description	Xiaoquan Ding, Siheng Wang, Wenhe Jin, Xingwang Liu, Jun Chen, Shiyi ChenCenter of Sports Medicine, Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai 200040, People’s Republic of ChinaPurpose: This study was designed to evaluate the in vitro and in vivo biocompatibility and osteointegration of plasma-sprayed hydroxyapatite (HA)-coated polyethylene terephthalate (PET) ligaments encapsulated with a simvastatin (SV)-chitosan (CS) composite.Methods: This study compared the in vitro and in vivo bone responses to three different PET ligaments: SV/CS/PET-HA, CS/PET-HA and PET-HA. A field emission scanning electron microscope was used to characterize the morphology, and the in vitro SV release profile was analyzed. MC3T3 cells were cocultured with SV/CS/PET-HA, CS/PET-HA and PET-HA to test their biocompatibility using CCK-8 tests. Osteogenic differentiation was investigated by the expression of marker genes using qPCR. Osteointegration was performed by implanting the PET ligaments into the proximal tibia bone tunnels of male Sprague-Dawley rats for 3 weeks and 6 weeks. The bone-implant interface was evaluated by micro-computed tomography (micro-CT) and histological analysis.Results: The characteristic nanoporous structures mainly formed on the surface of the plasma-sprayed HA particles in the SV/CS/PET-HA and CS/PET-HA groups. The SV release test showed that the sustained release of simvastatin lasted for 25 days in the SV/CS/PET-HA group. The in vitro studies demonstrated that the SV/CS/PET-HA ligaments induced osteogenic differentiation in the MC3T3 cells, with higher mRNA expression levels of collagen-1, bone morphogenetic protein-2, osteocalcin and alkaline phosphatase than those in the CS/PET-HA and PET-HA ligament groups. The in vivo tests showed that both micro-CT analysis (bone mineral density and bone volume per total volume) and histological analysis (bone implant contact and interface area) revealed significantly higher peri-implant bone formation and less interface area in the SV/CS/PET-HA group than in the other groups.Conclusion: The SV-CS composite nanoporous structure was associated with the improved biocompatibility and osteogenic differentiation in vitro and enhanced osteointegration process in vivo of plasma-sprayed HA-coated PET ligaments.Keywords: simvastatin, chitosan, hydroxyapatite, osteointegration, polyethylene terephthalate
format	article
author	Ding X Wang S Jin W Liu X Chen J Chen S
author_facet	Ding X Wang S Jin W Liu X Chen J Chen S
author_sort	Ding X
title	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments
title_short	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments
title_full	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments
title_fullStr	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments
title_full_unstemmed	Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments
title_sort	encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/0530bc83f0a548138b0a0cc9d9e2d5bc
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Encapsulation of a nanoporous simvastatin-chitosan composite to enhance osteointegration of hydroxyapatite-coated polyethylene terephthalate ligaments

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