Expression of genes encoding multi-transmembrane proteins in specific primate taste cell populations.

<h4>Background</h4>Using fungiform (FG) and circumvallate (CV) taste buds isolated by laser capture microdissection and analyzed using gene arrays, we previously constructed a comprehensive database of gene expression in primates, which revealed over 2,300 taste bud-associated genes. Bio...

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Autores principales:	Bryan D Moyer, Peter Hevezi, Na Gao, Min Lu, Dalia Kalabat, Hortensia Soto, Fernando Echeverri, Bianca Laita, Shaoyang Anthony Yeh, Mark Zoller, Albert Zlotnik
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05348aff99054d6581aa73ab64d722fa
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spelling	oai:doaj.org-article:05348aff99054d6581aa73ab64d722fa2021-11-25T06:27:35ZExpression of genes encoding multi-transmembrane proteins in specific primate taste cell populations.1932-620310.1371/journal.pone.0007682https://doaj.org/article/05348aff99054d6581aa73ab64d722fa2009-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19997627/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Using fungiform (FG) and circumvallate (CV) taste buds isolated by laser capture microdissection and analyzed using gene arrays, we previously constructed a comprehensive database of gene expression in primates, which revealed over 2,300 taste bud-associated genes. Bioinformatics analyses identified hundreds of genes predicted to encode multi-transmembrane domain proteins with no previous association with taste function. A first step in elucidating the roles these gene products play in gustation is to identify the specific taste cell types in which they are expressed.<h4>Methodology/principal findings</h4>Using double label in situ hybridization analyses, we identified seven new genes expressed in specific taste cell types, including sweet, bitter, and umami cells (TRPM5-positive), sour cells (PKD2L1-positive), as well as other taste cell populations. Transmembrane protein 44 (TMEM44), a protein with seven predicted transmembrane domains with no homology to GPCRs, is expressed in a TRPM5-negative and PKD2L1-negative population that is enriched in the bottom portion of taste buds and may represent developmentally immature taste cells. Calcium homeostasis modulator 1 (CALHM1), a component of a novel calcium channel, along with family members CALHM2 and CALHM3; multiple C2 domains; transmembrane 1 (MCTP1), a calcium-binding transmembrane protein; and anoctamin 7 (ANO7), a member of the recently identified calcium-gated chloride channel family, are all expressed in TRPM5 cells. These proteins may modulate and effect calcium signalling stemming from sweet, bitter, and umami receptor activation. Synaptic vesicle glycoprotein 2B (SV2B), a regulator of synaptic vesicle exocytosis, is expressed in PKD2L1 cells, suggesting that this taste cell population transmits tastant information to gustatory afferent nerve fibers via exocytic neurotransmitter release.<h4>Conclusions/significance</h4>Identification of genes encoding multi-transmembrane domain proteins expressed in primate taste buds provides new insights into the processes of taste cell development, signal transduction, and information coding. Discrete taste cell populations exhibit highly specific gene expression patterns, supporting a model whereby each mature taste receptor cell is responsible for sensing, transmitting, and coding a specific taste quality.Bryan D MoyerPeter HeveziNa GaoMin LuDalia KalabatHortensia SotoFernando EcheverriBianca LaitaShaoyang Anthony YehMark ZollerAlbert ZlotnikPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 12, p e7682 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bryan D Moyer Peter Hevezi Na Gao Min Lu Dalia Kalabat Hortensia Soto Fernando Echeverri Bianca Laita Shaoyang Anthony Yeh Mark Zoller Albert Zlotnik Expression of genes encoding multi-transmembrane proteins in specific primate taste cell populations.
description	<h4>Background</h4>Using fungiform (FG) and circumvallate (CV) taste buds isolated by laser capture microdissection and analyzed using gene arrays, we previously constructed a comprehensive database of gene expression in primates, which revealed over 2,300 taste bud-associated genes. Bioinformatics analyses identified hundreds of genes predicted to encode multi-transmembrane domain proteins with no previous association with taste function. A first step in elucidating the roles these gene products play in gustation is to identify the specific taste cell types in which they are expressed.<h4>Methodology/principal findings</h4>Using double label in situ hybridization analyses, we identified seven new genes expressed in specific taste cell types, including sweet, bitter, and umami cells (TRPM5-positive), sour cells (PKD2L1-positive), as well as other taste cell populations. Transmembrane protein 44 (TMEM44), a protein with seven predicted transmembrane domains with no homology to GPCRs, is expressed in a TRPM5-negative and PKD2L1-negative population that is enriched in the bottom portion of taste buds and may represent developmentally immature taste cells. Calcium homeostasis modulator 1 (CALHM1), a component of a novel calcium channel, along with family members CALHM2 and CALHM3; multiple C2 domains; transmembrane 1 (MCTP1), a calcium-binding transmembrane protein; and anoctamin 7 (ANO7), a member of the recently identified calcium-gated chloride channel family, are all expressed in TRPM5 cells. These proteins may modulate and effect calcium signalling stemming from sweet, bitter, and umami receptor activation. Synaptic vesicle glycoprotein 2B (SV2B), a regulator of synaptic vesicle exocytosis, is expressed in PKD2L1 cells, suggesting that this taste cell population transmits tastant information to gustatory afferent nerve fibers via exocytic neurotransmitter release.<h4>Conclusions/significance</h4>Identification of genes encoding multi-transmembrane domain proteins expressed in primate taste buds provides new insights into the processes of taste cell development, signal transduction, and information coding. Discrete taste cell populations exhibit highly specific gene expression patterns, supporting a model whereby each mature taste receptor cell is responsible for sensing, transmitting, and coding a specific taste quality.
format	article
author	Bryan D Moyer Peter Hevezi Na Gao Min Lu Dalia Kalabat Hortensia Soto Fernando Echeverri Bianca Laita Shaoyang Anthony Yeh Mark Zoller Albert Zlotnik
author_facet	Bryan D Moyer Peter Hevezi Na Gao Min Lu Dalia Kalabat Hortensia Soto Fernando Echeverri Bianca Laita Shaoyang Anthony Yeh Mark Zoller Albert Zlotnik
author_sort	Bryan D Moyer
title	Expression of genes encoding multi-transmembrane proteins in specific primate taste cell populations.
title_short	Expression of genes encoding multi-transmembrane proteins in specific primate taste cell populations.
title_full	Expression of genes encoding multi-transmembrane proteins in specific primate taste cell populations.
title_fullStr	Expression of genes encoding multi-transmembrane proteins in specific primate taste cell populations.
title_full_unstemmed	Expression of genes encoding multi-transmembrane proteins in specific primate taste cell populations.
title_sort	expression of genes encoding multi-transmembrane proteins in specific primate taste cell populations.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/05348aff99054d6581aa73ab64d722fa
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Expression of genes encoding multi-transmembrane proteins in specific primate taste cell populations.

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