Mangiferin Inhibits Apoptosis and Autophagy Induced by Staphylococcus aureus in RAW264.7 Cells

Jun Xu,* Hua Yao,* Shichen Wang,* Huanrong Li, Xiaolin Hou Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, National Demonstration Center for Experimental Animal Education, Department of Veterinary Medicine, Beijing University of Agriculture, Beijing, People’s Republic of...

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Autores principales:	Xu J, Yao H, Wang S, Li H, Hou X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	mangiferin staphylococcus aureus apoptosis autophagy Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/053f9b24f6a840918f7e81293ab82bd2
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spelling	oai:doaj.org-article:053f9b24f6a840918f7e81293ab82bd22021-12-02T11:22:39ZMangiferin Inhibits Apoptosis and Autophagy Induced by Staphylococcus aureus in RAW264.7 Cells1178-7031https://doaj.org/article/053f9b24f6a840918f7e81293ab82bd22020-11-01T00:00:00Zhttps://www.dovepress.com/mangiferin-inhibits-apoptosis-and-autophagy-induced-by-staphylococcus--peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Jun Xu,* Hua Yao,* Shichen Wang,* Huanrong Li, Xiaolin Hou Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, National Demonstration Center for Experimental Animal Education, Department of Veterinary Medicine, Beijing University of Agriculture, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaolin Hou Tel +86 13521095760Email hxlsx@163.comPurpose: Staphylococcus aureus (S. aureus) is an important bacterial pathogen, which creates infective inflammation to human being and animals. Mangiferin (MG) is one of the natural flavonoids with anti-inflammatory, anti-bacterial, and anti-oxidative properties. However, the anti-apoptosis and anti-autophagy of MG are unknown. Hence, this study was aimed to research the inhibition of MG on S. aureus-induced apoptosis and autophagy in RAW264.7 cells.Methods: The RAW264.7 cells were pretreated with MG, or pretreated with SP600125 or anisomycin synchronously, and then infected with S. aureus (MOI=100:1). The viability and proliferation status of RAW264.7 cells were detected by MTT and EdU assay. The relative expression of TNF-α, IL-6 and IL-10 protein was tested with ELISA. The levels of Bax, Bcl-2, caspase-3, c-Jun N-terminal kinase (JNK), extracellular-regulated protein kinase (ERK), p38, LC3, Beclin-1, p62, phosphorylated JNK, phosphorylated p38 and phosphorylated ERK in cells were detected by Western blotting. The apoptosis rate of RAW264.7 cells was analyzed by flow cytometric assay.Results: The study showed that MG significantly attenuated RAW264.7 cells apoptosis and autophagy caused by S. aureus. MG alleviated S. aureus-induced apoptosis by down-regulating the protein level of active caspase-3 and Bax and up-regulating the level of Bcl-2. MG also inhibited S. aureus-induced autophagy via decreasing the protein level of LC3-II/LC3-I and Beclin-1 or increasing the protein expression of p62. This protective role was dependent on the up-regulation of JNK signal pathway, which was confirmed by using JNK agonist and inhibitor.Conclusion: Our results demonstrated that MG might protect RAW264.7 cells from S. aureus-induced apoptosis and autophagy via inhibiting JNK/Bax-dependent signal pathway. Therefore, MG may be a potential agent against pathological cell damage induced by S. aureus infection.Keywords: mangiferin, Staphylococcus aureus, apoptosis, autophagyXu JYao HWang SLi HHou XDove Medical Pressarticlemangiferinstaphylococcus aureusapoptosisautophagyPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 13, Pp 847-857 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	mangiferin staphylococcus aureus apoptosis autophagy Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	mangiferin staphylococcus aureus apoptosis autophagy Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Xu J Yao H Wang S Li H Hou X Mangiferin Inhibits Apoptosis and Autophagy Induced by Staphylococcus aureus in RAW264.7 Cells
description	Jun Xu,* Hua Yao,* Shichen Wang,* Huanrong Li, Xiaolin Hou Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, National Demonstration Center for Experimental Animal Education, Department of Veterinary Medicine, Beijing University of Agriculture, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaolin Hou Tel +86 13521095760Email hxlsx@163.comPurpose: Staphylococcus aureus (S. aureus) is an important bacterial pathogen, which creates infective inflammation to human being and animals. Mangiferin (MG) is one of the natural flavonoids with anti-inflammatory, anti-bacterial, and anti-oxidative properties. However, the anti-apoptosis and anti-autophagy of MG are unknown. Hence, this study was aimed to research the inhibition of MG on S. aureus-induced apoptosis and autophagy in RAW264.7 cells.Methods: The RAW264.7 cells were pretreated with MG, or pretreated with SP600125 or anisomycin synchronously, and then infected with S. aureus (MOI=100:1). The viability and proliferation status of RAW264.7 cells were detected by MTT and EdU assay. The relative expression of TNF-α, IL-6 and IL-10 protein was tested with ELISA. The levels of Bax, Bcl-2, caspase-3, c-Jun N-terminal kinase (JNK), extracellular-regulated protein kinase (ERK), p38, LC3, Beclin-1, p62, phosphorylated JNK, phosphorylated p38 and phosphorylated ERK in cells were detected by Western blotting. The apoptosis rate of RAW264.7 cells was analyzed by flow cytometric assay.Results: The study showed that MG significantly attenuated RAW264.7 cells apoptosis and autophagy caused by S. aureus. MG alleviated S. aureus-induced apoptosis by down-regulating the protein level of active caspase-3 and Bax and up-regulating the level of Bcl-2. MG also inhibited S. aureus-induced autophagy via decreasing the protein level of LC3-II/LC3-I and Beclin-1 or increasing the protein expression of p62. This protective role was dependent on the up-regulation of JNK signal pathway, which was confirmed by using JNK agonist and inhibitor.Conclusion: Our results demonstrated that MG might protect RAW264.7 cells from S. aureus-induced apoptosis and autophagy via inhibiting JNK/Bax-dependent signal pathway. Therefore, MG may be a potential agent against pathological cell damage induced by S. aureus infection.Keywords: mangiferin, Staphylococcus aureus, apoptosis, autophagy
format	article
author	Xu J Yao H Wang S Li H Hou X
author_facet	Xu J Yao H Wang S Li H Hou X
author_sort	Xu J
title	Mangiferin Inhibits Apoptosis and Autophagy Induced by Staphylococcus aureus in RAW264.7 Cells
title_short	Mangiferin Inhibits Apoptosis and Autophagy Induced by Staphylococcus aureus in RAW264.7 Cells
title_full	Mangiferin Inhibits Apoptosis and Autophagy Induced by Staphylococcus aureus in RAW264.7 Cells
title_fullStr	Mangiferin Inhibits Apoptosis and Autophagy Induced by Staphylococcus aureus in RAW264.7 Cells
title_full_unstemmed	Mangiferin Inhibits Apoptosis and Autophagy Induced by Staphylococcus aureus in RAW264.7 Cells
title_sort	mangiferin inhibits apoptosis and autophagy induced by staphylococcus aureus in raw264.7 cells
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/053f9b24f6a840918f7e81293ab82bd2
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_version_	1718395919096872960

Mangiferin Inhibits Apoptosis and Autophagy Induced by Staphylococcus aureus in RAW264.7 Cells

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