In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media

Abstract. Isnaini Y, Praptosuwiryo TNg. 2020. In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media. Biodiversitas 21: 5373-5381. Cibotium barometz (L.) J. Sm. is known as the golden chicken fern and included in Appendix II of CITES. It is a...

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Autores principales:	Yupi ISNAINI, Titien Ngatinem Praptosuwiryo
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	ex-situ conservation, medicinal fern, propagation, spore culture Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/053903dc9cca4b269d49d10f77168b82
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spelling	oai:doaj.org-article:053903dc9cca4b269d49d10f77168b822021-11-22T00:44:11ZIn vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media1412-033X2085-472210.13057/biodiv/d211143https://doaj.org/article/053903dc9cca4b269d49d10f77168b822020-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6877https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Isnaini Y, Praptosuwiryo TNg. 2020. In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media. Biodiversitas 21: 5373-5381. Cibotium barometz (L.) J. Sm. is known as the golden chicken fern and included in Appendix II of CITES. It is an important export commodity for traditional and modern medicine. Globally, populations of this species are under significant pressure due to overexploitation in the wild. In vitro culture is one of the technologies used for ex-situ propagation and conservation of rare and endangered ferns and lycophytes. This study’s objectives were: (i) to observe in vitro spore germination and early gametophyte development of C. barometz, and (ii) to determine the best culture medium for rapid spore germination and early development of the gametophytes. The sterilized spores were sown in half-strength Murashige & Skoog (½MS) basal medium supplemented with combinations of 6-Benzylaminopurine (BAP) and ?-Naphthalene acetic acid (NAA). A factorial combination of four BAP concentrations (0, 2, 4, and 6 mg L-1) with four concentrations of NAA (0; 0.01; 0.03 and 0.05 mg L-1) created 16 treatments replicated in a Completely Randomized Design. Spore germination of C. barometz was observed to be Vittaria-type, and its prothallial development was Drynaria-type. Spore germination started 7-14 days after sowing. Young heart-shape gametophytes consisting of 110-240 cells were formed in 45-61 days after sowing. The two best spore culture media for rapid spore germination and development of C. barometz gametophytes were ½ MS with or without 2 mg L-1 BAP.Yupi ISNAINITitien Ngatinem PraptosuwiryoMBI & UNS Soloarticleex-situ conservation, medicinal fern, propagation, spore cultureBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	ex-situ conservation, medicinal fern, propagation, spore culture Biology (General) QH301-705.5
spellingShingle	ex-situ conservation, medicinal fern, propagation, spore culture Biology (General) QH301-705.5 Yupi ISNAINI Titien Ngatinem Praptosuwiryo In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media
description	Abstract. Isnaini Y, Praptosuwiryo TNg. 2020. In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media. Biodiversitas 21: 5373-5381. Cibotium barometz (L.) J. Sm. is known as the golden chicken fern and included in Appendix II of CITES. It is an important export commodity for traditional and modern medicine. Globally, populations of this species are under significant pressure due to overexploitation in the wild. In vitro culture is one of the technologies used for ex-situ propagation and conservation of rare and endangered ferns and lycophytes. This study’s objectives were: (i) to observe in vitro spore germination and early gametophyte development of C. barometz, and (ii) to determine the best culture medium for rapid spore germination and early development of the gametophytes. The sterilized spores were sown in half-strength Murashige & Skoog (½MS) basal medium supplemented with combinations of 6-Benzylaminopurine (BAP) and ?-Naphthalene acetic acid (NAA). A factorial combination of four BAP concentrations (0, 2, 4, and 6 mg L-1) with four concentrations of NAA (0; 0.01; 0.03 and 0.05 mg L-1) created 16 treatments replicated in a Completely Randomized Design. Spore germination of C. barometz was observed to be Vittaria-type, and its prothallial development was Drynaria-type. Spore germination started 7-14 days after sowing. Young heart-shape gametophytes consisting of 110-240 cells were formed in 45-61 days after sowing. The two best spore culture media for rapid spore germination and development of C. barometz gametophytes were ½ MS with or without 2 mg L-1 BAP.
format	article
author	Yupi ISNAINI Titien Ngatinem Praptosuwiryo
author_facet	Yupi ISNAINI Titien Ngatinem Praptosuwiryo
author_sort	Yupi ISNAINI
title	In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media
title_short	In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media
title_full	In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media
title_fullStr	In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media
title_full_unstemmed	In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media
title_sort	in vitro spore germination and early gametophyte development of cibotium barometz (l.) j. sm. in different media
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/053903dc9cca4b269d49d10f77168b82
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In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media

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